Summary
A study of the habenular nuclei connections by means of horseradish peroxidase (HRP) has never been carried out in amphibia. In the present paper we have investigated the afferent projections of the left and right habenular nuclei of the frog Rana esculenta using this technique. Cells, labelled by HRP, were either in a Golgi-like pattern or in a granular pattern.
It appears that the habenular nuclei on the two sides of the epithalamus do not show different connections even though they are morphologically asymmetric. In fact, each habenula is connected bilaterally with the septal area and the bed nucleus of the hippocampal commissure, and ipsilaterally with the hypothalamic areas, the entopeduncular nucleus, the periventricular gray of the third ventricle and the interpeduncular nucleus. However, the habenular commissure has typical commissural fibres which apparently do not involve the medial portion of the left habenula.
The habenular connections in the frog are generally similar to those reported in the literature for mammals. In addition, our results show the possibility that HRP is transported both retrograde and anterograde.
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References
Aghajanian G K, Wang R Y (1977) Habenular and other midbrain raphe afferents demonstrated by a modified retrograde tracing technique. Brain Res 122: 229–242
Barry J (1978) Septo-epithalamo-habenular LRF-reactive neurons in monkey. Brain Res 151: 183–187
Beccari N (1943) Neurologia Comparata. Firenze, Sansoni Edizioni Scientifiche
Björklund A (1978) Dopaminergic pathways in the rat CNS. Neuroscience Letters (Suppl) 1: S418
Booth D A (1967) Feeding system in the rat diencephalon. Science 158: 515–517
Boyd E S, Celso M B (1970) Effect of some brain lesions on septal intracranial self-stimulation in the rat. Am J Physiol 219: 734–741
Cajal S R y (1911) Histologie du système nerveux de l'homme et des vertébrés. Maloine, Paris Vol 2, pp 270–275
Cragg B G (1961) The connections of the habenula in the rabbit. Exp Neurol 3: 388–409
Cuello A C, Emson P C, Paxinos G, Jessell J (1978) Substance P containing and cholinergic projections from the habenula. Brain Res 149: 413–429
Emson P C, Cuello A C, Paxinos G, Jessell T, Iversen L L (1977) The origin of substance P and acetylcholine projections to the ventral tegmental area and interpeduncular nucleus in the rat. Acta Physiol Scand [Suppl] 452: 43–46
Filion M, Harnois C (1978) A comparison of projections of entopeduncular neurons to the thalamus, the midbrain and the habenula in the cat. J Comp Neurol 181: 763–780
Fox C A, Schmitz J T (1944) The substantia nigra and the entopeduncular nucleus in the cat. J Comp Neurol 80: 323–334
Frontera J G (1952) A study of the anuran diencephalon. J Comp Neurol 96: 1–69
Gottesfeld Z, Jacobowitz D M (1978) Cholinergic projection of the diagonal band to the interpeduncular nucleus of the rat brain. Brain Res 156: 329–332
Graham R C, Karnovsky M J (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291–302
Guglielmotti V (1979) A thermally controlled microinector. Stain Technol 54: 151–157
Herkenham M, Nauta W J H (1977) Afferent connections of the habenular nuclei in the rat. A horseradish peroxidase study with a note on the fiber-of-passage problem. J Comp Neurol 173: 123–146
Herrick C J (1948) The brain of the tiger salamander. University of Chicago Press, Chicago
Keefer D A (1978) Horseradish peroxidase as a retrogradely-transported detailed dendritic marker. Brain Res 140: 15–32
Kemali M (1977) Morphologic relationship established through the habenulo-interpeduncular system between the right and left portions of the frog brain. In: Structure and function of cerebral commissures. MacMillan Press, London, p 15–33
Kemali M, Braitenberg V (1969) Atlas of the frog brain. Springer, Berlin Heidelberg New York
Kemali M, Guglielmotti V (1977) An electron microscope observation of the right and the two left portions of the habenular nuclei of the frog. J Comp Neurol 176: 133–148
Kim R, Nakano K, Jayaraman A, Carpenter M B (1976) Projections of the globus pallidus and adjacent structures: an autoradiographic study in the monkey. J Comp Neurol 169: 263–290
Kizer J S, Palkovits M, Brownstein M J (1976) The projections of the A8, A9 and A10 dopaminergic cell bodies: evidence for a nigral-hypothalamic-median eminence dopaminergic pathway. Brain Res 108: 363–370
Léranth Cs, Brownstein M, Zaborszky L, Jaranyi Zs, Palkovits M (1975) Morphological and biochemical changes in the rat interpeduncular nucleus following the transection of the habenulo-interpeduncular tract. Brain Res 99: 124–128
Massopust L C Jr, Thompson R (1962) A new interpedunculo-diencephalic pathway in rats and cats. J Comp Neurol 118: 97–105
Mizuno N, Nakamura Y (1974) An electron microscope study of terminal degeneration of the fasciculus retroflexus Meynerti within the interpeduncular nucleus of the rabbit. Brain Res 65: 165–169
Modianos D T, Hitt J C, Flexman J (1974) Habenular lesions produce decrements in feminine, but not masculine sexual behavior in rats. Behav Biol 10: 75–87
Rausch L J, Long C J (1971) Habenular nuclei: a crucial link between the olfactory and motor systems. Brain Res 29: 146–150
Smaha L A, Kaelber W W (1973) Efferent fiber projections of the habenular and the interpeduncular nucleus. An experimental study in the opossum and cat. Exp Brain Res 16: 291–308
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Kemali, M., Guglielmotti, V. & Gioffré, D. Neuroanatomical identification of the frog habenular connections using peroxidase (HRP). Exp Brain Res 38, 341–347 (1980). https://doi.org/10.1007/BF00236654
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DOI: https://doi.org/10.1007/BF00236654