Summary
Several nopaline degrading strains and one octopine degrading strain are shown to loose oncogenicity as well as the ability to utilize these guanidine compounds when they are cured of their TI plasmid. To investigate whether the specific genes involved in the utilization of one or the other compound are located on the plasmid, plasmid-transfer experiments have been performed.
The plasmid from a nopaline degrading strain has been transferred to a naturally non oncogenic Agrobacterium namely A. radiobacter. Furthermore, the plasmid from an octopine degrading strain has been transferred to a plasmid-cured strain which originally had the capacity to utilize nopaline. Both kinds of experiments prove that the TI plasmid determines the strain specificity with regard to the utilization of either octopine or nopaline.
They also demonstrate that the synthesis of either octopine or nopaline in crown gall cells is also determined by genes located on the TI plasmid harboured by the transforming A. tumefaciens strains.
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Biemann, K., Lioret, C., Asselineau, K. Lederer, E., Polonski, T.: Sur la structure chimique de la lysopine, nouvel acide aminé isolé du tissue de Crown gall. Bull. Soc. Chim. biol. (Paris) 42, 979 (1960)
Bomhoff, G.H.: Studies on Crown gall—a plant tumor. Investigations on protein composition and on the use of guanidine compounds as a marker for transformed cells. Thesis, Leiden (1974)
Datta, N., Hedges, R.W.: Host ranges of R factors. J. gen. Microbiol. 70, 453 (1972)
Dons, J.J.M., Valentijn, M., Schilperoort, R.A., Duijn, P.van: Nuclear DNA content and phytohormone requirements of normal crown gall and habituated tissues of Nicotiana tabacum var. White Burley. Exp. Cell Res. 89, 283 (1974)
Engler, G., Hernalsteens, J.P., Holsters, M., Montagu, M.van, Schilperoort, R.A., Schell, J.: Agrocin 84 Sensitivity: A plasmid determined property in Agrobacterium tumefaciens. Molec. gen. Genet. 138, 345 (1975)
Goldmann, A., Thomas, D.W., Morel, G.: Sur la structure de la nopaline, metabolite anormale de certaines tumeurs de Crown gall. C.R. Acad. Sci. (Paris), sér. D 268, 852 (1969)
Goldmann-Ménagé, A.: Recherches sur le métabolisme azoté des tissue de Crown gall cutivés in vitro. Thèse, Paris. Ann. Sci. Nat. Bot. Paris, 12e sér. 11, 223 (1970)
Hamilton, R.H., Fall, M.Z.: The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia (Basel) 27, 229 (1971)
Johnson, R., Guderian, R.H., Eden, F., Chilton, M.D., Gordon, H.P., Nester, E.W.: Detection and quantitation of octopine in normal plant tissue and Crown gall tumor tissue. Proc. nat. Acad. Sci. (Wash.) 71, 536 (1974)
Kersters, K., Ley, J. de, Sneath, P.H.A., Sackin, M.: Numerical taxonomic analysis of Agrobacterium. J. gen. Microbiol. 78, 227 (1973)
Larebeke, N.van, Engler, G., Holsters, M., Elsacker, S.v.d., Zaenen, I., Schilperoort, R.A., Schell, J.: Large plasmid in Agrobacterium tumefaciens essential for Crown gall-inducing ability. Nature (Lond.) 252, 169 (1974)
Larebeke, N.van, Genetello, Ch., Schell, J., Schilperoort, R.A., Hermans, A.K., Hernalsteens, J.P., Montagu, M.van. Acquisition of tumor-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (Lond.) 255, 742 (1975)
Lioret, C.: Sur la mise en évidence d'un acide aminé non identifié particulier aux tissus de Crown gall. Bull. Soc. franç. Physiol. vég. 2, (1956)
Lippencott, J.A., Beiderbeck, R., Lippencott, B.B.: Utilization of octopine and nopaline by Agrobacterium. J. Bact. 116, 378 (1973)
Ménagé, A., Morel, G.: Sur la présence d'octopine dans les tissus de Crown gall. C.R. Acad. Sci. (Paris), sér. D 259, 4795 (1964)
Ménagé, A., Morel, G.: Sur la présence d'un acide aminé nouveau dans les tissus de Crown gall. C.R. Acad. Sci. (Paris), sér. D 261, 2001 (1965)
Petit, A., Delhaye, S., Tempe, J., Morel, G.: Recherches sur les guanidines des tissus de Crown gall. Mise en évidence d'une relation biochimique spécifique entre les souches d'Agrobacterium tumefaciens et les tumeurs qu'elles induisent. Physiol. Vég. 8, 205 (1970)
Petit, A., Tourneur, J.: Perte de virulence associée à la perte d'une activité enzymatique chez Agrobacterium tumefaciens. C.R. Acad. Sci. (Paris), sér. D 275, 137 (1972)
Rosenberg, H., Ennor, A.H., Morrison, J.F.: The estimation of arginine. Biochem. J. 63, 153 (1956)
Schell, J.: The role of plasmids in Crown gall formation by A. tumefaciens. Genetic Manipulations with plant material. Ed. L. Ledoux, p. 163. New York: Plenum Press 1975
Schilperoort, R.A.: Investigations on plant tumors—Crown gall—On the biochemistry of tumor induction by Agrobacterium tumefaciens. Thesis, Leiden (1969)
Schilperoort, R.A., Bomhoff, G.H.: Crown gall: A model for tumor research and Genetic engineering. Part. 1. General consideration about the Crown gall disease. Part. 2. The nature of the tumor-inducing-principle claborated by A. tumefaciens. Genetic manipulations with plant material ed. L. Ledoux, p. 143, New York: Plenum Press 1975
Watson, B., Currier, T.C., Gordon, M.P., Chilton, M.D., Nester, E.W.: Plasmid required for virulence of Agrobacterium tumefaciens. J. Bact. 123, 255 (1975)
Wendt-Gallitelli, H.F., Dobrigkeit, I.: Investigations implying the ivalidity of octopine as a marker for transformation by Agrobacterium tumefaciens. Z. Naturforsch. 28c, 768 (1973)
Zaenen, I., Larebeke, N.van, Teuchy, H., Schell, J.: Supercoiled circular DNA in Crown gall inducing Agrobacterium strains. J. molec. Biol. 86, 109 (1974)
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Bomhoff, G., Klapwijk, P.M., Kester, H.C.M. et al. Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens . Molec. Gen. Genet. 145, 177–181 (1976). https://doi.org/10.1007/BF00269591
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DOI: https://doi.org/10.1007/BF00269591