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Transcript analysis of TR DNA in octopine-type crown gall tumors

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Summary

The sizes and map positions of five polyadenylated transcripts from the TR region of the octopine-type tobacco crown gall tumor E9 have been determined by Northern blot and S1 nuclease analyses. These RNAs range in size from 780 to 1,600 bases, and their relative abundancies vary according to the growth conditions of the tumor cultures. Different relative abundancies of these transcripts have also been detected in the tobacco tumor line 15955/1 and the sunflower tumor lines 15W, S4-2, and S4-4. The possible functions of several of these TR T-DNA genes are discussed.

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Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, 47907, West Lafayette, Indiana, USA

    Susan J. Karcher, Victor J. DiRita & Stanton B. Gelvin

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  1. Susan J. Karcher
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  2. Victor J. DiRita
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  3. Stanton B. Gelvin
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Communicated by R. Goldberg

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Karcher, S.J., DiRita, V.J. & Gelvin, S.B. Transcript analysis of TR DNA in octopine-type crown gall tumors. Molec. Gen. Genet. 194, 159–165 (1984). https://doi.org/10.1007/BF00383511

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  • DOI: https://doi.org/10.1007/BF00383511

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