Summary
The origin of the vasopressinergic and oxytocinergic nerve fibres of the external region of the rat median eminence was investigated by means of hypothalamic lesions, adrenalectomy and immunocytochemistry. The results obtained in bilaterally adrenalectomized animals with complete, or incomplete, destruction of the suprachiasmatic nuclei showed that, at least, the great majority of the vasopressinergic and oxytocinergic nerve fibres of the external region of the rat median eminence do not originate from the suprachiasmatic nuclei. From the observations obtained in bilaterally adrenalectomized animals with total or subtotal destruction of both paraventricular hypothalamic nuclei, it appears that the paraventricular nuclei must be the origin of (nearly) all the vasopressinergic and oxytocinergic nerve fibres of the external region of the rat median eminence. The results strongly suggest that both types of fibres originate from all parts of the paraventricular nuclei.
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Aspeslagh, M.-R., Vandesande, F., Dierickx, K.: Electron microscopic immunocytochemical demonstration of separate neurophysin-vasopressinergic and neurophysin-oxytocinergic nerve fibres in the neural lobe of the rat hypophysis. Cell Tiss. Res. 171, 31–37 (1976)
Dierickx, K., Vandesande, F.: Immunocytochemical demonstration, in the external region of the amphibian median eminence, of separate vasotocinergic and mesotocinergic nerve fibres. Cell Tiss. Res. 177, 47–56 (1977)
Dierickx, K., Vandesande, F., De Mey, J.: Identification and origin of the vasopressin-neurophysin containing nerve fibres of the external region of the rat median eminence. Ann. Endocr. (Paris) 36, 383–384 (1975)
Dierickx, K., Vandesande, F., De Mey, J.: Identification, in the external region of the rat median eminence, of separate neurophysin-vasopressin and neurophysin-oxytocin containing nerve fibres. Cell Tiss. Res. 168, 141–151 (1976)
Krisch, B.: Immunohistochemical and electron microscopic study of the rat hypothalamic nuclei and cell clusters under various experimental conditions. Possible sites of hormone release. Cell Tiss. Res. 174, 109–127 (1976)
Mey, J. De, Dierickx, K., Vandesande, F.: Immunohistochemical demonstration of neurophysin I- and neurophysin II-containing nerve fibres in the external region of the bovine median eminence. Cell Tiss. Res. 157, 517–519 (1975a)
Mey, J. De, Dierickx, K., Vandesande, F.: Identification of neurophysin producing cells. III. Immunohistochemical demonstration of neurophysin I-producing neurons in the bovine infundibular nucleus. Cell Tiss. Res. 161, 219–224 (1975b)
Parry, H.B., Livett, B.G.: A new hypothalamic pathway to the median eminence containing neurophysin and its hypertrophy in sheep with natural scrapie. Nature (Lond.) 242, 63–65 (1973)
Skowsky, W.R., Fisher, D.A.: The use of thyroglobulin to induce anti-genicity to small molecules. J. Lab. clin. Med. 80, 134–144 (1972)
Sternberger, L.A., Hardy, P.H., Jr., Cuculis, J.J., Meyer, H.G.: The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)
Vandesande, F., De Mey, J., Dierickx, K.: Identification of neurophysin producing cells. I. The origin of the neurophysin-like substance-containing nerve fibres of the external region of the median eminence of the rat. Cell Tiss. Res. 151, 187–200 (1974)
Vandesande, F., Dierickx, K.: Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tiss. Res. 164, 153–162 (1975)
Vandesande, F., Dierickx, K.: Immuno-cytochemical demonstration of the inability of the homozygous Brattleboro rat to synthesize vasopressin and vasopressin-associated neurophysin. Cell Tiss. Res. 165, 307–316 (1976)
Vandesande, F., Dierickx, K., De Mey, J.: Identification of the vasopressin-neurophysin II and the oxytocin-neurophysin I producing neurons in the bovine hypothalamus. Cell Tiss. Res. 156, 189–200 (1975a)
Vandesande, F., Dierickx, K., De Mey, J.: Identification of the vasopressin-neurophysin producing neurons of the rat suprachiasmatic nuclei. Cell Tiss. Res. 156, 377–380 (1975b)
Vandesande, F., Dierickx, K., De Mey, J.: Identification of separate vasopressin-neurophysin II and oxytocin-neurophysin I containing nerve fibres in the external region of the bovine median eminence. Cell Tiss. Res. 158, 509–516 (1975c)
Zimmerman, E.A., Robinson, A.G.: Hypothalamic neurons secreting vasopressin and neurophysin. Kidney International 10, 12–24 (1976)
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This investigation was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk Onderzoek
The authors are much indebted to Prof. Dr. E. Kühn (Leuven) in whose laboratory the stereotactic operations were done
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Vandesande, F., Dierickx, K. & De Mey, J. The origin of the vasopressinergic and oxytocinergic fibres of the external region of the median eminence of the rat hypophysis. Cell Tissue Res. 180, 443–452 (1977). https://doi.org/10.1007/BF00220167
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DOI: https://doi.org/10.1007/BF00220167