Skip to main content
SpringerLink
Log in

Amyloplast formation in cultured tobacco cells; effects of plant hormones on multiplication, size, and starch content

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

In BY-2 cultured tobacco cells (Nicotiana tabacum L.), depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) and addition of benzyladenine (BA) caused amyloplast formation, a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and facilitated by the addition of BA. An increase in the starch content of BY-2 cells was always accompanied by a reduction in cell multiplication. However, when hormonal conditions were unsuitable for amyloplast formation, the starch content of the cells did not increase, even if cell multiplication was forcibly terminated by the addition of aphidicolin. This result indicates that the hormonal conditions themselves, and not the decrease in cell multiplication, induce amyloplast formation in BY-2 cultured tobacco cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

DAPI:

4′,6-diamidino-2-phenylindole

DMSO:

dimethyl sulfoxide

NAA:

1-naphthylacetic acid

PMSF:

phenyl methyl sulfonyl fluoride

References

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Anal Chem 28: 350–356

    CAS  Google Scholar 

  • Hasezawa S Syono K (1983) Plant Cell Physiol 24: 127–132

    Google Scholar 

  • Ishida S, Takahashi Y, Nagata T (1993) Proc Natl Acad Sci USA 90: 11152–11156

    Google Scholar 

  • Kato K, Matsumoto T, Koiwai A, Mizusaki S, Nishida K, Noguchi M, Tamaki E (1972) In: Terui G (eds) Fermentation Technology today, Society of Fermentation Technologists of Japan, Osaka, pp 689–695

    Google Scholar 

  • Kirk JTO, Tilney-Bassett RAE (1978) The plastids; Their chemistry, structure, growth and inheritance, 2nd edition, Elsevier/North-Holland Biomedical Press, Amsterdam

    Google Scholar 

  • Kuroiwa T (1991) Int Rev Cytol 128: 1–62

    CAS  Google Scholar 

  • Nagata T, Okada K, Takebe I, Matsui C (1981) Mol Gen Genet 184: 161–165

    Google Scholar 

  • Nemoto Y, Kawano S, Nakamura S, Mita T, Nagata T, Kuroiwa T (1988) Plant Cell Physiol 29: 167–177

    CAS  Google Scholar 

  • Sakai A, Kawano S, Kuroiwa T (1992) Plant Physiol 100: 1062–1066

    Google Scholar 

Download references

Author information

Author notes

  1. Kumiko Yashiro

    Present address: Research and Development Division, Kikkoman Co., Ltd., 399 Noda, 278, Chiba, Japan

Authors and Affiliations

  1. Department of Plant Sciences, Graduate School of Science, University of Tokyo, Hongo, 113, Tokyo, Japan

    Atsushi Sakai, Shigeyuki Kawano & Tsuneyoshi Kuroiwa

  2. Department of Botany, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan

    Kumiko Yashiro

Authors
  1. Atsushi Sakai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kumiko Yashiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shigeyuki Kawano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tsuneyoshi Kuroiwa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by A. Komamine

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sakai, A., Yashiro, K., Kawano, S. et al. Amyloplast formation in cultured tobacco cells; effects of plant hormones on multiplication, size, and starch content. Plant Cell Reports 15, 601–605 (1996). https://doi.org/10.1007/BF00232461

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232461

Keywords

Search

Navigation