Abstract
The debriding activity of the protease complex produced from the hepatopancreas of the king crab Paralithodes camtschatica was evaluated in vivo. The results clearly showed that the crab preparation had a high debriding ability. Individual proteases were isolated chromatographically from the crab complex to compare their debriding activities. All the purified proteases were active in vivo. The most active component of the complex was the isozyme C of crab collagenolytic protease, a member of the chymotrypsin-like protease class.
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References
Artyukov AA, Sakharov IY (1991) Method of collagenase production. USSR Patent N4806987
Baramova EN, Shannon JD, Bjarnason JB, Fox JW (1990) Identification of the cleavage sites by a hemorrhagic metalloproteinase in type IY collagen. Matrix 10: 91–97
Brentano L, Gravine DJ (1967) A method for the quantitation of bacteria in burn wound. Appl Microbiol 15: 670–671
Campbell D, Hellgren L, Karlstam B, Vincent J (1987) Debriding ability of a multi-enzyme preparation isolated from Antarctic krill (Euphausia superba). Experimentia 43: 578–579
Hellgren L, Vincent J (1989) Debriding properties of krill enzymes in necrotic leg ulcers. Arch Dermatol 127: 1006
Hellgren L, Mohr V, Vincent J (1986) Proteases of Antarctic krill — a new system for effective enzymatic debridement of necrotic ulcerations. Experimentia 42: 403–404
Mandl I, MacLennan JD, Howes EL (1953) Isolation and characterization of proteinase and collagenase from Clostridium histolyticum. J Clin Invest 32: 1323–1329
Sakharov IY, Litvin FE (1990) Stability of serine collagenolytic protease A from hepatopancreas of crab Paralithodes camtschatica. Comp Biochem Physiol [B] 97: 407–410
Sakharov IY, Litvin FE (1992) Substrate specificity of collagenolytic proteases from hepatopancreas of the king crab. Biochemistry (USSR) 57: 61–69
Sakharov IY, Litvin FE, Artyukov AA, Kofanova NN (1989) Purification and characterization of collagenolytic protease A from hepatopancreas of Paralithodes camtschatica. Biochemistry (USSR) 53: 1588–1591
Sakharov IY, Dzunkovskaya AV, Zakharova NV, Artyukov AA (1991) Pancreatic elastase — new serine protease isolated from hepatopancreas of the king crab (Abstr). In: Biologically active substances of hydrobionts — new medicine and techinical preparations. Vladivostok, 8
Sakharov IY, Litvin FE, Artyukov AA (1993) Purification and characterization of two serine collagenolytic proteases from crab Paralithodes camtschatica. Arch Biochem Biophys (in press)
Sakharov IY, Litvin FE, Artyukov AA (1992) Physico-chemical properties of collagenolytic protease C from the king crab. Biochemistry (USSR) 57: 40–45
Stuwe U (1983) Enzymatische Wundreinigung. Fortschr Med 41: 1883–1888
Wokalek H, Niedner R (1984) Enzymatische Wundreinigung. Extr Med Pract 5: 35–42
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Sakharov, I.Y., Glyanzev, S.P., Litvin, F.E. et al. Potent debriding ability of collagenolytic protease isolated from the hepatopancreas of the king crab Paralithodes camtschatica . Arch Dermatol Res 285, 32–35 (1993). https://doi.org/10.1007/BF00370820
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DOI: https://doi.org/10.1007/BF00370820