Summary
Forty single-copy, nuclear probes of known chromosomal position were used to examine restriction fragment length polymorphism in the tomato genus Lycopersion. The probes were from three libraries: one cDNA, and two genomic libraries ⦓ne genomic made with EcoRI and the other with PstI. Total DNA from 156 plants representing eight species was cut with five different restriction enzymes and scored in 198 probe-enzyme combinations. Genetic distances between accessions (populations) and species were calculated from the resultant restriction patterns and proportion of shared bands. Accessions belonging to the same species largely clustered together, confirming their current classification. However, one mountain accession, classified as L. peruvianum var. humifusum (LA2150), was sufficiently distinct from the other accessions of L. peruvianum that it may qualify as a separate species L. esculentum and L. pimpinellifolium were the least clearly differentiated, possibly reflecting introgressive hybridization, known to have been promoted by man in recent history. Dendrograms constructed from cDNA versus genomic clones were nearly identical in their general grouping of species. The dendrograms revealed two major dichotomies in the genus: one corresponding to mating behavior [self-compatible (SC) versus self-incompatible (SI) species] and the other corresponding to fruit color (red versus green-fruited species). The ratio of withinversus between-accession diversity was much lower for SC species, indicating that most of the diversity within these species exists between populations, rather than within populations. Overall, the amount of genetic variation in the SI species far exceeded that found in SC species. This result is exemplified by the fact that more genetic variation could be found within a single accession of one of the SI species (e.g., L. peruvianum) than among all accessions tested of any one of the SC species (e.g., L. esculentum or L. pimpinellifolium). Results from this study are discussed in relationship to germ plasm collection/utilization and with regard to the use of RFLPs in tomato breeding and genetics.
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References
Allard RW (1954) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–278
Bernatzky R, Tanksley SD (1986 a) Genetics of actin-related sequences in tomato. Theor Appl Genet 72:314–321
Bernatzky R, Tanksley SD (1986 b) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898
Bernatzky R, Tanksley SD (1986 c) Methods for detection of single or low copy sequences in tomato on southern blots. Plant Mol Biol Rep 4:37–41
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513
Burr B, Burr RA, Thompson KH, Albertsen MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Ferris SD, Sage RD, Huang C-M, Nielson JT, Ritte U, Wilson AC (1983) Flow of mitochondrial DNA across a species boundary. Proc Natl Acad Sci USA 80:2290–2294
Gottlieb LD (1981) Electrophoretic evidence and plant populations. Phytochemistry 7:1–46
Luckwill LC (1943) The genus Lycopersicon, an historical, biological, and taxonomic survey of the wild and cultivated tomatoes. Aberdeen University Studies, Scotland, No.120
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
McClean PE, Hanson MR (1986) Mitochondrial DNA sequence divergence among Lycopersicon and related Solanum species. Genetics 112:649–667
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Miller JC, Tanksley SD (1990) Effects of restriction enzymes, probe source, and probe length on detecting restriction fragment length polymorphism in tomato. Theor Appl Genet 80:437–448
Muller CH (1940) A revision of the genus Lycopersicon. United States Department of Agriculture, Miscellaneous Publication No. 382
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction frgament length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73:350–356
Palmer JD, Zamir D (1982) Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc Natl. Acad Sci USA 79:5006–5010
Rick CM (1963) Barriers to interbreeding in Lycopersicon peruvianum. Evolution 17:216–232
Rick CM (1976) Tomato. In: Simmonds NW (ed) Evolution of crop plants. Longman, London, pp 262–273
Rick CM (1979) Biosystematic studies in Lycopersicon and closely related species of Solanum. In: Hawkes JC, Lester RN, Skelding AD (eds) The biology of the Solanaceae. Academic Press, New York, pp 1–27
Rick CM (1982) The potential of exotic germ plasm for tomato improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 478–495
Rick CM (1983) Evolution of mating systems: evidence from allozyme variation. In: Genetics: new frontiers. Proc. XV Inter. Congress Genetics, pp 215-221
Rick CM (1986) Reproductive isolation in the Lycopersicon peruvianum complex. In: D'Arcy WG(ed) Solanaceae biology and systematics. Columbia Press, New York, pp 477–495
Roth EJ, Frazier BL, Apuya NR, Lark KG (1989) Genetic variation in an inbred plant: variation in tissue cultures of soybean [Glycine max(L) Merrill]. Genetics 121:359–368
Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759
Schwarz-Sommer Z, Gierl A, Cuypers H, Peterson PA, Saedler orzH (1985) Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J 4:591–597
Sneath PHA, Sokal RR (1973) Numerical taxonomy. WH Freeman, San Francisco
Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a re-examination. Theor Appl Genet 75:811–823
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technol 7:257–264
Warnock SJ (1988) A review of taxonomy and phylogeny of the genus Lycopersicon. HortScience 23:669–673
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the TM-2a gene in tomato. Genetics 120:579–585
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Miller, J.C., Tanksley, S.D. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon . Theoret. Appl. Genetics 80, 437–448 (1990). https://doi.org/10.1007/BF00226743
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DOI: https://doi.org/10.1007/BF00226743