Abstract
In the present immunohistochemical study, we investigated the characteristics of altered neurites in the frontal cortex of 10 Alzheimer's disease (AD) brains and 15 age-matched non-demented control brains. In both AD and control cases, the altered neurites in coronas of the classical plaques (CP) were frequently immunostained by antibodies to growth-promoting factors, N and C termini of amyloid precursor protein (APP), GAP43, collagen IV, laminin and the integrin receptor VLA6. The altered neurites in CP coronas in AD but not in controls were also immunostained by antibodies against normally and abnormally phosphorylated tau. Immunolabeling for microtubule-associated protein 2 was not found in CP from either group. Extensive neuropil threads (NT) and many neurofibrillary tangles (NFT), immunostained with tau and Alz50 antibodies, were present in AD neocortex but not seen in control cases. NT and NFT could not be stained by antibodies to the N termini of APP, GAP43, collagen IV, laminin and VLA6. Our findings indicate that in AD cases altered neurites in CP are undergoing both an aberrant sprouting process and a degenerating process. These altered neurites are probably of axon origin. NT and NFT may represent destructive changes. The presence of amyloid plaques, but absence of tau-related cytoskeletal pathology, in non-demented cases suggests that β/A4 peptide is necessary but not sufficient to induce neurofibrillary pathology.
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Abraham CR, Selkoe DJ, Potter H (1988) Immunochemical identification of the serine protease inhibitor α1-antichymotrypsin in the brain amyloid deposits of Alzheimer's disease. Cell 52:487–501
Akiyama H, Kawamata T, Dedhar S, McGeer PL (1991) Immunohistochemical localization of vitronectin, its receptor and beta-3 integrin in Alzheimer brain tissue. J Neuroimmunol 32: 19–28
Arai H, Lee VM, Otvos L Jr, Greenberg BD, Lowery DE, Sharma SK, Schmidt ML, Trojanowski JQ (1990) Defined neurofilament, tau, and beta-amyloid precursor protein epitopes distinguish Alzheimer from non-Alzheimer senile plaques. Proc Natl Acad Sci USA 87:2249–2253
Araki W, Kitaguchi N, Tokushima Y, Ishii K, Aratake H, Shimohama S, Nakamura S, Kimura J (1991) Trophic effect of beta amyloid precursor protein on cerebral cortical neurons in culture. Biochem Biophys Res Commun 181:265–271
Barcikowska M, Wisniewski HM, Bancher C, Grundke-Iqbal I (1989) About the presence of paired helical filaments in dystrophic neurites participating in the plaque formation. Acta Neuropathol 78:225–231
Bauer J, Strauss S, Schreiter-Gasser U, Ganter U, Schlegel P, Witt I, Yolk B, Berger M (1991) Interleukin-6 and α-2-macroglobulin indicate an acute-phase state in Alzheimer's disease cortices. FEBS Lett 285:111–114
Benzing WC, Ikonomovic MD, Brady DR,Mufson EJ, Armstrong D (1993) Evidence that transmitter-containing dystrophic neurites precede paired helical filament and Alz-50 formation within senile plaques in the amygdala of nondemented elderly and patients with Alzheimer's disease. J Comp Neurol 334:176–191
Berkenbosch F, Biewenga J, Brouns M, Rozemuller JM, Strijbos P, van Dam AM (1992) Cytokines and inflammatory proteins in Alzheimer's disease. Res Immunol 143:657–663
Bondareff W, Mountjoy CQ, Roth M (1982) Loss of neurons or origin of the adrenergic projection to cerebral cortex (nucleus locus coeruleus) in senile dementia. Neurology 32:164–168
Bouman L (1934) Senile plaques. Brain 57:128–142
Braak H, Braak E (1988) Neuropil threads occur in dendrites of tangle-bearing nerve cells. Neuropathol Appl Neurobiol 14: 39–44
Braak H, Braak E (1993) Entorhinal-hippocampal interaction in mnestic disorders. Hippocampus 3:239–246
Braak H, Braak E, Grundke-Iqbal I, Iqbal K (1986) Occurrence of neuropil threads in the senile human brain and in Alzheimer's disease: a third location of paired helical filaments outside of neurofibrillary tangles and neuritic plaques. Neurosci Lett 65:351–355
Breen KC (1992) APP-collagen interaction is mediated by a heparin bridge mechanism. Mol Chem Neuropathol 16:109–121
Breen KC, Bruce M, Anderton BH (1991) Beta amyloid precursor protein mediates neuronal cell-cell and cell-surface adhesion. J Neurosci Res 28:90–100
Caputo CB, Evangelista Sobel IR, Scott CW, Brunner WF, Barth PT, Blowers DP (1992) Association of the carboxyterminus of β-amyloid protein precursor with Alzheimer paired helical filaments. Biochem Biophys Res Commun 185:1034–1040
Cras P, Kawai M, Lowery D, Gonzalez-DeWhitt P, Greenberg B, Perry G (1991) Senile plaque neurites in Alzheimer disease accumulate amyloid precursor protein. Proc Natl Acad Sci USA 88:7552–7556
Curcio CA, Kemper T (1984) Nucleus raphe dorsalis in dementia of the Alzheimer type: neurofibrillary changes and neuronal packing density. J Neuropathol Exp Neurol 43:359–368
De Camilli P, Miller P, Navone F, Theurkauf W, Vallee RB (1984) Distribution of microtubule-associated protein 2 (MAP2) in the nervous system of the rat studied by immunofluorescence. Neuroscience 11:819–846
Dickson DW, Farlo J, Davies P, Crystal H, Fuld P, Yen SH (1988) Alzheimer's disease: a double-labeling immunohisto-chemical study of senile plaques. Am J Pathol 132:86–101
Dickson DW, Fishman A, Mattiace LA, Yen SH (1991) An epitope shared with the carboxy terminus of amyloid precursor protein (APP) is associated with neuronal cytoskeleton and neurofibrillary tangles (NFT). J Neuropathol Exp Neurol 50: 317
Eikelenboom P, Hack CE, Rozemuller JM, Stam FC (1989) Complement activation in amyloid plaques in Alzheimer's dementia. Virchows Arch [B] 56:259–262
Eikelenboom P, Zhan SS, Kamphorst W, van der Valk P, Rozemuller JM (1994) Cellular and substrate adhesion molecules (integrins) and their ligands in cerebral amyloid deposits in Alzheimer's disease, Virchows Arch 424:421–427
Fischer O (1907) Miliare Nekrosen mit drusigen Wucherungen der Neurofibrillen, eine regelmässige Veränderung der Hirnrinde bei seniler Demenz. Monatsschr Psychiatr Neurol 22: 361–372
Gispen WH, Boonstra J, De Graan PNE (1990) B-50/GAP43 in neuronal development and repair. Res Neurol Neurosci 1: 237–244
Goedert M, Sisodia SS, Price DL (1991) Neurofibrillary tangles and β-amyloid deposits in Alzheimer's disease. Curr Opin Neurobiol 1:441–447
Griffin WST, Stanley LC, Ling C, White L, MacLeod V, Perrot LJ, White CL, Araoz C (1989) Brain interleukin 1 and S-100 immunoreactivity are elevated in Down syndrome and Alzheimer disease. Proc Natl Acad Sci USA 86:7611–7615
Iqbal K, Grundke-Iqbal I (1991) Ubiquitination and abnormal phosphorylation of PHFs in Alzheimer's disease. Mol Neurobiol 5:399–410
Kalaria RN, Perry G (1993) Amyloid P component and other acute-phase proteins associated with cerebellar Aβ-deposits in Alzheimer's disease. Brain Res 631:151–155
Kosik KS (1992) Alzheimer's disease: a cell biological perspective. Science 256:780–783
Kosik KS, Joachim CL, Selkoe DJ (1986) Microtubule-associated protein, tau, is a major antigenic component of paired helical filaments in Alzheimer's disease. Proc Natl Acad Sci USA 83:4044–4048
Lee VM-Y, Trojanowski JQ (1992) The disordered neuronal cytoskeleton in Alzheimer's disease. Curr Opin Neurobiol 2: 653–656
Leigh PN, Probst A, Dale GE, Power DP, Brion J-P, Dodson A, Anderton BH (1989) New aspects of the pathology of neurodegenerative disorders as revealed by ubiquitin antibodies. Acta Neuropathol 79:61–72
Masliah E, Terry RD, Alford M, DeTeresa R, Hansen LA (1991) Cortical and subcortical patterns of synaptophysin-like immunoreactivity in Alzheimer's disease. Am J Pathol 138:235–246
Masliah E, Mallory M, Hansen L, Alford M, DeTeresa R, Terry R, Baudier J, Saitoh T (1992) Localization of amyloid precursor protein in GAP43-immunoreactive aberrant sprouting neurites in Alzheimer's disease. Brain Res 574:312–316
Masliah E, Ellisman M, Carragher B (1992) Three-dimensional analysis of the relationship between synaptic pathology and neuropil threads in Alzheimer disease. J Neuropathol Exp Neurol 51:404–414
Masliah E, Mallory M, Hansen L, Alford M, DeTeresa R, Terry R (1993) An antibody against phosphorylated neurofilaments identifies a subset of damaged association axons in Alzheimer's disease. Am J Pathol 142:871–882
Masure S, Opdenakker G (1989) Cytokine-mediated proteolysis in tissue remodelling. Experientia 45:542–549
Narindrasorasak S, Lowery DE, Altman RA, Gonzalez-DeWhitt PA, Greenberg BD, Kisilevsky R (1992) Characterization of high-affinity binding between laminin and Alzheimer's disease amyloid precursor proteins Lab Invest 67:643–652
Perry G, Kawai M, Tabaton M, Onorato M, Mulvill P, Richey P, Morandi A, ConnoLLy JA, Gambetti P (1990) Neuropil threads of Alzheimer's disease show a marked alteration of the normal cytoskeleton. J Neurosci 11:1748–1755
Probst A, Brunnschweiler H, Lautenschlager C, Ulrich J (1987) A special type of senile plaque, possibly an initial stage. Acta Neuropathol (Berl) 74:133–141
Rozemuller JM, Eikelenboom P, Pals ST, Stam FC (1989) Microglial cells around amyloid plaques in Alzheimer's disease express leucocyte adhesion molecules of the LFA-1 family. Neurosci Lett 101:288–292
Rozemuller JM, Eikelenboom P, Stam FC, Beyreuther K, Masters CL (1989) A4 protein in Alzheimer's disease: primary and secondary cellular events in extracellular amyloid deposition. J Neuropathol Exp Neurol 48:647–663
Rozemuller JM, Abink JJ, Kamp AM, Stam FC, Hack CE, Eikelenboom P (1991) Distribution pattern and functional state of α1 antichymotrypsin in plaques and vascular amyloid. Acta Neuropathol 82:200–207
Rozemuller JM, Roos RAC, Bots GTAM, Kamphorst W, Eikelenboom P, Van Nostrand WE (1993) Distribution of β/A4 protein and amyloid precursor protein in hereditary cerebral hemorrhage with amyloidosis-Dutch type and Alzheimer's disease. Am J Pathol 142:1449–1457
Sanes JR (1989) Extracellular matrix molecules that influence neural development. Annu Rev Neurosci 12:491–516
Schmidt ML, Lee VM-Y, Trojanowski JQ (1991) Comparative epitope analysis of neuronal cytoskeletal proteins in Alzheimer disease senile plaque neurites and neuropil threads. Lab Invest 64:352–357
Shoji M, Hirai S, Yamaguchi ZH, Harigaya Y, Kawarabayashi T (1990) Amyloid β-protein precursor accumulates in dystrophic neurites of senile plaques in Alzheimer-type dementia. Brain Res 512:164–168
Sternberger LA, Sternberger NH (1983) Monoclonal antibodies distinguish phosphorylated and nonphosphorylated forms of neurofilaments in situ. Proc Natl Acad Sci USA 80:6126–6130
Tagliavini F, Giaconne G, Frangione B, Bugiani O (1988) Preamyloid deposits in the cerebral cortex of patients with Alzheimer's disease and nondemented individuals. Neurosci Lett 93:191–196
Van Nostrand WE, Wagner SL, Suzuki M, Choi BH, Farrow JS, Geddes JW, Cotman CW, Cunningham DD (1989) Protease nexin-11, a potent antichymotripsin, shows identity to amyloid β-protein precursor. Nature 341:546–549
Whitehouse PJ, Price DL, Clark AW, Coyle JT, Delong MR (1981) Alzheimer disease: evidence for selective loss of cholinergic neurons in the nucleus basalis. Ann Neurol 10: 122–126
Wisniewski HM, Terry RD (1973) Re-examination of the pathogenesis of the senile plaque. Prog Neuropathol 2:1–26
Wolozin BL, Pruchnicki A, Dickson DW, Davies P (1986) A neuronal antigen in the brain of Alzheimer patients. Science 232:648–650
Yamaguchi H, Hirai S, Morimatsu M, Sjoji M, Ihara Y (1988) A variety of cerebral amyloid deposits in the brain of the Alzheimer type dementie demonstrated by β-protein immunostaining. Acta Neuropathol 76:541–549
Yamaguchi H, Nakazato Y, Shoji M, Ihara Y, Hirai S (1990) Ultrastructure of the neuropil threads in the Alzheimer brain: their dendritic origin and accumulation in the senile plaques. Acta Neuropathol 80:368–374
Zhan SS, Beyreuther K, Schmitt HP (1993) Quantitative assessment of the synaptophysin immunoreactivity of the cortical neuropil in various neurodegenerative disorders with dementia. Dementia 4:66–74
Zheng JQ, Felder M, Connor JA, Poo M-m (1994) Turning of nerve growth cones induced by neurotransmitters. Nature 368: 140–144
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Zhan, SS., Kamphorst, W., Van Nostrand, W.E. et al. Distribution of neuronal growth-promoting factors and cytoskeletal proteins in altered neurites in Alzheimer's disease and non-demented elderly. Acta Neuropathol 89, 356–362 (1995). https://doi.org/10.1007/BF00309629
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DOI: https://doi.org/10.1007/BF00309629