Abstract
Relevant production of xylitol by Debaryomyces hansenii requires semiaerobic conditions since in aerobic conditions the accumulated reduced adenine dinucleotide coenzyme is fully reoxidized leading to the conversion of xylitol into xylulose. For oxygen transfer coefficient values from 0.24 to 1.88 min-1, in shake flasks experiments, biomass formation increased proportionally to the aeration rate as shown in the oxygen transfer coefficient and xylose concentration isoresponse contours. The metabolic products under study, xylitol and ethanol were mainly growth associated. However, for oxygen transfer coefficient above 0.5 min-1 higher initial xylose concentration stimulated the rate of production of xylitol. This fact was less evident for ethanol production. The direct relationship between increased biomass and products formation rate, indicated that the experimental domain in respect to the aeration rate was below the threshold level before the decreasing in metabolic production rates reported in literature for xylose-fermenting yeasts. The fact that ethanol was produced, albeit in low levels, throughout the experimental design indicated that the semiaerobic conditions were always attained. Debaryomyces hansenii showed to be an important xylitol producer exhibiting a xylitol/ethanol ratio above four and a carbon conversion of 54% for xylitol.
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Abbreviations
- KLa:
-
oxygen transfer coefficient
- DO(T):
-
dissolved oxygen (tension)
- OUR:
-
oxygen uptake rate
- NAD(H):
-
oxidised (reduced) nicotinamide adenine dinucleotide
- NADP(H):
-
oxidised (reduced) nicotinamide adenine dinucleotide phosphate
- CRC:
-
catabolic reduction charge
- C:
-
oxygen concentration in the culture medium
- C* :
-
oxygen concentration at saturation conditions
- Yi :
-
response from experiment i
- β:
-
parameters of the polynomial model
- x:
-
experimental factor level (coded units)
- R2 :
-
coefficient of multiple determination
- t:
-
time
References
Alexander MA, Jeffries TW (1990) Respiratory efficiency and metabolite partitioning as regulatory phenomena in yeasts. Enzyme Microb Technol 12:2–19
Amaral-Collaço MT, Gírio FM, Peito MA (1989) Utilization of the hemicellulosic fraction of agro-industrial residues by yeasts. In: Coughlan MP (ed) Enzyme systems for lignocellulosic degradation. Elsevier, London New York, pp 221–230
Baillargeon MW, Jansen NB, Gong C-S, Tsao GT (1983) Effect of oxygen uptake rate on ethanol production by a xylose-fermenting yeast mutant, Candida sp. XF 217. biotechnol Lett 5:339–344
Barbosa MFS, Medeiros MB de, Mancilha IM de, Schneider H, Lee H (1988) Screening of yeasts for production of xylitol from d-xylose and some factors which affect xylitol yield in Candida guilliermondii. J Ind Microbiol 3:241–251
Ditzelmuller G, Kubicek CP, Wohrer W, Rohr M (1984) Xylitol dehydrogenase from Pachysolen tannophilus. FEMS Microbiol Lett 25:195–198
Doehlert DH (1970) Uniform shell designs. Appl Statistics 19:231–239
Du Preez JC, Walt JP van der (1983) Fermentation of d-xylose to ethanol by a strain of Candida shehatae. Biotechnol Lett 5:357–362
Du Preez JC, Prior BA, Monteiro AMT (1984) The effect of aeration on xylose fermentation by Candida shehatae and Pachysolen tannophilus. Appl Microbiol Biotechnol 19:261–266
Du Preez JC, Driessel B van, Prior BA (1989) d-Xylose fermentation by Candida shehatae and Pichia stipitis at low dissolved oxygen levels in fed-batch cultures. Biotechnol Lett 11:131–136
Gírio FM, Peito MA, Amaral-Collaço MT (1989) Enzymatic and physiological study of d-xylose metabolism by Candida shehatae. Appl Microbiol Biotechnol 32:199–204
Grootjen DRJ, Lans RGJM van der, Luyben KChAM (1990) Effects of the aeration rate on the fermentation of glucose and xylose by Pichia stipitis CBS 5773. Enzyme Microb Technol 12:20–23
Jeffries TW (1985) Effects of culture conditions on the fermentation of xylose to ethanol by Candida shehatae. Biotechnol Bioeng Symp 15:149–166
Lee H, Schneider H (1987) Ethanol production by Pichia angophorae. Biotechnol Lett 9:581–584
Ligthelm ME, Prior BA, Du Preez JC (1988) The oxygen requirements of yeasts for the fermentation of d-xylose and d-glucose to ethanol. Appl Microbiol Biotechnol 28:63–68
Lohmeier-Vogel E, Hahn-Hägerdal B (1985) The utilization of metabolic inhibitors for shifting product formation from xylitol to ethanol in pentose fermentations using Candida tropicalis. Appl Microbiol Biotechnol 21:167–172
Meyrial V, Delgenes JP, Moletta R, Navarro JM (1991) Xylitol production from d-xylose by Candida guilliermondii: fermentation behaviour. Biotechnol Lett 13:281–286
Prior BA, Kilian SG, Du Preez JC (1989) Fermentation of d-xylose by the yeasts Candida shehatae and Pichia stipitis. Prospects and problems. Process Biochem 24:21–32
Scheffers WA (1987) Alcohols fermentation. In: The expanding realm of yeast-like fungi. In: Hoog GS de, Smith MT, Wrijman (eds) ACM Proceedings International Symposium on the Perspectives of Taxonomy, Ecology and Phylogeny of Yeasts and Yeast-like Fungi. Elsevier, Amsterdam, pp 321–332
Slininger PJ, Bolen PL, Kurtzman CP (1987) Pachysolen tannophilus: properties and process considerations for ethanol production from d-xylose. Enzyme Microb Technol 9:5–15
Watson NE, Prior BA, Du Preez JC, Lategan PM (1984) Oxygen requirements for d-xylose fermentation to ethanol and polyols by Pachysolen tannophilus. Enzyme Microb Technol 6:447–450
Wise WS (1951) The measurement of the aeration of culture media. J Gen Microbiol 5:167–177
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Roseiro, J.C., Peito, M.A., Gírio, F.M. et al. The effects of the oxygen transfer coefficient and substrate concentration on the xylose fermentation by Debaryomyces hansenii . Arch. Microbiol. 156, 484–490 (1991). https://doi.org/10.1007/BF00245396
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DOI: https://doi.org/10.1007/BF00245396