Summary
The reaction conditions for the enzymatic production of l-cystathionine were optimized, using the two kinds of cystathionine γ-synthase, types I and II, which are abundant in cell-free extracts of Erwinia carotovora (IFO 3830) and Bacillus sphaericus (IFO 3526), respectively. Under the optimal conditions, 178 and 184 mM l-cystathionine (40 and 41 g per liter of the reaction mixture) were synthesized with conversion ratios of 89 and 92% with the Erwinia and Bacillus enzymes, respectively.
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Recipient of a JSPS Fellowship for Japanese Junior Scientists
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Kanzaki, H., Kobayashi, M., Nagasawa, T. et al. Production of l-cystathionine using bacterial cystathionine γ-sythase. Appl Microbiol Biotechnol 25, 322–326 (1987). https://doi.org/10.1007/BF00252541
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DOI: https://doi.org/10.1007/BF00252541