Skip to main content
SpringerLink
Log in

Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster

  • Original Articles
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The gene that encodes phosphoglycerate kinase in Drosophila melanogaster (Pgk) has been isolated and characterized. There is a single copy of Pgk in the Drosophila genome located at cytogenetic position 23A1-2. Transcripts of Pgk are 1.6 kb long and are found during development with a profile similar to the expression pattern of other genes of the glycolytic pathway. There are substantial amounts of maternal transcript in early embryos which decline in abundance until mid-embryogenesis when transcript levels increase; levels remain high, during larval stages, fall during pupariation and rise again at emergence. The nucleotide sequence of the Pgk gene reveals two small introns, one of which is at a position identical to the site of an intron found in Pgk genes from other organisms. The Pgk gene has no TATA box in the region of transcription initiation and has multiple transcription initiation sites that are closely spaced within 110 nucleotides of the translation start site.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adra CN, Boer PH, McBurney MW (1987) Cloning and expression of the mouse Pgk-1 gene and the nucleotide sequence of its promoter. Gene 60:65–74

    Google Scholar 

  • Banks RD, Blake CCF, Evans PR, Haser R, Rice DW, Hardy GW, Merrett M, Philipps AW (1979) Sequence, structure and activity of phosphoglycerate kinase: a possible hinge-bending enzyme. Nature 279:773–777

    Google Scholar 

  • Bender W, Spierer P, Hogness DS (1975) Chromosomal walking and jumping to isolate DNA from the Ace and rozy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol 168:17–33

    Google Scholar 

  • Blake CCF, Evan PR, Scopes RK (1972) Structure of horse-muscle phosphoglycerate kinase at 6 Å resolution. Nature 235:195

    Google Scholar 

  • Boer PH, Adra CN, Lau F, McBurney MW (1987) The testisspecific phosphoglycerate kinase gene Pgk-2 is a recruited retroposon. Mol Cell Biol 7:3107–3122

    Google Scholar 

  • Bryant TN, Watson HC, Wendell PL (1974) Structure of yeast phosphoglycerate kinase. Nature 247:14–17

    Google Scholar 

  • Calzone FJ, Britten RJ, Davidson EH (1987) Mapping of gene transcripts by nuclease protection and cDNA primer extension. Methods Enzymol 152:611–632

    Google Scholar 

  • Chew GK, Cooper DW (1973) Phosphoglycerate kinase polymorphism in Drosophila. Biochem Genet 8:267–270

    Google Scholar 

  • Ciccarese S, Tommasi S, Vonghia G (1989) Cloning and cDNA sequencing of the rat X-chromosome linked phosphoglycerate kinase. Biochem Biophys Res Commun 165:1337–1344

    Google Scholar 

  • Clements JM, Roberts CF (1986) Transcription and processing signal in the 3-phosphoglycerate kinase (Pgk) gene from Aspergillus nidulans. Gene 44:97–105

    Google Scholar 

  • Eicher EM, Cherry M, Flaherty L (1978) Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex. Mol Gen Genet 158:225–228

    Google Scholar 

  • Fujii H, Yoshida A (1980) Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemia. Proc Nail Acad Sci USA 77:5461–5465

    Google Scholar 

  • Fujii H, Krietsch WKG, Yoshida A (1980) A single amino acid substitute (Asp to Asn) in a phosphoglycerate kinase variant (Pgk Munchen) associated with enzyme deficiency. J Biol Chem 255:6421–6423

    Google Scholar 

  • Fujii H, Chen SH, Akatsuka J, Miwa S, Yoshida A (1981) Use of cultured lymphoblastoid cells for the study of abnormal enzymes: molecular abnormality of a phosphoglycerate kinase variant associated with hemolytic anemia. Proc Natl Acad Sci USA 78:2587–2590

    Google Scholar 

  • Huang IY, Welch CD, Yoshida A (1980) Complete amino acid sequence of human phosphoglycerate kinase. J Biol Chem 255:6412–6420

    Google Scholar 

  • Hultmark D, Klemenz R, Gehring WJ (1986) Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22. Cell 44:429–438

    Google Scholar 

  • von Kalm L, Weaver J, DeMarco J, Maclntyre RJ, Sullivan DT (1989) Structural characterization of the α-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster. Proc Nail Acad Sci USA 86:5020–5024

    Google Scholar 

  • Lissemore JL, Colbert JT, Quail PH (1987) Cloning of cDNA for phytochrome from etiolated Cucurbita and coordinate photoregulation of two distinct phytochrome transcripts. Plant Mol Biol 8:485–496

    Google Scholar 

  • Mas MT, Chen CY, Hitzeman RA, Riggs AD (1986) Active human-yeast chimeric phosphoglycerate kinases engineered by domain interchange. Science 233:788–790

    Google Scholar 

  • McCarrey JR (1987) Nucleotide sequence of the promoter region of a tissue-specific human retroposon: comparison with its housekeeping progenitor. Gene 61:291–298

    Google Scholar 

  • McCarrey JR, Thomas K (1987) Human testis-specific Pgk gene lacks introns and possesses characteristics of a processed gene. Nature 326:501–505

    Google Scholar 

  • Michelson AM, Markham AF, Orkin SH (1983) Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Nat Acad Sci USA 80:472–476

    Google Scholar 

  • Michelson AM, Blake CC, Evans ST, Orkin SH (1985a) Structure of the human phosphoglycerate kinase gene and the intronmediated evolution and dispersal of the nucleotide-binding domain. Proc Natl Acad Sci USA 82:6965–6969

    Google Scholar 

  • Michelson AM, Bruns GA, Morton CC, Orkin SH (1985b) The human phosphoglycerate kinase multigene family. J Biol Chem 260:6982–6992

    Google Scholar 

  • Mori N, Singer-Sam J, Lee C-Y, Riggs AD (1986) The nucleotide sequence of a cDNA clone containing the entire coding sequence for mouse X-chromosome linked phosphoglycerate kinase. Gene 45:275–280

    Google Scholar 

  • Mount SM (1982) A catalog of splice junction sequences. Nucleic Acids Res 10:459–472

    Google Scholar 

  • Nellmann LJ, Holm F, Atlung T, Hansen FG (1989) Cloning and characterization of the E. coli phosphoglycerate kinase gene. Gene 77:185–191

    Google Scholar 

  • Pegoraro B, Lee CY (1978) Purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse. Biochim Biophys Acta 522:423–433

    Google Scholar 

  • Pegoraro B, Ansari AA, Lee CY, Erickson RP (1978) Immunological relatedness of two isozymes of 3-phosphoglycerate kinase from the mouse. FEBS Lett 95:371–374

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Shaw-Lee RL, Lissemore JL, Sullivan DT (1991) Structure and expression of the triose phosphate isomerase (Tpi) gene of Drosophila melanogaster. Mol Gen Genet 230:225–229

    Google Scholar 

  • Shaw-Lee RL, Lissemore JL, Sullivan DT, Tolan DR (1992) Alternative splicing of the fructose 1, 6, bisphosphate aldolase transcript in Drosophila melanogaster predicts three isozymes. J Biol Chem 267:3959–3967

    Google Scholar 

  • Singer-Sam J, Keith DH, Tani K, Simmer RL, Shively L, Lindsay S, Yoshida A, Riggs AD (1984) Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase. Gene 32:409–417

    Google Scholar 

  • Skuse GR, Sullivan DT (1985) Developmentally regulated alternate modes of expression of the Gpdh locus of Drosophila. EMBO J 4:2275–2280

    Google Scholar 

  • van Solingen P, Muurling H, Koekman B, Berg JVD (1988) Sequence of the Penicillium chrysogenum phosphoglycerate kinase gene. Nucleic Acids Res 16:11823

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Szabo P, Grzeschik KH, Siniscalco M (1984) A human autosomal phosphoglycerate kinase locus maps near the HLA cluster. Proc Natl Acad Sci USA 81:3167–3169

    Google Scholar 

  • Vanhanen S, Lehtovaara L, Knowles J (1989) Isolation and characterization of the 3-phosphoglycerate kinase gene (Pgk) from the filamentous fungus Trichoderma reesei. Curr Genet 15:181–186

    Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

  • Voelker RA, Ohnishi S, Langley C (1979) Genetic and cytogenetic studies of four glycolytic enzymes in Drosophila melanogaster: aldolase, triosephosphate isomerase, 3-phosphoglycerate kinase and phosphoglucomutase. Biochim Genet 17:769–783

    Google Scholar 

  • Watson HC, Walker NPC, Shaw PJ, Bryant TN, Wendell PL, Fothergill LA, Perkins RF, Conroy SC, Dobson MJ, Tuite MF, Kingsman AJ (1982) Sequence and structure of yeast phosphoglycerate kinase. EMBO J 1:1635–1640

    Google Scholar 

  • Yoshida A, Watanabe S, Chen SH, Giblett ER, Ralcolm LA (1972) Human phosphoglycerate kinase. J Biol Chem 247:446–449

    Google Scholar 

  • Zinn K, DiMaio D, Maniatis T (1983) Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 34:865–879

    Google Scholar 

Download references

Author information

Author notes

  1. Linda Roselli-Rehfuss

    Present address: Vellum Institute for Advanced Research, Oregon Health Sciences University, 3181 SW. Sam Jackson Park Rd., 97201, Portland, Oregon, USA

Authors and Affiliations

  1. Department of Biology, Syracuse University, 130 College Place, 13244, Syracuse, New York, USA

    Linda Roselli-Rehfuss, Feng Ye, James L. Lissemore & David T. Sullivan

Authors
  1. Linda Roselli-Rehfuss
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James L. Lissemore
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David T. Sullivan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by B.H. Judd

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roselli-Rehfuss, L., Ye, F., Lissemore, J.L. et al. Structure and expression of the phosphoglycerate kinase (Pgk) gene of Drosophila melanogaster . Molec. Gen. Genet. 235, 213–220 (1992). https://doi.org/10.1007/BF00279363

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00279363

Key words

Search

Navigation