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Elektronenmikroskopisch-autoradiographische Untersuchungen über die Eiweiß-Synthese in der Brunnerschen Drüse der Maus

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Summary

Swiss Mice weighing 20 g each received a single intraperitoneal injection of 800 μC Leucine-3-H and were sacrified at intervals from 5 minutes until an end time of 240 minutes after giving the labelled leucine.

The incorporation of leucine in the proteins of the glands of Brunner was then observed by electronmicroscopic-autoradiography. The following results were obtained from morphologic and semiquantitative observations:

The rough endoplasmic reticulum plays a prime rôle in the synthesis of these proteins. The labelled proteins are then displaced to the Golgi vesicles and Golgi cisternae (15–30 minutes after the injection of labelled leucine). The main part of the radioactivity appears in the mature granules after the injection, while some radioactivity appears in proteins of the gland cells.

The possible functions of the Golgi field are discussed. A small labelling of the nucleus and the mitochondria is observed. Therefore some synthetic activity of these organellae is supposed. The possibilities of error and methodic difficulties are discussed.

Zusammenfassung

20 g schwere Mäuse erhielten eine einmalige Dosis von 800 μC 3H-Leucin i.p. injiziert und wurden in Intervallen von 15–240 min getötet. Um zu semiquantitativen Aussagen zu gelangen, wurden die Volumenrelationen der Zellkompartimente morphometrisch bestimmt, die Silberkornfilamente an den elektronenmikroskopischen Autoradiogrammen ausgezählt und die relativen spezifischen Lokalisationen von 3H-Leucin errechnet.

Bei der Sekretsynthese lassen sich drei Phasen feststellen: eine ergastoplasmatische, eine Golgi- und eine Ausschleusungsphase. Diskutiert wird der Transportmechanismus der neugebildeten Proteine vom Ergastoplasma zum Golgi-Feld und die Funktion des Golgi-Komplexes bei der Eiweißsynthese. Als mögliche Funktionen des Golgikomplexes werden Kondensation der Sekretproteine, Koppelung der Proteine mit Kohlenhydraten sowie eine mögliche Rolle als Schaltstelle und als Membrandepot herausgestellt. Die regelmäßige Kernmarkierung läßt eine Proteinsynthese im Kern vermuten. Eine mitochondriale Proteinsynthese wird zur Diskussion gestellt. Auf einige Fehlerquellen bei der Beurteilung elektronenmikroskopischer Autoradiographie wird hingewiesen.

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Authors and Affiliations

  1. Ludwig-Aschoff-Haus, dem Pathologischen Institut der Universität Freiburg i. Br., Deutschland

    H. P. Rohr, J. Schmalbeck & A. Feldhege

Authors
  1. H. P. Rohr
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  2. J. Schmalbeck
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  3. A. Feldhege
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Additional information

Ausgeführt mit Unterstützung durch die Deutsche Forschungsgemeinschaft und die Sandoz A.G. (Basel).

Wesentliche Teile der vorliegenden Arbeit werden von Jürgen Schmalbeck als Dissertation der Medizinischen Fakultät der Universität Freiburg i. Br. vorgelegt.

Die vorliegende Arbeit zeigt, daß auch in der Brunnerschen Drüse der Maus die Sekretproduktion an Zellstrukturen gebunden ist, die als Stationen des Syntheseweges von Sekretproteinen aufgefaßt werden.

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Rohr, H.P., Schmalbeck, J. & Feldhege, A. Elektronenmikroskopisch-autoradiographische Untersuchungen über die Eiweiß-Synthese in der Brunnerschen Drüse der Maus. Zeitschrift für Zellforschung 80, 183–204 (1967). https://doi.org/10.1007/BF00337455

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