Summary
Extracellular spikes were recorded simultaneously from dendrites and somata of Purkinje cells in thin cerebellar sections. Spontaneously occurring dendritic spikes were biphasic with the initial phase positive. Triphasic dendritic spikes with a large negative phase appeared during electrophoretic application of glutamate. In media containing procaine, tetrodotoxin, or high concentrations of KCl, negative dendritic spikes occurred whereas soma spikes were abolished. The negative dendritic spikes were suppressed by CoCl2 or MnCl2. Electrical stimulation elicited climbing fibre responses in somata and large negative waves in dendrites. Spikes of dendritic origin were different from those reflecting electrotonic spread of soma spikes. The relation between soma spikes and active dendritic spikes is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blankenship, J.E.: Action of tetrodotoxin on spinal motoneurons of the cat. J. Neurophysiol. 31, 186–194 (1968)
Calvin, W.H., Hellerstein, D.: Dendritic spikes vs. cable properties. Science 163, 96–97 (1969)
Curtis, D.R., Phillis, J.W.: The action of procaine and atropine on spinal neurones. J. Physiol. (Lond.) 153, 17–34 (1960)
Eccles, J.C., Llinás, R., Sasaki, K.: The excitatory synaptic action of climbing fibers on the Purkinje cells of the cerebellum. J. Physiol. (Lond.) 182, 268–296 (1966a)
Eccles, J.C., Llinás, R., Sasaki, K.: The action of antidromic impulses on the cerebellar Purkinje cells. J. Physiol. (Lond.) 182, 316–345 (1966b)
Fujita, Y.: Activity of dendrites of single Purkinje cells and its relation-ship to so-called inactivation response in rabbit cerebellum. J. Neurophysiol. 31, 131–141 (1968)
Furshpan, E.J., Furukawa, T.: Intracellular and extracellular responses of the several regions of the Mauthner cell of the goldfish. J. Neurophysiol. 25, 732–771 (1962)
Haga, T., Abe, T., Kurokawa, M.: Polymerization and depolymerization of microtubules in vitro as studied by flow birefringence. FEES Lett. 39, 291–295 (1974)
Hild, W., Tasaki, I.: Morphological and physiological properties of neurons and glial cells in tissue culture. J. Neurophysiol. 25, 277–304 (1962)
Hubbard, J.I., Llinás, R., Quastel, D.M.J.: Electrophysiological analysis of synaptic transmission. London: Arnold 1969
Kakiuchi, S., Yamazaki, R.: Calcium dependent phosphodiesterase activity and its activating factor (PAF) from brain. Biochem. Biophys. Res. Commun. 41, 1104–1110 (1970)
Llinás, R., Nicholson, C.: Electrophysiological properties of dendrites and somata in aligator Purkinje cells. J. Neurophysiol. 34, 532–551 (1971)
Llinás, R., Hess, R.: Tetrodotoxin-resistant dendritic spikes in avian Purkinje cells. Proc. Natl. Acad. Sci. USA 73, 2520–2523 (1976)
Martinez, F.E., Crill, W.E., Kennedy, T.T.: Electrogenesis of cerebellar Purkinje cell response in cats. J. Neurophysiol. 34, 348–356 (1971)
Okamoto, K., Quastel, J.H.: Spontaneous action potentials in isolated guinea pig cerebellar slices: Effect of amino acids and conditions affecting sodium and water uptake. Proc. R. Soc. Lond. [Biol.] 184, 83–90 (1973)
Rall, W., Burke, R.E., Smith, T.G., Nelson, P.G., Frank, K.: Dendritic location of synapses and possible mechanisms for the monosynaptic EPSP in motoneurons. J. Neurophysiol. 30, 1169–1193 (1967)
Schultz, G., Hardman, J.G., Schultz, K., Baird, C.E., Sutherland, E.W.: The importance of calcium ions for the regulation of guanosine 3',5'-cyclic monophosphate levels. Proc. Natl. Acad. Sci. USA 70, 3889–3893 (1973)
Schwartzkroin, P.A., Slawsky, M.: Probable calcium spikes in hippocampal neurons. Brain Res. 135, 157–161 (1977)
Singh, B.N., Williams, E.M.V.: A fourth class of anti-dysrhythmic action? Effect of verapamil on ouabain toxicity, on atrial and ventricular intracellular potentials, and on other features of cardiac function. Cardiovasc. Res. 6, 109–119 (1972)
Spencer, W.A., Kandel, E.R.: Electrophysiology of hippocampal neurones. IV. Fast prepotentials. J. Neurophysiol. 24, 272–285 (1961)
Taylor, R.E.: Effect of procain on electrical properties of squid axon membrane. Am. J. Physiol. 196, 1071–1078 (1959)
Yamamoto, C.: Electrical activity observed in vitro in thin sections from guinea pig cerebellum. Jpn. J. Physiol. 24, 177–188 (1974)
Yamamoto, C.: Recording of electrical activity from microscopically identified neurons of the mammalian brain. Experientia 31, 309–310 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hounsgaard, J., Yamamoto, C. Dendritic spikes in Purkinje cells of the guinea pig cerebellum studied in vitro. Exp Brain Res 37, 387–398 (1979). https://doi.org/10.1007/BF00237721
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00237721