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Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2522))

Abstract

Genetic manipulation is an essential tool to investigate complex microbiological phenomena. In this chapter we describe the techniques required to transform the model hyperthermophilic, anaerobic archaeon Thermococcus kodakarensis. T. kodakarensis can support two modes of genetic manipulation, dependent either on homologous recombination into the genome or through retention of autonomously replicating plasmids. The robust genetic system developed in T. kodakarensis offers a variety of selectable and counterselectable markers for complex, accurate and iterative genetic manipulations offering greater flexibility to probe gene function in vivo.

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Acknowledgments

We thank members of the Santangelo lab for critical reviews and improvements to the manuscript.

Funding

This work was supported with funding (to TJS) from the National Science Foundation, grant EF-2022065, the US Department of Energy, grant DE-SC0014597, the USA National Institutes of Health, GM100329 and from the USA National Aeronautics and Space Administration, Exobiology Program, 80NSSC20K0613.

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Author notes

  1. Geraldy L. S. Liman and Meghan E. Stettler contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA

    Geraldy L. S. Liman, Meghan E. Stettler & Thomas J. Santangelo

Authors
  1. Geraldy L. S. Liman
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  2. Meghan E. Stettler
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  3. Thomas J. Santangelo
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Corresponding author

Correspondence to Thomas J. Santangelo .

Editor information

Editors and Affiliations

  1. Cellular Biochemistry of Microorganisms - Biochemie III, Universität Regensburg, Regensburg, Bayern, Germany

    Sébastien Ferreira-Cerca

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Liman, G.L.S., Stettler, M.E., Santangelo, T.J. (2022). Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2445-6_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2444-9

  • Online ISBN: 978-1-0716-2445-6

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