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Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.)

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Abstract

An improved system involving a modification of the bead culture system was developed for culturing pea protoplasts. Using this method, sustained divisions and callus growth could be obtained in all 10 cultivars tested. In the best responding cultivar division frequency could be raised from 17% in liquid culture to 80% in the bead system. Shoot regeneration with a reproducible frequency of about 1% could be obtained from protoplast-derived calli in two of the tested cultivars.

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Abbreviations

ABA:

abscisic acid

BA:

N6-benzyladenine

NAA:

α-naphthylacetic acid

2,4-D:

2,4-dichlorophenoxy-acetic acid

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Authors and Affiliations

  1. Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden

    Johanna Puonti-Kaerlas & Tage Eriksson

Authors
  1. Johanna Puonti-Kaerlas
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  2. Tage Eriksson
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Communicated by H. Lörz

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Puonti-Kaerlas, J., Eriksson, T. Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.). Plant Cell Reports 7, 242–245 (1988). https://doi.org/10.1007/BF00272533

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  • DOI: https://doi.org/10.1007/BF00272533

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