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The mechanism of uptake of multiple sugars by Saccharomyces cerevisiae in batch culture under fully aerobic conditions

  • Applied Microbiology
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Summary

Kinetic studies of the aerobic batch growth of S. cerevisiae on a glucose substrate, a fructose substrate and a substrate consisting of an equimolar mixture of the two sugars are discussed with reference to possible transport mechanisms. Two systems of uptake were found to be present; one resulting in equal uptake of both glucose and fructose, the other in preferential uptake of glucose to fructose.

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References

  • Barford JP, Hall RJ (1979a) Investigation of the significance of a carbon and redox balance to the measurement of gaseous metabolism of Saccharomyces cerevisiae. Biotechnol Bioeng 21:609–626

    Article  CAS  Google Scholar 

  • Barford JP, Hall RJ (1979b) An examination of the crabtree effect in Saccharomyces cerevisiae: The role of respiratory adaptation. J Gen Microbiol 114:267–275

    Article  CAS  Google Scholar 

  • Barnett JA (1976) The utilization of sugars by yeasts. Adv Carbohydr Res 6:126–228

    Google Scholar 

  • Brooks JD, MacLennon DG, Barford JP, Hall RJ (1981) Design of laboratory continuous culture equipment to accurate kinetic and mass balance experiments. Biotechnol Bioeng 24:847–886

    Article  Google Scholar 

  • Burger M, Hejmova L, Kleinzeller A (1959) Transport of some mono-and di-saccharides into yeast cells. Biochem J 71:233–242

    Article  CAS  Google Scholar 

  • Cirillo VP (1961) The mechanism of sugar transport into the yeast cell. NY Academy Sci Trans, Series B23:725–734

    Article  Google Scholar 

  • Cirillo VP (1961) Mechanism of sugar transport into the yeast cell membrane. J Bacteriol 84:485–491

    Google Scholar 

  • Deak T (1978) On the existence of H+ symport in yeasts, a comparative study. Arch Microbiol 116:205–211

    Article  CAS  Google Scholar 

  • Franzusoff A, Cirillo VP (1982) Uptake and phosphorylation of 2-deoxy-d-glucose by wild-type and single kinase strains of Saccharomyces cerevisiae. Biochim Biophys Acta 688:295–304

    Article  CAS  Google Scholar 

  • Gottschalk A (1947) Further observations on the non-fermentability of d-fructopyranose by yeast. Biochem J 41:478–480

    Article  CAS  Google Scholar 

  • Isbell HS, Pigman WW (1938) Pyranose-furanose. Interconversions with reference to the mutorations of galactose, levulose, lactulose and turanose. Nat Stand Bur Res J 20:773–798

    Article  CAS  Google Scholar 

  • Jaspers HTA, Steveninck van J (1975) Transport associated phosphorylation of 2-deoxy-d-glucose in Saccharomyces fragilis. Biochim Biophys Acta 406:370–385

    Article  CAS  Google Scholar 

  • Jeffery PM (1981) Kinetics of respiration in yeast growth. Ph. D. thesis. University of New South Wales, Australia

  • Kotyk A (1967) Properties of the sugar carrier in baker's yeast. II Specificity of Transport. Folia Microbiol 12:121–131

    Article  CAS  Google Scholar 

  • Meredith SA, Romano AH (1977) Uptake and phosphorylation of 2-deoxy-d-glucose by wild type and respiration deficient baker's yeast. Biochim Biophys Acta 497:745–759

    Article  CAS  Google Scholar 

  • Orlowski JH, Barford JP (1984) The effect of inoculum preparation on the growth of Saccharomyces cerevisiae. 6th Aust Biotechnol Conference, Brisbane, 303–311

  • Panchal CJ, Stewart GG (1979) Utilisation of wort carbohydrates. The Brewers Digest, June, 36–46

  • Panchal CJ, Stewart GG (1982) The influence of media conditions on the utilization of monosaccharides by a strain of Saccharomyces uvarum (carlsbergensis). Inst Brew J 88:86–89

    Article  CAS  Google Scholar 

  • Peynaud E, Domercq S (1955) Sur les especes de levures fermentant selectivement le fructose. Ann Inst Pasteur 89:347–351

    Google Scholar 

  • Prince IG (1983) Tower fermentation for power ethanol production. Ph. D. thesis, Sydney University, Australia

  • Romano AH (1982) Facilitated diffusion of 6-deoxy-d-glucose in baker's yeast: Evidence against phosphorylation associated transport of glucose. J Bacteriol 152 (3):1295–1297

    CAS  PubMed  PubMed Central  Google Scholar 

  • Slator A (1908) Studies in fermentation. (II) Mechanism of alcoholic fermentation. Chem Soc J 93:217–242

    Article  Google Scholar 

  • Sobotka H, Reiner M (1930) Selective fermentation I. Alcoholic fermentation of glucose, fructose and mannose mixtures. Biochem J 24:926–931

    Article  CAS  Google Scholar 

  • Steveninck van J (1968) Transport associated phosphorylation of 2-deoxy-d-glucose in yeast. Biochim Biophys Acta 163:386–391

    Article  Google Scholar 

  • Suomalainen H, Toivonen T (1948) On the fermentability of fructofuranose. Arch Biochem 18:109–118

    CAS  PubMed  Google Scholar 

  • Wilkins PO, Cirillo VP (1965) Sorbose counter flow as a measure of intracellular glucose in baker's yeast. J Bacteriol 90:1605–1610

    CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Sydney University, 2006, NSW, Australia

    Joanna H. Orlowski & John P. Barford

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  1. Joanna H. Orlowski
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  2. John P. Barford
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Orlowski, J.H., Barford, J.P. The mechanism of uptake of multiple sugars by Saccharomyces cerevisiae in batch culture under fully aerobic conditions. Appl Microbiol Biotechnol 25, 459–463 (1987). https://doi.org/10.1007/BF00253319

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  • DOI: https://doi.org/10.1007/BF00253319

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