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Immunohistochemical localization of keratin in bull, goat, and sheep anterior pituitary glands

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Summary

Immunohistochemical localization of keratin, an intermediate filament protein, was studied in bull, goat, and sheep anterior pituitary glands, i.e., in animals of the order Artiodactyla. Strong immunoreactivity was detected in the cells of the marginal layer of bull and goat, as well as in cysts or large follicles in the anterior lobe of all 3 species. In addition, a number of stellateshape cells were immunoreactive for keratin and were distributed throughout the anterior lobe. The localization of keratin-positive cells in light-microscopic preparations correlated precisely with the localization of folliculo-stellate cells in adjacent ultrathin sections. In ultrastructural studies, many slender and elliptical membranous components which were different from smooth endoplasmic reticulum were observed in the cytoplasm of the some keratin-positive cells. Some of the folliculostellate cells in the 3 species were also immunoreactive for the α subunit of S-100 protein, which exists in some epithelial cells. On the other hand, immunolocalization of glial fibrillary acidic protein, a glial cell marker, could not be demonstrated in the anterior pituitary glands of the 3 species studied. These results suggest that keratin-positive folliculo-stellate cells express epithelial-like characteristics.

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References

  • Asa SL, Kovacs K, Bilbao JM, Penz G (1981) Immunohistochemical localization of keratin in craniopharyngiomas and squamous cell nests of the human pituitary. Acta Neuropathol (Berl) 54:257–260

    Google Scholar 

  • Asa SL, Kovacs K, Bilbao JM (1983) The pars tuberalis of the human pituitary: a histologic, immunohistochemical, ultrastructural and immunoelectron microscopic analysis. Virchows Arch [A] 399:49–59

    Google Scholar 

  • Beauvillain JC, Tramu G, Dubois MP (1975) Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in lerot pituitary (Eliomys quercinus): a superimposition technique and an immunocytochemical technique. Cell Tissue Res 158:301–317

    Google Scholar 

  • Cocchia D, Miani N (1980) Immunocytochemical localization of the brain-specific S-100 protein in the pituitary gland of adult rat. J Neurocytol 9:771–782

    Google Scholar 

  • Dingemans KP, Feltkamp CA (1972) Nongranulated cells in the mouse adenohypophysis. Z Zellforsch 124:387–405

    Google Scholar 

  • Farquhar MG (1957) “Corticotrophs” of the rat adenohypophysis as revealed by electron microscopy. Anat Rec 127:291

    Google Scholar 

  • Forte TM, Machen TE, Forte GJ (1977) Ultrastructural changes in oxyntic cells associated with secretory function: a membranerecycling hypothesis. Gastroenterology 73:941–955

    Google Scholar 

  • Fukuda T (1973) Agranular stellate cells (so-called follicular cells) in human fetal and adult adenohypophysis and in pituitary adenomas. Virchows Arch [A] 359:19–30

    Google Scholar 

  • Girod C, Trouillas J, Dubois MP (1985) Immunocytochemical localization of S-100 protein in stellate cells (folliculostellate cells) of the anterior lobe of the normal human pituitary. Cell Tissue Res 241:505–511

    Google Scholar 

  • Haimoto H, Hosoda S, Kato K (1987) Differential distribution of immunoreactive S100-α and S100-β proteins in normal nonnervous human tissues. Lab Invest 57:489–498

    Google Scholar 

  • Höfler H, Denk H, Walter GF (1984a) Immunohistochemical demonstration of cytokeratins in endocrine cells of the human pituitary gland and in pituitary adenomas. Virchows Arch [A] 404:359–368

    Google Scholar 

  • Höfler H, Walter GF, Denk H (1984b) Immunohistochemistry of folliculo-stellate cells in normal human adenohypophyses and in pituitary adenomas. Acta Neuropathol (Berl) 65:35–40

    Google Scholar 

  • Horvath E, Kovacs K, Penz G, Ezrin C (1974) Origin, possible function and fate of “follicular cells” in the anterior lobe of the human pituitary: An electron microscopic study. Am J Pathol 77:199–212

    Google Scholar 

  • Hsu SM, Rain L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    Google Scholar 

  • Ironside JW, Royds JA, Jefferson AA, Timperley WR (1987) Immunolocalisation of cytokeratins in the normal and neoplastic human pituitary gland. J Neurol Neurosurg Psychiatry 50:57–65

    Google Scholar 

  • Isobe T, Ishioka N, Masuda T, Takahashi Y, Ganno S, Okuyama T (1983) A rapid separation of S100 subunits by high performance liquid chromatography: the subunit compositions of S100 proteins. Biochem Int 6:419–426

    Google Scholar 

  • Lauriola L, Cocchia D, Sentinelli S, Maggiano N, Maira G, Michetti F (1984) Immunohistochemical detection of folliculo-stellate cells in human pituitary adenomas. Virchows Arch [B] 47:189–197

    Google Scholar 

  • Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space Nature 283:249–256

    Google Scholar 

  • Millonig G (1962) Further observation on a phosphate buffer for osmium solutions in fixation. In: Electron Microscopy, Fifth International Congress for Electron Microscopy, vol 2. Academic Press, New York, p 8

    Google Scholar 

  • Moll R, Franke WW, Schiller DL, Greiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24

    Google Scholar 

  • Morris C, Hitchcock E (1985) Immunocytochemistry of folliculostellate cells of normal and neoplastic human pituitary gland. J Clin Pathol 38:481–488

    Google Scholar 

  • Nakajima T, Yamaguchi H, Takahashi K (1980) S 100 protein in folliculostellate cells of the rat pituitary anterior lobe. Brain Res 191:523–531

    Google Scholar 

  • Nakazato Y, Yamaguchi H, Takahashi K, Mori T (1982) Immunohistochemical localization of nervous tissue proteins S-100, GFAP and astroprotein in extraneural tissue. In: Peters H (ed) Protides of biological fluids. Pergamon Press, Oxford, pp 47–50

    Google Scholar 

  • Salazar H (1968) Ultrastructural evidence for the existence of a non-secretory, sustentacular cell in the human adenohypophysis. Anat Rec 160:419–420

    Google Scholar 

  • Schlegel R, Banks-Schlegel S, Pinkus GS (1980) Immunohistochemical localization of keratin in normal human tissues. Lab Invest 42:91–96

    Google Scholar 

  • Shirasawa N (1986) Differentiation of pituitary folliculo-stellate cells. In: Yoshimura F, Gorbman A (eds) Pars distalis of the pituitary gland: structure, function and regulation. Elsevier, Amsterdam, pp 175–181

    Google Scholar 

  • Shirasawa N, Yoshimura F (1982) Immunohistochemical and electron microscopical studies of mitotic adenohypophysial cells in different ages of rats. Anat Embryol 165:51–61

    Google Scholar 

  • Shirasawa N, Kihara H, Yamaguchi S, Yoshimura F (1983) Pituitary folliculo-stellate cells immunostained with S-100 protein antiserum in postnatal, castrated and thyroidectomized rat. Cell Tissue Res 231:235–249

    Google Scholar 

  • Shirasawa N, Yamaguchi S, Yoshimura F (1984) Granulated folliculo-stellate cells and growth hormone cells immunostained with anti-S 100 protein serum in the pituitary glands of the goat. Cell Tissue Res 237:7–14

    Google Scholar 

  • Shirasawa N, Mitui J, Mera F, Ishikawa H (1988) Comparative immunohistochemical study of the mammalian pituitary cells stained with antisera against S-100 protein and non-neuronal enolase. Biomed Res 9:477–487

    Google Scholar 

  • Stoeckel ME, Schmitt G, Porte A (1981) Fine structure and cytochemistry of the mammalian pars intermedia. In: Peptides of pars intermedia. Ciba Foundation Symposium 81. Pitman, London, pp 101–127

    Google Scholar 

  • Svalander C (1974) Ultrastructure of the fetal rat adenohypophysis. Acta Endocrinol (Copenh) 76 [Suppl 188]:1–133

    Google Scholar 

  • Takahashi K, Isobe T, Ohtsuki Y, Akagi T, Sonobe H, Okuyama T (1984) Immunohistochemical study on the distribution of α and β subunits of S-100 protein in human neoplasm and normal tissues. Virchow Arch [B] 45:385–396

    Google Scholar 

  • Velasco ME, Roessmann U, Gambetti P (1982) The presence of glial fibrillary acidic protein in the human pituitary gland. J Neuropathol Exp Neurol 41:150–163

    Google Scholar 

  • Yoshida Y (1966) Electron microscopy of the anterior pituitary gland under normal and different experimental conditions. Meth Achievm Exp Pathol 1:439–454

    Google Scholar 

  • Yoshimura F, Nogami H (1981) Fine structural criteria for identifying rat corticotrophs. Cell Tissue Res 219:221–228

    Google Scholar 

  • Yoshimura F, Soji T, Sato S, Yokoyama M (1977) Development and differentiation of rat pituitary follicular cells under normal and some experimental conditions with special references to an interpretation of renewal cell system. Endocrinol Jpn 24:435–449

    Google Scholar 

  • Yoshimura F, Nogami H, Shirasawa N, Yashiro T (1981) A whole range of fine structural criteria for immunohistochemically identified LH cells in rats. Cell Tissue Res 217:1–10

    Google Scholar 

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  1. Department of Anatomy, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, 105, Tokyo, Japan

    Takaki Shimada

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Shimada, T. Immunohistochemical localization of keratin in bull, goat, and sheep anterior pituitary glands. Cell Tissue Res 267, 251–260 (1992). https://doi.org/10.1007/BF00302962

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  • DOI: https://doi.org/10.1007/BF00302962

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