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Uridinediphosphate-glucose: Isovitexin 7-O-glucosyltransferase from barley protoplasts: Subcellular localization

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Abstract

Protoplasts isolated from 6-d-old primary leaves of barley (Hordeum vulgare L.) contain an enzyme which transfers the glucosyl moiety of uridine-diphosphateglucose to isovitexin, resulting in the formation of saponarin, the major flavonoid of barley. Purified chloroplasts isolated from protoplasts contained less than 2% of the total glucosyltransferase activity. These chloroplasts were 97% intact, based on ribulose-bisphosphate-carboxylase activity. Similarly low levels of glucosyltransferase activity were found in mitochondria and microbody or microsomal preparations from protoplasts. The soluble fraction (cytosol) contained at least 93% of the isovitexin 7-O-glucosyltransferase activity.

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References

  • Alibert, G., Ranjeva, R., Boudet, A.M.: Organisation subcellulaire des voies de synthèse des composés phénoliques. Physiol. Vég. 15, 279–301 (1977)

    Google Scholar 

  • Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949)

    Google Scholar 

  • Brederode, J. van, Nigtevecht, G. van: Identification, properties and genetic control of UDP-glucose: isovitexin 7-O-glucosyl-transferase isolated from petals of Melandrium album. Mol. Gen. Genet. 122, 215–229 (1973)

    PubMed  Google Scholar 

  • Elias, B.A., Givan, C.V.: Density gradient and differential centrifugation methods for chloroplast purification and enzyme localization in leaf tissue. Planta 142, 31–320 (1978)

    Google Scholar 

  • Hahlbrock, K., Grisebach, H.: Biosynthesis of flavonoids. In: The Flavonoids, pp. 866–915, Harborne, J., Mabry, T., Mabry, H., eds. London: Chapman and Hall 1975

    Google Scholar 

  • Herdt, E., Wiermann, R.: Die subzelluläre Lokalisation von Enzymen des Phenylpropanoidstoffwechsels im Antherentapetum: Phenylanin-Ammonium-Lyase, Zimtsäure 4-Hydroxylase, SAM: Kaffeesäure 3-O-Methyltransferase. Protoplasma 96, 283–292 (1978)

    Google Scholar 

  • Hrazdina, G., Wagner, G.J., Siegelman, H.W.: Subcellular localization of enzymes of anthocyanin biosynthesis in protoplasts. Phytochemistry 17, 53–56 (1978)

    Article  Google Scholar 

  • Miflin, B.J., Beevers, H.: Isolation of intact plastids from a range of plant tissues. Plant Physiol. 53, 870–874 (1974)

    Google Scholar 

  • Monties, B.: Sur la présence de composés phénoliques dans les chloroplastes d'Angiospermes. Physiol. Vég. 15, 29–45 (1969)

    Google Scholar 

  • Ranjeva, R., Alibert, G., Boudet, A.M.: Metabolisme des composés phénoliques chez le Pétunia. VI. Intervention des chloroplastes dans la biosynthese de la naringénine et de l'acide chlorogénique. Plant Sci. Lett. 10, 235–242 (1977)

    Google Scholar 

  • Rathnam, C.K.M., Edwards, G.E.: Protoplasts as a tool for isolating functional chloroplasts from leaves. Plant Cell Physiol. 17, 177–186 (1976)

    Google Scholar 

  • Saunders, J.A., Conn, E.E.: Presence of the cyanogenic glucoside dhurrin in isolated vacuoles from Sorghum. Plant Physiol. 61, 154–157 (1978)

    Google Scholar 

  • Saunders, J.A., McClure, J.W.: Phytochrome controlled phenylalanine ammonia lyase in Hordeum vulgare plastids. Phytochemistry 14, 1285–1289 (1975)

    Article  Google Scholar 

  • Saunders, J.A., McClure, J.W.: The distribution of flavonoids in chloroplasts of twenty five species of vascular plants. Phytochemistry 15, 809–810 (1976)

    Article  Google Scholar 

  • Saunders, J.A., McClure, J.W., Wallace, J.W.: The subcellular localization of enzymes in the flavonoid biosynthetic pathway. (Abstr.) Am. J. Bot. 60, Suppl., 29 (1973)

    Google Scholar 

  • Seikel, M.K., Geissman, T.A.: The flavonoid constituents of barley (Hordeum vulgare). I. Saponarin. Arch. Biochem. Biophys. 71, 17–30 (1957)

    PubMed  Google Scholar 

  • Weissenböck, G., Plesser, A., Trinks, K.: Flavonoidgehalt und Enzymaktivitäten isolierten Haferchloroplasten (Avena sativa L.). Ber. Dtsch. Bot. Ges. 89, 457–472 (1976)

    Google Scholar 

  • Willmer, C.M., Pallas, J.E., Jr., Black, C.C., Jr.: Carbon dioxide metabolism in leaf epidermal tissue. Plant Physiol. 52, 448–452 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, Miami University, 45056, Oxford, OH, USA

    Denise E. Blume & Jerry W. McClure

  2. Department of Chemistry, Miami University, 45056, Oxford, OH, USA

    Jan G. Jaworski

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  1. Denise E. Blume
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  2. Jan G. Jaworski
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  3. Jerry W. McClure
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Blume, D.E., Jaworski, J.G. & McClure, J.W. Uridinediphosphate-glucose: Isovitexin 7-O-glucosyltransferase from barley protoplasts: Subcellular localization. Planta 146, 199–202 (1979). https://doi.org/10.1007/BF00388232

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  • DOI: https://doi.org/10.1007/BF00388232

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