Abstract
Two Giemsa banding methods (C banding and RB banding) are described which selectively stain the centromere bands of polytene salivary gland chromosomes in a number of Chironomus species. — By the C banding method the polytene chromosome appearance is changed grossly. Chromosome bands, as far as they are identifiable, are stained pale with the exception of the centromere bands and in some cases telomeres, which then are intensely stained reddish blue. — By the RB method the centromere bands are stained bright blue, whereas the remainder of the polytene bands stain red to red-violet. — Contrary to all other species examined, in Chironomus th. thummi numerous interstitial polytene chromosome bands, in addition to the centromere regions, are positively C banded and blue stained by RB banding. In the hybrid of Ch. th. thummi x Ch. th. piger only those interstitial thummi bands which are known to have a greater DNA content than their homologous piger bands are C banding positive and blue stained by the RB method whereas the homologous piger bands are C banding negative and red stained by RB banding. Ch. thummi and piger bands with an equal amount of DNA both show no C banding and stain red by RB banding. — It seems that the Giemsa banding methods used are capable of demonstrating, in addition to centromeric heterochromatin, heterochromatin in those interstitial polytene chromosome bands whose DNA content has been increased during chromosome evolution.
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Arrighi, F.E., Hsu, T.C.: Localization of heterochromatin in human chromosomes. Cytogenetics 10, 81–86 (1971)
Arrighi, F.E., Hsu, T.C., Saunders, P., Saunders, G.F.: Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization. Chromosoma (Berl.) 32, 224–236 (1970)
Bedo, D.G.: C banding in polytene chromosomes of Simulium ornatipes and S. melatum (Diptera: Simuliidae). Chromosoma (Berl.) 51, 291–300 (1975)
Burkholder, G.D.: The ultrastructure of G- and C-banded chromosomes. Exp. Cell Res. 90, 269–278 (1975)
Busch, H.: Histones and other nuclear protein. New York-London: Academic Press 1965
Comings, D.E., Avelino, E., Okada, T.A., Wyandt, H.E.: The mechanism of C- and G-banding of chromosomes. Exp. Cell Res. 77, 469–493 (1973)
Hägele, K.: DNS-Replikationsmuster der Speicheldrüsen-Chromosomen von Chironomiden. Chromosoma (Berl.) 31, 91–138 (1970)
Hägele, K.: Prolongation of replication time after doublings of the DNA content of polytene chromosome bands of Chironomus. Chromosoma (Berl.) 55, 253–258 (1976)
Keyl, H.-G.: Chromosomenevolution bei Chironomus. II. Chromosomenumbauten und phylogenetische Beziehungen der Arten. Chromosoma (Berl.) 13, 464–514 (1962)
Keyl, H.-G.: Duplikationen von Untereinheiten der chromosomalen DNS während der Evolution von Chironomus thummi. Chromosoma (Berl.) 17, 139–180 (1965)
Keyl, H.-G., Pelling, C.: Differentielle DNS-Replikation in den Speicheldrüsen-Chromosomen von Chironomus thummi. Chromosoma (Berl.) 14, 347–359 (1963)
Lima-de-Faria, A., Jaworska, H.: Late DNA synthesis in heterochromatin. Nature (Lond.) 217, 138–142 (1968)
Martin, J.: A review of the Genus Chironomus (Diptera, Chironomidae). IX. The cytology of Chironomus tepperi Skuse. Chromosoma (Berl.) 45, 91–98 (1974)
Pathak, S., Arrighi, F.E.: Loss of DNA following C-banding procedures. Cytogenet. Cell Genet. 12, 414–422 (1973)
Scheres, J.M.J.C.: CT banding of human chromosomes. Description of the banding technique and some of its modification. Hum. Genet. 31, 293–307 (1976)
Schmiady, H., Wegner, R.-D., Sperling, K.: Relative DNA content of human euchromatin and heterochromatin after G, C and Giemsa 11 banding. Humangenetik 23, 311–314 (1975)
White, M.J.D.: The chromosomes. London: Chapmann and Hall 1973
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Hägele, K. Differential staining of polytene chromosome bands in Chironomus by Giemsa banding methods. Chromosoma 59, 207–216 (1977). https://doi.org/10.1007/BF00292778
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DOI: https://doi.org/10.1007/BF00292778