Summary
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1.
Inhibition of mitochondrial protein synthesis in the petite-negative yeast Kluyveromyces lactis leads to: (i) inhibition of growth in glucose and glycerol, and (ii) loss of antimycin-sensitive respiration and oxidative phosphorylation.
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2.
Loss of antimycin-sensitive respiration results in complete inhibition of growth in glycerol and partial inhibition of growth in glucose.
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3.
The extent to which glucose-growth is inhibited by loss of mitochondrial protein synthesis and/or antimycin-sensitive respiration is found to be strain dependent. The strain differences are explained in terms of different requirements for antimycin-sensitive respiration and oxidative phosphorylation in cell growth and division. However, growth-inhibition by erythromycin cannot be entirely accounted for by respiratory-dependence.
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4.
K. lactis is shown to possess antimycin-insensitive respiration and a cyanide/azide-insensitive respiration. Inhibition of mitochondrial protein synthesis does not lead to loss or change in these alternative respirations, suggesting an extramitochondrial origin for the components of these pathways. The significance of alternative respiration in yeasts is discussed.
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5.
Use of an erythromycin-resistant mutant of K. lactis demonstrates that there is no cross-resistance to chloramphenicol in this yeast strain.
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Communicated by F. Kaudewitz
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Morgan, A.J., Whittaker, P.A. Biosynthesis of yeast mitochondria. Molec. Gen. Genet. 164, 185–193 (1978). https://doi.org/10.1007/BF00267383
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DOI: https://doi.org/10.1007/BF00267383