Summary
In situ hybridization procedure with a 32P-labelled synthetic oligonucleotide probe was used to detect corticotropin-releasing factor-encoding messenger RNA (CRF mRNA) in the hypothalamus of the white sucker, Catostomus commersoni. Adjacent sections were immunostained by a sucker CRF-specific antiserum. CRF mRNA-containing neurons were identified by autoradiography in the magnocellular and parvocellular subdivisions of the preoptic nucleus (PON). Many of these neurons were also immunostained by sucker antiserum, showing the same distribution patterns. These results confirm the presence of CRF mRNA and CRF peptide in the white sucker hypothalamus and support the view that the magnocellular and parvocellular neurons of the PON may be involved in the control of adrenocorticotropic hormone secretion from the pituitary in the white sucker.
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References
Fellman D, Bugnon C, Bressen JL, Gouget A, Caldol J, Clavequin MC, Hadiyiasemis M (1984) The CRF neuron: immunocytological study. Peptides [Suppl] 5:19–33
Fryer J, Lederis K, Rivier J (1983) Urotensin I, a CRF-like neuropeptide, stimulates ACTH release from the teleost pituitary. Endocrinology 113:2308–2310
Fryer JN, Lederis K, Rivier J (1985) ACTH-releasing activity of urotensin I and ovine CRF: Interactions with arginine vasotocin, isotocin and arginine vasopressin. Reg Peptides 11:11–15
Furutani Y, Morimoto Y, Shibahara S, Noda M, Takahashi H, Hirose T, Asai M, Inayama S, Hayashida H, Miyata T, Numa S (1983) Cloning and sequence analysis of cDNA for ovine corticotropin-releasing factor precursor. Nature 301:537–540
Ichikawa T, Ishida I, Ohsako S, Deguchi T (1988) In situ hybridization demonstrating coexpression of urotensin II, II-α, and II-β in the caudal neurosecretory neurons of the carp Cyprinus carpio Gen Comp Endocrinol 71:493–501
Jingami H, Mizuno N, Takahashi H, Shibahara S, Furutani Y, Imura H, Numa S (1985) Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor gene precursor. FEBS Lett 191:63–66
Kovács KJ, Mezey É (1987) Dexamethasone inhibits corticotropin-releasing factor gene expression in the rat paraventricular nucleus. Neuroendocrinology 46:365–368
Lederis K, Letter A, McMaster D, Ichikawa T, MacCannell KL, Kobayashi Y, Rivier J, Rivier C, Vale W, Fryer J (1983) Isolation, analysis of structure, synthesis, and biological actions of urotensin I neuropeptides. Can J Biochem Cell Biol 61:602–614
Lederis K, Ko D, Rivier J, Melchiorri P, Negri L (1987) Specificity and sensitivity of antisera produced against non-conjugated urotensin I and related peptides. Proc West Pharmacol Soc 30:187–189
Maxam A, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
McMaster D, Lederis K (1988) Urotensin I-and CRF-like peptides in Catostomus commersoni brain and pituitary—HPLC and RIA characterization. Peptides 9:1043–1048
McMaster D, Rivier J, Lederis K (1988) Isolation, amino acid sequence and synthesis of urotensin I form from Hyppoglossoides elassodon. In: Shibata T, Sakakibara S (eds) Peptide chemistry 1987. Protein Research Foundation, Osaka, pp 145–148
Okawara Y, Morley SD, Burzio LO, Zwiers H, Lederis K, Richter D (1988) Cloning and sequence analysis of cDNA for corticotropin-releasing factor from the teleost fish Catostomus commersoni. Proc Natl Acad Sci USA 85:8439–8443
Olivereau M, Olivereau J (1988) Localization of CRF-like immunoreactivity in the brain and pituitary of teleost fish. Peptides 9:13–21
Olivereau M, Ollevier F, Vandesande F, Verdonck W (1984) Immunocytochemical identification of CRF-like and SRIF-like peptides in the brain and the pituitary of cyprinid fish. Cell Tissue Res 237:379–382
Rentrop M, Knapp B, Winter H, Schweizer J (1986) Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin cDNA to frozed sections under varying fixation and pretreatment conditions. Histochem J 18:271–276
Rivier C, Rivier J, Lederis K, Vale W (1983) In vitro and in vivo ACTH-releasing activity of ovine CRF, sauvagine and urotensin I. Reg Peptides 5:139–143
Shibahara S, Morimoto Y, Furutani Y, Notake M, Takahashi H, Shimizu S, Horikawa S, Numa S (1983) Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene. EMBO J 2:775–779
Spiess J, Rivier J, Rivier C, Vale W (1981) Primary structure of corticotropin-releasing factor from ovine hypothalamus. Proc Natl Acad Sci USA 78:6517–6521
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antiperoxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Thompson RC, Seasholtz AF, Herbert E (1987) Rat corticotropin-releasing hormone gene: Sequence and tissue-specific expression. Mol Endocrinol 1:363–370
Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates the secretion of corticotropin and β-endorphin. Science 213:1394–1397
Valentino KL, Eberwine JH, Barchas JD (1987) In situ hybridization. Application to neurobiology. Oxford University Press, New York
Young WS III, Mezey É, Siegel RE (1986) Quantative in situ hybridization histochemistry reveals increased level of corticotropin-releasing factor mRNA after adrenalectomy in rats. Neurosci Lett 70:198–203
Yulis CR, Lederis K (1986) The distribution of ‘extraurophyseal’ urotensin I-immunoreactivity in the central nervous system of Catostomus commersoni after urophysectomy. Neurosci Lett 70:75–80
Yulis CR, Lederis K (1987) Co-localization of the immunoreactivities of corticotropin-releasing factor and arginine vasotocin in the brain and pituitary system of the teleost Catostomus commersoni. Cell Tissue Res 247:267–273
Yulis CR, Lederis K, Wong K-L, Fisher AWF (1987) Localization of urotensin I- and corticotropin-releasing factor-like immunoreactivity in the central nervous system of Catostomus commersoni. Peptides 7:79–86
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Okawara, Y., Ko, D., Morley, S.D. et al. In situ hybridization of corticotropin-releasing factor-encoding messenger RNA in the hypothalamus of the white sucker, Catostomus commersoni . Cell Tissue Res 267, 545–549 (1992). https://doi.org/10.1007/BF00319377
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DOI: https://doi.org/10.1007/BF00319377