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C-Banding of Plant Chromosomes

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Plant Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1429))

Abstract

C-banding is used to differentially stain metaphase chromosomes in organisms having appreciable amounts of constitutive heterochromatin. Its primary benefits are that it is an inexpensive and a relatively fast method of identifying individual chromosomes and morphological or karyotypic variation, including large chromosomal rearrangements and aneuploidies. We currently employ this technique with considerable effect in genome analysis of oat (Avena sativa) and related grass species, though it has been most extensively used for chromosome analysis of wheat (Triticum aestivum) and its relatives of the Triticeae.

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References

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Author information

Authors and Affiliations

  1. Department of Plant and Wildlife Sciences, Brigham Young University, 5009 LSB, Provo, UT, 84602, USA

    Eric N. Jellen

Authors
  1. Eric N. Jellen
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Corresponding author

Correspondence to Eric N. Jellen .

Editor information

Editors and Affiliations

  1. University of Minnesota, St. Paul, Minnesota, USA

    Shahryar F. Kianian

  2. University of Minnesota, St. Paul, Minnesota, USA

    Penny M. Avoles Kianian

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Jellen, E.N. (2016). C-Banding of Plant Chromosomes. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3622-9_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3620-5

  • Online ISBN: 978-1-4939-3622-9

  • eBook Packages: Springer Protocols

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