Abstract
The problem to be examined is the extent to which induced changes in metabolism influence the mutagenic activity of several N-nitroso-compounds in the host-mediated assay. After pretreatment with carbon tetrachloride it is impossible to induce mutations with dimethylnitrosamine in the host-mediated assay system. Pretreatment with phenobarbital does not influence the mutagenic activity of this compound. Methyl-nitroso-nitroguanidine has mutagenic effects only when applied parenterally, but not when given orally. Methyl-nitrosourea induces mutations when injected subcutaneously and when given orally. These results may be explained by differences of absorption.
Zusammenfassung
Es wird untersucht, inwieweit induzierte Veränderungen des Metabolismus die mutagene Wirksamkeit einiger N-Nitrosoverbindungen im host-mediated assay beeinflussen können. Eine Vorbehandlung der Maus mit Tetrachlorkohlenstoff macht es unmöglich, mit Dimethylnitrosamin im host-mediated assay Mutationen auszulösen. Die Vorbehandlung mit Phenobarbital hat keinen Einfluß auf die mutagene Wirkung dieser Verbindung. Methyl-nitroso-nitroguanidin wirkt nur nach parenteraler, nicht nach oraler Applikation, mutagen. Methylnitrosoharnstoff induziert Mutationen bei subcutaner Injektion und oraler Gabe. Die Verschiedenheiten werden auf eine unterschiedliche Resorption zurückgeführt.
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This work was sponsored by the Deutsche Forschungsgemeinschaft.
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Propping, P., Buselmaier, W. The influence of metabolism on mutagenic activity in the host-mediated assay. Arch. Toxikol. 28, 129–134 (1971). https://doi.org/10.1007/BF00332388
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DOI: https://doi.org/10.1007/BF00332388