Summary
A cosmid library was constructed by inserting genomic DNA isolated from a wild-type aflatoxin-producing strain of Aspergillus parasiticus (SU-1) into a cosmid vector containing an homologous nitrate reductase (niaD) gene as a selectable marker. One cosmid was isolated which complemented an aflatoxin-deficient, nitrate-nonutilizing mutant strain, A. parasiticus B62 (nor-1, niaD), to aflatoxin production. Deletion and complementation analyses showed that, a 1.7 kb BglII-SphI DNA fragment isolated form this cosmid was responsible for renewed aflatoxin production. Northern hybridization analyses revealed that the major RNA transcribed from this DNA fragment, was 1.4 kilonucleotides in size. Genetic complementation, proved to be a useful strategy for cloning a gene associated with aflatoxin biosynthesis in A. parasiticus.
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References
Arseculeratne SN, De Silva LM, Wijesundera S, Bandunatha CHSR (1969) Appl Microbiol 18:88–94
Anderson JA, Chung CH (1990) Mycopathologica 110:31–35
Anderson JA, Chung CH, Cho S-H (1990) Mycopathologia 111:39–45
Bennett JW (1979) J Gen Microbiol 113:127–136
Bennett JW, Lee LS, Shoss SM, Boudreaux GH (1980) Appl Environ Microbiol 39:835–839
Bhatnagar D, Cleveland TE, Lillchoj EB (1989) Mycopathologia 107:75–83
Bhatnagar D, Cleveland TE (1990) FASEB J 4:2164–2167
Bhatnagar D, Cleveland TE, Kingston DGI (1991) Biochemistry 30:4343–4350
Buchanan RL, Jones SB, Gerasimowicz WV, Zaika LL, Stahl HG, Ocker LA (1987) Appl Environ Microbiol 53:1224–1231
Chuturgoon AA, Dutton MF, Berry RK (1990) Biochem Biophys Res Commun 166:38–42
Cihlar R, Sypherd PS (1980) Nucleic Acids Res 8:793–804
Cleveland TE, Bhatnagar D (1990) Can J Microbiol 36:1–5
Cleveland TE, Lax AR, Lee LS, Bhatnagar D (1987) Appl Environ Microbiol 53:1171–1713
Donkersloot JA, Mateles RI Yang SS (1972) Biochem Biophys Res Commun 47:1051–1055
Dutton MF (1988) Microbiol Rev 52:274–295
Dvorackova I (1990) Aflatoxins and human health. CRC Press, Florida, USA
Helathcote JG, Hibbert JR (1978) Aflatoxins: chemical and biological aspects. Elsevier Scientific Publishing Company, The Netherlands
Hohn B, Collins J (1980) Gene 11:291–298
Horng JS, Chang P-K, Pestka JJ, Linz JE (1990) Mol Gen Genet 224:294–296
Hsieh DPH, Lin MT, Yao RC (1973) Biochem Biophys Res Commun 52:992–997
Hsieh DPH, Wan CC, Billington JA (1989) Mycopathology 107:121–126
Jeenah MS, Sutton MF (1983) Biochem Biophys Res Commun 116:1114–1118
Lee LS, Bennett JW, Goldblatt LA, Lundin RE (1970) J Am Oil Chem Soc 48:93–94
Lee LS, Bennett JW, Cucullu AF, Stanley JB (1975) J Agri Food Chem 23:1132–1134
Lee LS, Bennett JW, Cucullu AF, Ory RL (1976) J Agri Food Chem 24:1167–1170
Maniatis T, Fritsch EF, Sambrook J (1987) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Maramatsu M (1973) Methods Cell Biol 7:23–51
Singh R, Hsieh DPH (1977) Arch Biochem Biophys 178:285–292
Vollmer SJ, Yanofsky C (1986) Proc Natl Acad Sci USA 83:4869–4873
Wan NC, Hsieh DPH (1980) Appl Environ Micro 39:109–112
Yabe K, Ando Y, Hashimoto J, Hamasaki T (1989) Appl Environ Microbiol 55:2171–2177
Yabe K, Nakamura Y, Nakajima H, Ando Y, Hamasaki T (1991) Appl Environ Microbiol 57:1340–1345
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Chang, PK., Skory, C.D. & Linz, J.E. Cloning of a gene associated with aflatoxin B1 biosynthesis in Aspergillus parasiticus . Curr Genet 21, 231–233 (1992). https://doi.org/10.1007/BF00336846
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DOI: https://doi.org/10.1007/BF00336846