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A reliable method for extraction of RNA from various conifer tissues

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Summary

A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.

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Abbreviations

CTAB:

cetyltrimethylammonium bromide

DEPC:

diethylpyrocarbonate

PVP:

polyvinylpyrrolidone

SDS-PAGE:

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada

    Jin-Zhuo Dong & David I. Dunstan

Authors
  1. Jin-Zhuo Dong
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  2. David I. Dunstan
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Communicated by F. Constabel

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Dong, JZ., Dunstan, D.I. A reliable method for extraction of RNA from various conifer tissues. Plant Cell Reports 15, 516–521 (1996). https://doi.org/10.1007/BF00232985

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  • DOI: https://doi.org/10.1007/BF00232985

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