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Gene dunce Localization in the Polytene Chromosome of Drosophila melanogaster Long Span Batch of Adjacent Chromosomal Structures

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The molecular and chromosomal localization of the dunce gene was studied. This gene (167.3 kb) consists almost entirely of introns, in which a cluster of seven short tissue-specific genes is located. On the basis of the results of FISH analysis of the gene fragments, we established that the dunce gene is located within nine chromosomal structures (four bands and five interbands), which contradicts the common idea that genes are located in only one structure (band or interband) or at the boundary of these structures. Our results are quite unexpected and original and greatly expand the current understanding of the genetic organization of interphase chromosomes.

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REFERENCES

  1. Zhimulev, I.F., Zykova, T.Yu., Demakova, O.V., Goncharov, F.P., Khoroshko, V.A., Semeshin, V.F., Pokholkova, G.V., Boldyreva, L.V., Demidova, D.S., Babenko, V.N., Demakov, S.A., and Belyaeva, E.S., Genetic organization of interphase chromosome bands and interbands in drosophila melanogaster, PLoS One, 2014, vol. 9, no. 7. e101631.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Belyakin, S.N., Christophides, G.K., Alekseyenko, A.A., Kriventseva, E.V., Belyaeva, E.S., Nanayev, R.A., Makunin, I.V., Kafatos, F.C., and Zhimulev, I.F., Genomic analysis of drosophila chromosome underreplication reveals a link between replication control and transcriptional territories, Proc. Natl. Acad. Sci. U. S. A., 2005, vol. 102, no. 23, pp. 8269–8274.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Qiu, Y.H., Chen, C.N., Malone, T., et al., Characterization of the memory gene dunce of drosophila melanogaster, J. Mol. Biol., 1991, vol. 222, no. 3, pp. 553–565.

    Article  CAS  PubMed  Google Scholar 

  4. Qiu, Y. and Davis, R.L., Genetic dissection of the learning/memory gene dunce of Drosophila melanogaster, Genes Dev., 1993, vol. 7, no. 7B, pp. 1447–1458.

    Article  CAS  PubMed  Google Scholar 

  5. Furia, M., D’Avino, P.P., Crispi, S., et al., Dense cluster of genes is located at the ecdysone-regulated 3C puff of Drosophila melanogaster, J. Mol. Biol., 1993, vol. 231, no. 2, pp. 531–538.

    Article  CAS  PubMed  Google Scholar 

  6. Semeshin, V.F., Artero, R., Peres-Alonso, M., and Shloma, V.V., An electron microscopic in situ hybridization of digoxygenin-dUTP labeled DNA probes with Drosophila melanogaster polytene chromosomes, Tsitologiya, 1998, vol. 40, no. 10, pp 893–894.

    Google Scholar 

  7. Bridges, C.B., Salivary chromosome maps: with a key to the banding of the chromosomes of Drosophila melanogaster, J. Hered., 1935, vol. 26, no. 2, pp. 60–64.

    Article  Google Scholar 

  8. Crick, F., General model for the chromosomes of higher organisms, Nature, 1971, vol. 234, no. 5323, pp. 25–27.

    Article  CAS  PubMed  Google Scholar 

  9. Paul, J., General theory of chromosome structure and gene activation in eukaryotes, Nature, 1972, vol. 238, no. 5365, pp. 444–446.

    Article  CAS  PubMed  Google Scholar 

  10. Zhimulev, I.F., Genetic Organization of Polytene Chromosomes, Academic Press, 1999.

    Book  Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    V. A. Khoroshko, G. V. Pokholkova, T. Yu. Zykova &  I. F. Zhimulev

  2. Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    I. S. Osadchiy

  3. Novosibirsk State University, 630090, Novosibirsk, Russia

    I. F. Zhimulev

Authors
  1. V. A. Khoroshko
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  2. G. V. Pokholkova
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  3. T. Yu. Zykova
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  4. I. S. Osadchiy
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  5. I. F. Zhimulev
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Corresponding author

Correspondence to V. A. Khoroshko.

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Translated by M. Batrukova

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Khoroshko, V.A., Pokholkova, G.V., Zykova, T.Y. et al. Gene dunce Localization in the Polytene Chromosome of Drosophila melanogaster Long Span Batch of Adjacent Chromosomal Structures. Dokl Biochem Biophys 484, 55–58 (2019). https://doi.org/10.1134/S1607672919010137

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  • DOI: https://doi.org/10.1134/S1607672919010137

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