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Phylogenetic relationship and diversity among Agropyron Gaertn. germplasm using SSRs markers

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Abstract

To evaluate the genetic diversity within the genus Agropyron Gaertn. and investigate phylogenetic relationships of species in the genus, 103 populations collected from northern China, including 18 A. desertorum, 12 A. mongolicum, four A. michnoi, and 69 A. cristatum populations, were analyzed using 29 polymorphic simple sequence repeat (SSR) primers mapped on the wheat genome. Mean number of polymorphic alleles in A. cristatum, A. desertorum, A. mongolicum, and A. michnoi was 10.41, 8.66, 6.69 and 4.52, respectively, and were significantly different (P < 0.01). The mean genetic diversity in A. cristatum, A. desertorum, A. mongolicum, and A. michnoi was 0.76, 0.71, 0.63 and 0.62, respectively, and were significantly different (P < 0.05). The largest genetic distance (1.06) among the 103 populations was found between Z1593 and Z1994, two A. cristatum populations, and the smallest genetic distance (0.02) was observed between Z605 and Z618, also two A. cristatum populations. The AMOVA analysis showed 12.0 % of the total variation resided among species, and 88.0 % resided within species. The unweighted pair group method with arithmetic average phenogram indicated the populations from the same species and similar eco-geographical regions had close genetic relationship. Furthermore, populations of A. desertorum, A. mongolicum and A. michnoi were distinguished from populations of A. cristatum using the principal coordinate analysis. The results obtained using SSR markers suggest that A. desertorum, A. mongolicum and A. michnoi should be offspring species of A. cristatum sharing the same basic genome from the counterpart.

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Acknowledgments

The financial support provided by the Department of Science and Technology in Hebei Province, P.R. China (C2009000878) is gratefully appreciated.

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Authors and Affiliations

  1. Department of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, 066600, China

    Yonghe Che & Yanping Yang

  2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, China

    Xinming Yang, Xiuquan Li & Lihui Li

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  1. Yonghe Che
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  2. Yanping Yang
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  3. Xinming Yang
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  4. Xiuquan Li
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Correspondence to Yonghe Che or Lihui Li.

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Che, Y., Yang, Y., Yang, X. et al. Phylogenetic relationship and diversity among Agropyron Gaertn. germplasm using SSRs markers. Plant Syst Evol 301, 163–170 (2015). https://doi.org/10.1007/s00606-014-1062-4

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