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Identification of internal poly(A) tracts (IPATs) of variable lengths in a novel tobacco rattle virus RNA2 in potatoes

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Abstract

The nucleotide (nt) sequences of two closely related isolates (CeWF-2 and CeWGH-2) of a novel tobacco rattle virus (TRV) RNA2 were determined. The sequences of their RNA2-specific regions were almost identical and contained four open reading frames (ORFs) in an arrangement similar to that found in the previously described TRV TpO1 RNA2. Their predicted ORF 1 gene products shared 97 % amino acid sequence identity with the TpO1 coat protein, but the ORF 2 and ORF 3 gene products shared only 82 % sequence identity, and no appreciable sequence similarity was found between the CeWF-2/CeWGH-2 and TpO1 ORF 4 gene products. In the CeWGH-2 sequence, the RNA2-specific and RNA1-related regions were separated by seven adenine (A) residues. In CeWF-2, however, an internal poly(A) tract (IPAT) of variable size consisting of ca. 20 to 30 (A) residues was found. This is the first report of an IPAT occurring in a tobravirus RNA2.

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Acknowledgements

The financial support given by the Gisela und Hermann Stegemann-Stiftung is gratefully acknowledged.

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Authors and Affiliations

  1. c/o Julius Kühn Institute, Federal Research Center for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11, 38104, Braunschweig, Germany

    R. Koenig

  2. Julius Kühn Institute, Federal Research Center for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11, 38104, Braunschweig, Germany

    H. Ziebell

  3. Julius Kühn Institute, Federal Research Center for Cultivated Plants, Institute for Plant Protection in Field Crops and Grassland, Messeweg 11, 38104, Braunschweig, Germany

    I. Hilbrich & K. Lindner

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  1. R. Koenig
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  2. H. Ziebell
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  3. I. Hilbrich
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  4. K. Lindner
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Correspondence to R. Koenig.

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None of the authors received any kind of grant for these studies. This article does not contain any studies with human participants or animals performed by any of the authors.

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Koenig, R., Ziebell, H., Hilbrich, I. et al. Identification of internal poly(A) tracts (IPATs) of variable lengths in a novel tobacco rattle virus RNA2 in potatoes. Arch Virol 162, 295–297 (2017). https://doi.org/10.1007/s00705-016-3041-2

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  • DOI: https://doi.org/10.1007/s00705-016-3041-2

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