Summary
Immunocytochemical staining has shown that the median neurosecretory cells (MNC) of the brain of the blowfly, Calliphora vomitoria, contain an insulin-like material which cross reacts with antibodies to bovine insulin. There are 24–26 paraldehyde fuchsin-positive MNC of which only 6–8 show the specific insulin-like immunoreactivity.
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Barrington, E.J.W.: Evolutionary aspects of hormonal structure and function. In: Comparative Endocrinology (Gaillard, P.J., Boer, H.H., eds.) 381–396. Amsterdam: Elsevier/North Holland Biomedical Press 1978
Bloch, B., Thomsen, E., Thomsen, M.: The neurosecretory system of the adult Calliphora erythrocephala. III. Electron microscopy of the medial neurosecretory cells of the brain and some adjacent cells. Z. Zellforsch. 70, 185–208 (1966)
Chen, A.C., Friedman, S.: Hormonal regulation of trehalose metabolism in the blowfly, Phormia regina: Interaction between hypertrehalosemic and hypotrehalosemic hormones. J. Insect Physiol. 23, 1223–1232 (1977)
Coons, A.H., Leduc, E.H., Connolly, J.M.: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. Exp. Med. 102, 49–59 (1955)
Duve, H.: The presence of a hypoglucemic and hypotrehalocemic hormone in the neurosecretory system of the blowfly, Calliphora erythrocephala. Gen. Comp. Endocrinol. 36, 102–110 (1978)
Duve, H., Thorpe, A., Lazarus, N.: The isolation of material displaying insulin-like immunological and biological activity from the brain of the blowfly, Calliphora vomitoria. Biochem. J. (In press)
Guillemin, R.: The expanding significance of hypothalamic peptides, or, is endocrinology a branch of neuroendocrinology? Recent Prog. Horm. Res. 33, 1–28 (1977)
Panov, A.A.: The composition of the medial neurosecretory cells of the pars intercerebralis in Calliphora and Lucilia adults. Zool. Anz. Jena 196, 23–27 (1976)
Pearse, A.G.E.: The cytochemistry and ultrastructure of polypeptide hormone producing cells of the APUD series and the embryologic, physiologic and pathologic implication of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)
Pearse, A.G.E., Takor Takor, T.: Neuroendocrine embryology and the APUD concept. Clinical Endocrinology 5, Suppl. 229–244 (1976)
Tager, H.S., Markese, J., Kramer, K.J., Speirs, R.D., Childs, C.N.: Glucagon-like and insulin-like hormones of the insect neurosecretory system. Biochem. J. 156, 515–520 (1976)
Thomsen, M.: I. The neurosecretory system of the adult Calliphora erythrocephala. II. Histology of the neurosecretory cells of the brain and some related structures. Z. Zellforsch. 67, 693–717 (1965)
Thomsen, E., Lea, A.O.: Control of the medial neurosecretory cells by the corpus allatum in Calliphora erythrocephala. Gen. Comp. Endocrinol. 12, 51–57 (1969)
Weyer, F.: Über drüsenartige Nervenzellen im Gehirn der Honigbiene, Apis mellifica L. Zool. Anz. 112, 137–141 (1935)
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Dr. M.C. Thorndyke, Department of Zoology, Bedford College, London University, kindly provided us with fluorescence facilities, and we are also grateful to him for helpful discussions on the immunofluorescence procedure
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Duve, H., Thorpe, A. Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera). Cell Tissue Res. 200, 187–191 (1979). https://doi.org/10.1007/BF00236411
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DOI: https://doi.org/10.1007/BF00236411