Summary
A restriction-deficient mutant, Escherichia coli ELA103, was isolated from E. coli AB2463 and used to clone a thr-1 complementing gene from the extremely thermophilic archaebacterium Methanococcus jannaschii. A 7.3 kilobase EcoRI fragment of chromosomal DNA was cloned into pUC8 and the recombinant plasmid designated pELA3471. A Southern blot confirmed M. jannaschii as the source of the inserted fragment, and continued complementation in the presence of a repressor of the the pUC8 lac promoter suggested that the cloned fragment of methanogen DNA included its own transcription signals. The archaebacterium M. jannaschii, which was originally isolated from a deep-sea hydrothermal vent, is the most thermophilic species from which a cloned gene product has been actively expressed in a eubacterium to date.
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Almond, E.L., Clark, A.J. & Clark, D.S. Complementation of a thr-1 mutation of Escherichia coli by DNA from the extremely thermophilic archaebacterium Methanococcus jannaschii . Appl Microbiol Biotechnol 30, 148–152 (1989). https://doi.org/10.1007/BF00264003
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DOI: https://doi.org/10.1007/BF00264003