Abstract
Sixty-two single-copy sorghum DNA clones were used to compare restriction fragment patterns of 53 sorghum accessions from Africa, Asia and the United States. Included were accessions from five morphological races of the cultivated subspecies bicolor, and four races of the wild subspecies verticilliflorum. From two to twelve alleles were detected with each probe. There was greater nuclear diversity in the wild subspecies (255 alleles in ten accessions) than in the domestic accessions (236 alleles in 37 accessions). Overall, 204 of the 340 alleles (60%) that were detected occurred in both subspecies. Phylogenetic analysis using parsimony separated the subspecies into separate clusters, with one group of intermediate accessions. Though exceptions were common, especially for the race bicolor, accessions classified as the same morphological race tended to group together on the basis of RFLP similarities. Selection for traits such as forage quality may have led to accessions genetically more similar to other races being classified as bicolors, which have a loose, small-grained panicle similar to wild races. Population statistics, calculated using four nuclear and four cytoplasmic probes that detect two alleles each, revealed a low but significant amount of heterozygosity, and showed little differentiation in alleles in the wild and cultivated subspecies. Outcrossing with foreign pollen appears to have been more important than migration via seed dispersal as a mechanism for gene flow between the wild and domestic accessions included in this study.
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Aldrich PR, Doebley J (1992) Restriction fragment variation in the nuclear and chloroplast genomes of cultivated and wild Sorghum bicolor. Theor Appl Genet 85:293–302
Aldrich PR, Doebley J, Schertz KF, Stec A (1992) Patterns of allozyme variation in cultivated and wild Sorghum bicolor. Theor Appl Genet 85:451–460
Berhan AM, Hulbert SH, Bulter LG, Bennetzen JL (1993) Structure and evolution of the genomes of Sorghum bicolor and Zea mays. Theor Appl Genet 86:598–604
Bernatzky R, Tanksley SD (1989) Restriction fragments as molecular markers for germplasm evaluation and utilization. In: Williams JT (ed) The use of plant genetic resources. Cambridge University Press, Cambridge, pp 353–363
Chittenden LM, Schertz KF, Lin YR, Wing RA, Paterson AH (1994) A detailed RFLP map of Sorghum bicolor x S. propinquum suitable for high-density mapping suggests ancestral duplication of Sorghum chromosomes. Theor Appl Genet 87:925–933
Doggett H (1988) Sorghum, 2nd ed. John Wiley and Sons, Inc., New York
Doggett H, Majisu BN (1968) Disruptive selection in crop development. Heredity 23:1–23
Feinberg AP, Vogelstein B (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Bioch 123:6–13
Harlan JR, de Wet JMJ (1972) A simplified classification of cultivated sorghum. Crop Sci 12:172–176
Hartl DL, Clark AG (1989) Principles of population genetics, 2nd ed. Sinauer Associates Inc., Massachusetts, pp 293–323
Helentjaris D, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72:761–769
House LR (1985) A guide to sorghum breeding. 2nd Int Crop Research Institute for the Semi-Arid Tropics, India, pp 3–9
Liang GHL, Casady AJ (1966) Quantitative presentation of the systematic relationship among twenty-one sorghum species. Crop Sci 6:76–79
Lubbers EL, Gill KS, Cox TS, Gill BS (1991) Variation of molecular markers among geographically diverse accessions of Triticum tauschii. Genome 34:354–361
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Mann JA, Kimber CT, Miller FR (1983) The origin and early cultivation of sorghums in Africa. Bulletin, no. 1454. Texas A&M University, College Station, Texas, USA
Maunder AB, Sharp GI (1963) Localization of outcrosses within the panicle of fertile sorghum. Crop Sci 3:449
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Melchinger AE, Lee M, Lamkey KR, Hallauer AR, Woodman WL (1990) Genetic diversity for restriction fragment length polymorphisms and heterosis for two diallel sets of maize inbreds. Theor Appl Genet 80:488–496
Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448
Morden WC, Doebley J, Schertz KF (1989) Allozyme variation in old world races of Sorghum bicolor (Poaceae). Am J Bot 76:245–255
Morden WC, Doebley J, Schertz KF (1990) Allozyme variation among the spontaneous species of Sorghum section Sorghum (Poaceae). Theor Appl Genet 80:296–304
Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Reed KC, Mann AD (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nuclic Acids Res 13:7207–7221
Song KM, Osborn TC, William PH (1988) Brassica taxonomy based on nuclear restriction fragment polymorphisms (RFLPs). Theor Appl Genet 75:784–794
Song KM, Osborn TC, William PH (1990) Brassica taxonomy based on nuclear restriction fragment polymorphisms (RFLPs). 3 Genome relationships in Brassica and related genera and the origin of B. oleracea and B. rapa (syn. campestris). Theor Appl Genet 79:497–506
Swofford DL (1993) Phylogenetic Analysis Using Parsimony, version 3.1 Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois
Tao Y, Manners JM, Ludlow MM, Henzell RG (1993) DNA polymorphisms in grain sorghum [Sorghum bicolor (L.) Moench]. Theor Appl Genet 86:679–688
Wang ZY, Tanksley SD (1989) Restriction fragment length polymorphism in Oryza sativa L. Genome 32:1113–1118
Wet JMJ de (1978) Systematics and evolution of Sorghum sect. Sorghum (Gramineae). Am J Bot 65:477–484
Wet JMJ de, Huckabay JP (1967) Origin of Sorghum bicolor. II. Distribution and domestication. Evolution 21:787–802
Wet JMJ de, Prasada RKE (1986) Wild sorghums and their significance in crop improvement. Proc Sorghum Conf Shenyang China, pp 28–33
Wet JMJ de, Harlan JR, Price EG (1970) Origin of variability in the Spontanea complex of Sorghum bicolor. Am J Bot 57:704–707
Whitkus R, Doebley J, Lee M (1992) Comparative genome mapping of sorghum and maize. Genetics 132:1119–1130
Wright S (1931) Evolution in Mendelian populations. Genetics 16:97–159
Wright S (1932) The roles of mutation, inbreeding, crossbreeding and selection in evolution. Proc 6th Int Cong Genet 1:356–366
Wright S (1963) Genic Interaction. In: Burdette WJ (ed) Methodology in mammalian genetics. Holden-Day, San Francisco, pp 159–192
Wright S (1978) Evolution and the genetics of populations, vol 4. Variability within and among natural populations. University of Chicago Press, Chicago 580 pp
Xu GW, Magill CW, Hart GE (1993) Primers that amplify inserts in a multi-cloning site also hybridize to Sorghum bicolor (L.) Moench DNA. Genome 36:198–201
Xu GW, Magill CW, Schertz KF, Hart GE (1994) An RFLP linkage map of Sorghum bicolor (L.) Moench. Theor Appl Genet 89:139–145
Xu GW, Cui YX, Schertz KF, Hart GE (1995) Isolation of mitochondrial DNA sequences that distinquish male-sterility inducing cytoplasms in Sorghum bicolor (L.) Moench. Theor Appl Genet (in press)
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Communicated by J. Mac Key
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Cui, Y.X., Xu, G.W., Magill, C.W. et al. RFLP-based assay of Sorghum bicolor (L.) Moench genetic diversity. Theoret. Appl. Genetics 90, 787–796 (1995). https://doi.org/10.1007/BF00222013
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DOI: https://doi.org/10.1007/BF00222013