Abstract
Genomic DNA was isolated from 29 t strains and 4 congenic lines of mice, digested with restriction endonucleases, and hybridized with a probe representing the complement component 4 (C4) gene. All but one of the enzymes revealed restriction fragment length polymorphism in this sample of C4-related genes. Double digestion analysis suggested the presence of three C4 gene copies in some of the t chromosomes and two copies in others. The enzymes distinguished 16 different haplotypes among the 33 strains tested. Based on their restriction fragment length patterns, the t strains could be divided into four groups with strains in each group more closely related to each other with respect to their C4-region genes than strains belonging to different groups. At least three of these four groups represent different branches of the evolutionary tree constructed for the t chromosomes. The C4-related genes of the chromosomes are in strong linkage disequilibrium with the class II genes of the H-2 complex. Typing for the Ss and Slp allotypes of C4 has revealed the presence of the Ss1 phenotype in two t strains and of the Slpa phenotype in one strain.
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Alper, C. A.: Complement and the Mhe. In M. E. Dorf (ed.): The Role of the Major Histocompatibility Complex in Immunobiology, pp. 173–200, Garland, New York, 1981
Atkinson, J. P., Karp, D. R., Seeskin, E. P., Killion, C. C., Rosa, P. A., Newell, S. L., and Shreffler, D. C.: H2 S region determined polymorphic variants of the C4, Slp, C2, and B complement proteins. A compilation. Immunogenetics 16: 617–623, 1982
Carrol, M. C. and Capra, J. D.: Studies on murine Ss protein: Demonstration that S region encodes structural gene for fourth component of complement. Proc. Natl. Acad. Sci. U.S.A. 76: 4641–4645, 1979
Chaplin, D. D., Woods, D. E., Whitehead, A. S., Goldberger, G., Colten, H. R., and Seidman, J. G.: Molecular map of the murine S region. Proc. Natl. Acad. Sci. U.S.A. 80: 6947–6951, 1983
Dembié, Z., Singer, P., and Klein, J.: E°: A history of a mutation. EMBO J. 3: 1647–1654, 1984
Dembié, Z., Ayane, M., Klein, J., Steinmetz, M., Benoist, C. O., and Mathis, D. J.: Inbred and wild mice carry identical deletions in Eα Mhe genes. EMBO J., in press, 1985
Ferreira, A., Nussenzweig, V., and Gigli, L: Structural and functional differences between the H-2 controlled Ss and Slp proteins. J. Exp. Med. 148: 1186–1197, 1978
Ferreira, A., Michaelson, J., and Nussenzweig, V.: H-2-controlled polymorphism of the γ chain of Slp (sex-limited protein). Immunogenetics 11: 491–497, 1980
Figueroa, F., Golubie, M., Nizetić, D., and Klein, J.: Evolution of mouse Mhc genes borne by t chromosomes. Proc. Natl. Acad. Sci. U.S.A., in press, 1985
Gorman, J., Jackson, R., Desantola, J., Shreffler, D., and Atkinson, J.: Development of a hemolytic assay for mouse C2 and determinants of its genetic control. J. Immunol. 125: 344–351, 1980
Hammerberg, C. and Klein, J.: Linkage disequilibrium between H-2 and t complexes in chromosome 17 of the mouse. Nature 258: 296–299, 1975
Hansen, T. H., Krasteff, T. N., and Shreffler, D. C.: Quantitative variation in the expression of the mouse serum antigen Ss and its sex-limited allotype Slp. Biochem. Genet. 12: 281–293, 1974
Klein, J.: A case of no sex-limitation of Slp in the murine H-2 complex. Immunogenetics 2: 297–299, 1975
Klein, J., Sipos, P., and Figueroa, F.: Polymorphism of t-complex genes in European wild mice. Genet. Res. (Comb.) 14: 39–46, 1984
Klein, J., Nizetić, D., Golubić, D., Dembić, Z., and Figueroa, F.: Evolution of H-2 genes on t chromosomes. In H. Vogel and B. Pernis (ed.): in press, 1985
Lévi-Strauss, M., Tosi, M., Steinmetz, M., Klein, J., and Meo, T.: Multiple duplications of complement C4 gene correlate with H-2 controlled testosterone-independent expression of its sex-limited isoform, C4-Slp. Proc. Natl. Acad. Sci. U.S.A., in press, 1985a
Lévi-Strauss, M., Georgatsou, E., Tosi, M., and Meo, T.: Gene-specific probes demonstrate selective duplications of the C4-Slp gene in the H-2S alleles associated with a testosterone-independent expression of this isotype. Immunogenetics, in press, 1985b
Natsuume-Sakai, S., Kaidoh, Z., Nonaka, M., and Takahashi, M.: Structural polymorphism of murine C4 and its linkage to H-2. J. Immunol. 124: 2714–2720, 1980
Nizetić, D., Figueroa, F., and Klein, J.: Evolutionary relationships between the t and H-2 haplotypes in the house mouse. Immunogenetics 19: 311–320, 1984
Parker, K. L., Atkinson, J. P., Roos, M. H., and Shreffler, D. C.: Genetic and structural characterization of H-2-controlled allelic forms of murine C4. Immunogenetics 11: 55–63, 1980
Passmore, H. C. and Shreffler, D. C.: A sex-limited serum protein variant in the mouse: Inheritance and association with the H-2 region. Biochem Genet. 4: 351–365, 1970
Passmore, H. C. and Shreffler, D. C.: A sex-limited serum protein variant in the mouse: Hormonal control of phenotypic expression. Biochem. Genet. 5: 201–209, 1971
Roos, M. H. and Démant, P.: Murine complement factor B (BF): Sexual dimorphism and H-2-linked polymorphism. Immunogenetics 15: 23–30, 1982
Roos, M. H., Atkinson, J. P., and Shreffler, D. C.: Molecular characterization of the Ss and Slp (C4) proteins of the mouse H-2 complex: Subunit composition, chain size polymorphism and an intracellular (pro-Ss) precursor. J. Immunol. 121: 1106–1115, 1978
Sackstein, R., Roos, M. H., Demant, P., and Colten, H. R.: Subdivision of the S region of the mouse histocompatibility complex by identification of genomic polymorphisms of the class III genes. Immunogenetics 20: 321–330, 1984
Shreffler, D. C.: Mhc-linked complement components. In P. Parham and J. Strominger (eds.): Histocompatibility Antigens, Structure and Function: Receptor and Recognition, 1982
Shreffler, D. C. and Owen, R. D.: A serologically detected variant in mouse serum: Inheritance and association with the histocompatibility-2 locus. Genetics 48: 9–25, 1963
Silver, L. M. and Artzt, K.: Recombination suppression of mouse t-haplotypes due to chromatin mismatching. Nature 290: 68–70, 1981
Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503–517, 1975
Sturm, S., Figueroa, F., and Klein, J.z: The relationship between t and H-2 complexes in wild mice. I. The H-2 haplotypes of 20 t-bearing strains. Genet. Res. (Camb.) 40: 73–88, 1982
Tosi, M., Levi-Strauss, M., Duponchel, C., and Meo, T.: Sequence heterogeneity of murine cDNA clones related to the C4 and C4-Slp isoforms of the fourth complement component. Philos. Trans. R. Soc. Lond. Biol. 306: 386–394, 1984
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Golubić, M., Figueroa, F., Tosi, M. et al. Restriction fragment length polymorphism of c4 genes in mice with t chromosomes. Immunogenetics 21, 247–256 (1985). https://doi.org/10.1007/BF00375377
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DOI: https://doi.org/10.1007/BF00375377