Summary
The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions where lactose carrier protein comprises 1.4% of the cytoplasmic membrane protein.
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Communicated by E. Bautz
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Teather, R.M., Müller-Hill, B., Abrutsch, U. et al. Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector. Molec. Gen. Genet. 159, 239–248 (1978). https://doi.org/10.1007/BF00268260
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DOI: https://doi.org/10.1007/BF00268260