Summary
Starch and polyacrylamide gel electrophoresis of seven isozyme systems was investigated as a means of identifying wild and cultivated species of Avena with different ploidy levels. By examining the characteristic isoenzymatic patterns, it was shown that there was considerable variability within the different species. However, it was nevertheless possible to unequivocally identify the species of wild oats and to distinguish between the different species belonging to the same genomic set, thus providing a definitive reference technique for the identification of Avena species in seed-testing laboratories. The relationships between Avena species were inferred from the electrophoresis data. The divergence of the A. maroccana — A. murphyi complex and its contribution to the AACC genomes are emphasized.
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References
Baum BR (1977) Oats: wild and cultivated. Thorn Press, Ottawa
Baum BR, Rajhathy T (1976) A study of Avena macrostachya. Can J Bot 54:2434–2439
Baum BR, Rajhathy T, Sampson DR (1973) An important new diploid Avena species discovered on the Canary Islands. Can J Genet Cytol 51:759–762
Brewer GJ, Sing CF (1970) An introduction to isozymes techniques. Academic Press, New York
Carlson PS (1972) Locating genetic loci with aneuploids. Mol Gen Genet 115:273–280
Cooke RJ, Draper SR (1986) The identification of wild oat species by electrophoresis. Seed Sci Technol 7:517–521
Craig IL, Murray BE, Rajhathy T (1972) Leaf esterase isozymes in Avena and their relationship to the genomes. Can J Genet Cytol 14:581–589
Craig IL, Murray BE, Rajhathy T (1974) A. canariensis: morphological and electrophoretic polymorphism and relationship to the A. magna — A. murphyi complex and A. sterilis. Can J Genet Cytol 16:677–689
Ferguson A (1980) Biochemical systematics and evolution. Blackie, Glasgow
Fernández JA, Jouve N (1987) Chromosomal location of structural genes controlling isozymes in Hordeum chilense. 3. Esterases, glutamate oxalacetate transaminase and phosphoglucomutase. Theor Appl Genet 73:690–698
Fominaya A, Vega C, Ferrer E (1988a) Giemsa C-banded karyotypes of Avena species. Genome 30:627–632
Fominaya A, Vega C, Ferrer E (1988b) C-banding and nucleolar activity of tetraploid Avena species. Genome 30:633–638
Gottlieb LD (1977) Electrophoretic evidence and plant systematics. Ann/Mo Bot Gard 64:161–180
Hartigan J (1983) Cluster analysis of variables. In: Dixon WJ (ed) BMDP statistical software. University of California Press, Berkeley Los Angeles London, pp 447–455
Holden JHW (1966) Species relationships in Avenae. Chromosoma 20:75–124
Jain SK, Singh RS (1979) Population biology of Avena. VII. Allozyme variation in relation to the genome analysis. Bot Gaz Chicago 140:356–362
Kim SI, Mossé J (1979) Electrophoretic patterns of oat prolamines and species relationships in Avena. Can J Genet Cytol 21:309–318
Ladizinsky G (1971) Avena murphyi a new tetraploid species from southern Spain. Isr J Bot 20:24–27
Ladizinsky G, Zohary D (1968) Genetic relationships between diploids and tetraploids in the series Eubarbatae of Avena. Can J Genet Cytol 10:68–81
Ladizinsky G, Zohary D (1971) Note on species delimitation, species relationships and polyploidy in Avena L. Euphytica 20:380–395
Leggett JM (1980) Chromosome relationships and morphological comparisons between the diploid oats Avena prostrata, A. canariensis and the tetraploid A. maroccana. Can J Genet Cytol 22:287–294
Lewontin RC (1974) The genetics basis of evolutionary change. Columbia University Press, New York London
Lookhart GL (1985) Identification of oat cultivars by combining polyacrylamide gel electrophoresis and reversed-phase high-performance liquid chromatography. Cereal Chem 62:345–350
Moore GA, Collins GB (1983) New challenges confronting plant breeders. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding. Elsevier, Amsterdam. Part A, pp 25–58
Murai K, Tsunewaki K (1986) Phylogenetic relationships between Avena species revealed by the restriction endonuclease analysis of chloroplast and mitochondrial DNAs. In: Lames, DA, Thomas H (eds). Proc 2nd Int Oats Conf. The University College of Wales, Welsh Plant Breeding Station, Aberystwyth, 1985, Nijhoff, Amsterdam, pp 34–38
Murray BE, Craig SL, Rajhathy T (1970) A protein electrophoretic study of three amphiploids and eight species in Avena. Can J Genet Cytol 12:651–665
Nielsen G (1984) The use of isozymes as probes to identify and label plant varieties and cultivars. In: Rarrazi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research. Alan R. Liss, New York, pp 1–32
Peterson DM, Brinegar ChA (1986) Oat storage proteins. In: Webster FH (ed) Oats: chemistry and technology. St. Paul pp 153–203
Rajhathy T (1971a) The alloploid model in Avena. Stadler Genet Symp 3:71–87
Rajhathy T (1971b) Chromosome polymorphism in Avena ventricosa. Chromosoma 35:206–216
Rajhathy T, Thomas H (1974) Cytogenetics of oats (Avena L.) Misc Publ Genet Soc Can 2:1–90
Rajhathy T, Shearer DA, Warner EM (1971) A thin layer chromatographic study of some amphiploids in Avena. Can J Genet Cytol 13:749–759
Rao IN, Rao HVP (1980) Evidence for duplicate genes coding for 6-phosphogluconate dehydrogenase in rye. Genet Res 35:309–312
Sadasivaiah RS, Rajhathy T (1968) Genome relationships in tetraploid Avena. Can J Genet Cytol 10:655–669
Salinas J, Benito C (1985) Chromosomal locations of phosphoglucomutase, phosphoglucose isomerase and glutamate oxaloacetate transaminase structural genes in different rye cultivars. Can J Genet Cytol 27:105–113
Sanz J, Fernández JA, Jouve N (1987) Isozymes in Hordeum chilense Brong. var. muticum (Presl.) Hauman. I. Isozyme variation. Cer Res Comm 15:43–50
Shaw CR, Koen AL (1968) Starch gel electrophoresis of isozymes. In: Smith I (ed) Chromatographic and electrophoretic techniques. Interscience, New York, pp 325–364
Singh RS, Jain SK, Qualset CO (1973) Protein electrophoresis as an aid to oat variety identification. Euphytica 22:98–105
Steer MW, Holden JHW, Gunning BES (1970) Avena chloroplast: species relationships and the occurrence of stromacentres. Can J Genet Cytol 12:21–27
Symeonidis L, Tsekos I (1984) Electrophoretic variation in esterase, peroxidases and acid phosphatases in some nature Greek taxa of the genera Hordeum and Taeniatherum. Ann Bot 53:383–397
Tanksley SD, Jones RA (1981) Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort Science 16:179–181
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Communicated by J. MacKey
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de la Hoz, P.S., Fominaya, A. Studies of isozymes in oat species. Theoret. Appl. Genetics 77, 735–741 (1989). https://doi.org/10.1007/BF00261252
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DOI: https://doi.org/10.1007/BF00261252