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Ultrastructural localization of Alzheimer amyloid β/A4 protein precursor in the cytoplasm of neurons and senile plaque-associated astrocytes

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Summary

The ultrastructural localization of amyloid β/A4 protein precursor (APP) in the brains of control and Alzheimer's disease patients was examined immunohistochemically using antisera against the N and C termini of APP. In both control and Alzheimer brains, immunoreaction for APP was seen in the cytoplasm of most neurons, on plasma membranes, outer membrane of mitochondria, granular substance and neurofilaments. Cell bodies and foot processes of astrocytes, containing glial filaments, were also labeled. In primitive and classic type senile plaques, APP immunoreaction products were localized in the astroglial processes that surrounded the amyloid mass of the senile plaques. Swollen degenerating neurites in the senile plaques were also labeled. Amyloid fibrils were negative with APP antisera.

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Authors and Affiliations

  1. College of Medical Care and Technology, School of Medicine, Gunma University, 3-39-15 Showa-machi, 371, Maebaschi, Gunma, Japan

    Haruyasu Yamaguchi

  2. Department of Neurology, School of Medicine, Gunma University, 3-39-15 Showa-machi, 371, Maebaschi, Gunma, Japan

    Haruyasu Yamaguchi, Tsuneo Yamazaki, Koji Ishiguro, Mikio Shoji & Shunsaku Hirai

  3. Department of Pathology, School of Medicine, Gunma University, 3-39-15 Showa-machi, 371, Maebaschi, Gunma, Japan

    Yoichi Nakazato

Authors
  1. Haruyasu Yamaguchi
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  4. Mikio Shoji
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Supported by Grant-in-Aid for Scientific Research on Priority Area 02240105 from the Ministry of Education, Science, and Culture, Japan

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Yamaguchi, H., Yamazaki, T., Ishiguro, K. et al. Ultrastructural localization of Alzheimer amyloid β/A4 protein precursor in the cytoplasm of neurons and senile plaque-associated astrocytes. Acta Neuropathol 85, 15–22 (1992). https://doi.org/10.1007/BF00304629

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  • DOI: https://doi.org/10.1007/BF00304629

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