Summary
A total of 26 recombination defective mutants of Bacillus subtilis have been tested for their linkage relationships by the recombination index method. In addition to the previously known recA and recB loci, four further linkage groups (recE, recE, recF, recG) could be distinguished. The recD and recF loci map between ade-16 and cysA14 by PBS1 transduction.
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Armstrong, R. L., Harford, N., Kennett, R. H., St. Pierre, M. L., Sueoka, N.: Experimental methods for Bacillus subtilis. In: Methods in enzymology (H. Tabor and C. W. Tabor, eds.), vol. 17 A, 36–59. London: Academic Press 1970
Chestukhin, A. V., Shemyakin, M. F., Kalinina, N. A., Prozorov, A. D.: Some properties of ATP dependent deoxyribonucleases from normal and rec-mutant strains of Bacillus subtilis. Fed. Eur. Biochem. Soc. Letters 24, 121–125 (1972)
Dubnau, D., Davidoff-Abelson, R., Scher, B., Cirigliano, C.: Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: Phenotypic characterization of radiation-sensitive recombination deficient mutants. J. Bact. 114, 273–286 (1973)
Dubnau, D., Davidoff-Abelson, R., Smith, I.: Transformation and transduction in Bacillus subtilis: Evidence for separate modes of recombinant formation. J. molec. Biol. 45, 155–179 (1969)
Gass, K. B., Hill, T. C., Goulian, M., Strauss, B. S., Cozzarelli, N. R.: Altered deoxyribonucleic acid polymerase activity in a methyl methane sulphonate-sensitive mutant of Bacillus subtilis. J. Bact. 108, 364–374 (1971)
Hadden, C. T.: Transformability of DNA polymerase-deficient mutants of Bacillus subtilis. Biochem. biophys. Res. Commun. 51, 501–506 (1973)
Harford, N., Samojlenko, I., Mergeay, M.: Isolation and characterization of recombination defective mutants of Bacillus subtilis. In: Bacterial transformation (L. Archer, ed.) p. 241–267. London: Academic Press 1973
Hill, T., Prakash, L., Strauss, B.: Mutagen stability of alkylation sensitive mutants of Bacillus subtilis. J. Bact. 110, 47–55 (1972)
Hoch, J. A., Anagnostopoulos, C.: Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J. Bact. 103, 295–301 (1970)
Hoch, J. A., Barat, M., Anagnostopoulos, C.: Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J. Bact. 93, 1925–1937 (1967)
Lacks, S., Hotchkiss, R. D.: A study of the genetic material determining an enzyme activity in Pneumococcus. Biochim. biophys. Acta (Amst.) 39, 508–517 (1960)
Naumov, L. S., Prozorov, A. A., Savchenko, G. V., Kalinina, N. A., Chestukhin, A. V., Shemyakin, M. F.: Location of rec342 mutation with the reduced activity of ATP-dependent DNase on the chromosome map of Bacillus subtilis. Genetika (U.S.S.R.) 9, 171–174 (1973)
Okubo, S., Romig, W. R.: Impaired transformability of a Bacillus subtilis mutant sensitive to mitomycin C and ultraviolet light. J. molec. Biol. 15, 440–454 (1966)
Polsinelli, M., Mazza, G., Canosi, U., Falaschi, A.: Genetical and biochemical characterization of Bacillus subtilis mutants altered in transformation. In: Bacterial transformation (L. Archer, ed.), p. 27–44. London: Academic Press 1973
Prakash, L., Strauss, B.: Repair of alkylation damage: stability of methyl groups in Bacillus subtilis treated with methyl methanesulphonate. J. Bact. 102, 760–766 (1970)
Prozorov, A. A., Azizbekyan, R. R., Barabanshchikov, B. I., Beliayeva, N. N.: Bacillus subtilis mutants with a reduced capability of genetic recombination and a relatively high sensitivity to mitomycin. Genetika (U.S.S.R.) 4, 97–105 (1968)
Searashi, T., Strauss, B.: Relation of the repair of damage induced by a monofunctional alkylation agent to the repair of damage induced by ultraviolet light in Bacillus subtilis. Biochem. biophys. Res. Commun. 20, 680–687 (1965)
Sinha, R. P., Iyer, V. N.: Isolation and some distinctive properties of a new type of recombination deficient mutant of Bacillus subtilis. J. molec. Biol. 72, 711–724 (1964)
Willetts, N. S., Mount, D.: Genetic analysis of recombination-deficient mutants of Escherichia coli K12 carrying rec mutations cotransducible with thyA. J. Bact. 100, 923–934 (1969)
Young, F. E., Wilson, G. A.: Genetics of Bacillus subtilis and other grampositive sporulating bacilli. In: Spores, vol. V (L. L. Campbell, ed.), p. 77–106. Washington, D. C.: American Society for Microbiology 1972
Zadrazil, S., Fucik, V.: Fate of transforming DNA in Bacillus subtilis strain sensitive to methyl methane-sulfonate. Biochem. biophys. Res. Commun. 42, 676–683 (1971)
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Communicated by B. A. Bridges
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Harford, N. Genetic analysis of rec mutants of Bacillus subtilis . Molec. Gen. Genet. 129, 269–274 (1974). https://doi.org/10.1007/BF00267919
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DOI: https://doi.org/10.1007/BF00267919