Summary
Rice glutelin cDNA clones were isolated from a rice cDNA library constructed from immature endosperm. The isolated glutelin cDNA clones could be divided into two classes by restriction site polymorphism and sequence. They encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. The coding regions were 95% homologous to each other at the nucleotide and amino acid levels. Southern hybridization analysis revealed that there were four or five copies of glutelin genes per haploid rice genome.
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References
Bendich AJ, Anderson RS, Ward BL (1980) Plant DNA: Long, pure and simple. In: Leaver CJ (ed) Genome organization and expression in plants. Plenum Press, New York, pp 31–33
Chou PY, Fasman GD (1980) Prediction of protein secondary structure. Adv Enzymol 47:45–148
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J (1986) mRNA transcripts of several genes are polyadenylated at multiple sites in vivo. Nucleic Acids Res 14:2229–2240
Domoney C, Casey R (1985) Measurement of gene number for seed storage proteins in Pisum. Nucleic Acids Res 13:687–699
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Grunstein M, Hogness D (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain specific gene. Proc Natl Acad Sci USA 72:3961–3965
Gubler U, Hoffman B (1983) A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
Kozak M (1984) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res 12:857–872
Krishnan HB, Okita TW (1986) Structural relationship among the rice glutelin polypeptides. Plant Physiol 81:105–132
Kyte J, Doolittle RF (1982) A simple method for displaying the character of a protein. J Mol Biol 157:105–132
Lycett GW, Delauney AJ, Croy RRD (1983) Are plant genes different? FEBS Lett 153:43–46
Lycett GW, Croy RRD, Shirsat AH, Boulter D (1984) The complete nucleotide sequence of a legumine gene from pea (Pisum sativum L.). Nucleic Acids Res 12:4493–4506
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, New York
Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
Mclauchlan J, Gaffney D, Whitton JL, Clements JB (1985) The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA termini. Nucleic Acids Res 13:1347–1368
Negoro T, Momma T, Fakazawa C (1985) A cDNA clone encoding a glycinin A1a subunit precursor of soybean. Nucleic Acids Res 13:6719–6731
Oono K, Sugiura M (1980) Heterogeneity of the ribosomal RNA gene clusters in rice. Chromosoma 76:85–89
Osborne TB (1909) The Vegetable Proteins, Longmans, Green and Co, London
Robert LS, Nozzolillo C, Altosaar I (1985) Homology between rice glutelin and oat 12S globulin. Biochim Biophy Acta 829:19–26
Scallon B, Thanh VH, Floener LA, Nielsen NC (1985) Identification and characterization of DNA clones encoding group-II glycinin subunits. Theor Appl Genet 70:510–519
Simon AF, Tenbarge KM, Scofield SR, Finkelstein RR, Crouch ML (1985) Nucleotide sequence of a cDNA clone of Brassica napus 12S storage protein shows homology with legumin from Pisum sativum. Plant Mol Biol 5:191–201
Southern EM (1975) Detection of specific sequences among DNA fragments separated by electrophoresis. J Mol Biol 98:503–517
Staswick PE, Hermodson MA, NIelsen NC (1984) Identification of the cysteines which link the acidic and basic components of the glycinin subunits. J Biol Chem 259:13431–13435
Takaiwa F, Sugiura M (1982) The complete nucleotide sequence of a 23S rRNA gene from tobacco chloroplasts. Eur J Biochem 124:13–19
Takaiwa F, Kikuchi S, Oono K (1986) The structure of rice storage protein glutelin precursor deduced from cDNA. FEBS Lett 206:33–35
Von Heijne G (1983) Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem 133:17–21
Walburg G, Larkins BA (1986) Isolation and characterization of cDNAs encoding oat 12S globulin mRNAs. Plant Mol Biol 6:161–169
Wen T-N, Luthe D (1985) Structural relationship among the rice glutelin polypeptides. Plant Physiol 81:748–753
Yamataga H, Sugimoto T, Tanaka K, Kasai Z (1982) Biosynthesis of storage proteins in developing rice seeds. Plant Physiol 70:1094–1100
Zhao W-M, Gatehouse JA, Boulter D (1983) The purification and partial amino acid sequence of a polypeptide from the glutelin fraction of rice grains: homology to legumin. FEBS Lett 162:96–102
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Communicated by H. Saedler
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Takaiwa, F., Kikuchi, S. & Oono, K. A rice glutelin gene family — a major type of glutelin mRNAs can be divided into two classes. Mole Gen Genet 208, 15–22 (1987). https://doi.org/10.1007/BF00330416
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DOI: https://doi.org/10.1007/BF00330416