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The prolactin and growth-hormone producing cells of the guinea-pig pituitary

Electron microscopic study using immunocytochemical means

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Summary

Different immunohistochemical techniques were used to identify the prolactin and growth hormone producing cells (STH cells) in the pituitary of normal guinea pigs at the ultrastructural level.

Prolactin cells revealed two main aspects: 1. Cells with granules from 2500 to 3500 Å in diameter some of which are irregularly shaped. These granulations are scattered throughout the whole cytoplasm. The rough endoplasmic reticulum is well developed and organized in parallel lamellae. 2. In cells of the second type the granules are less numerous and smaller in diameter (1800 to 2500 Å) and the rough endoplasmic reticulum is less well developed.

The cytoplasm of the STH cells contains many more round granules (generally grouped) which also range from 2500 to 3500 Å in diameter.

The prolactin molecules and the STH molecules are essentially confined to the granules but with the immunocytochemical technique before embedding a slightly diffuse reaction appeared in the entire cytoplasm. These results are discussed.

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References

  • Barnes, B.G.: Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinology 71, 618–628 (1962)

    Google Scholar 

  • Beauvillain, J-C., Mazzuca, M., Dubois, M.P.: The thyrotropic cells of the guinea pig pituitary. Electron microscopic study after characterization by immunocytochemical means. Cell Tiss. Res. 174, 233–244 (1976)

    Google Scholar 

  • Beauvillain, J-C., Tramu, G.: Cellules à activité corticotrope de l'hypophyse du Lérot (Eliomys quercinus): superpositions des résultats de microscopie optique (immunofluorescence et colorations) et de microscopie électronique. C.R. Acad. Sci. (Paris) 277, 1025–1028 (1973)

    Google Scholar 

  • Beauvillain, J-C., Tramu, G., Dubois, M.P.: Individualisation ultrastructurale des cellules gonadotropes et corticotropes de l'antéhypophyse du Lérôt et du cobaye par superposition de microscopie optique (immunofluorescence et coloration) et de microscopie électronique. J. de Microscopie 20, 20a (1974)

  • Beauvillain, J-C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in Lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)

    Google Scholar 

  • Bugnon, Cl., Lenys, D., Herlant, M., Dessy, C.: Caractérisation de diverses cellules de l'adénohypophyse du Renard par immunofluorescence sur coupes semi fines et superpositions des données de microscopie électronique. C.R. Acad. Sci. (Paris) 278, 1243–1248 (1974)

    Google Scholar 

  • Chang, N.G., Nikitowith-Winer, M.B.: Correlation between suckling induced changes in the ultrastructure of mammotrophs and prolactin release. Cell Tiss. Res. 166, 407–413 (1976)

    Google Scholar 

  • Dacheux, F., Dubois, M.P.: Ultrastructural localisation of prolactin, growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary. Cell Tiss. Res. 174, 245–260 (1976)

    Google Scholar 

  • Dekker, A.: Electron microscopic study of somatotropic and lactotropic pituitary cells in the Syrian hamster. Anat. Rec. 162, 123–136 (1968)

    Google Scholar 

  • Deslex, P., Rossi, G.L., Probst, D.: Ultrastructural study of the adenohypophysis of the Chinese hamster. Acta anat. (Basel) 96, 35–54 (1976)

    Google Scholar 

  • Doerr-Schott, J., Dubois, M.P.: Mise en évidence des hormones de l'hypophyse d'un amphibien par la cyto-immuno-enzymologie au microscope électronique. C.R. Acad. Sci. (Paris) 278, 1923–1926 (1974)

    Google Scholar 

  • Dubois, M.P.: Cytologie de l'hypophyse des bovins: séparation des cellules somatotropes et des cellules à prolactine par immunofluorescence. Identification des cellules LH dans la pars tuberalis et la pars intermedia. Bull. Ass. Anat. (Nancy) 145, 139–146 (1969)

    Google Scholar 

  • Dubois, M.P.: Etudes de l'apparition des sécrétions hormonales dans l'hypophyse foetale de bovin: mise en évidence par immunofluorescence des cellules somatotropes et des cellules à prolactine. C.R. Acad. Sci. (Paris) 272, 433–435 (1971)

    Google Scholar 

  • Dubois, M.P., Cohere, G.: Cytologie ultrastructurale du lobe antérieur et de la pars tuberalis de l'hypophyse des bovins. Bull. Ass. Anat. 145, 147–157 (1969)

    Google Scholar 

  • Farquhar, M.G., Rinehart, J.F.: Electron microscope studies of the anterior pituitary gland of castrated rats. Endocrinology 54, 526–541 (1954)

    Google Scholar 

  • Girod, C., Dubois, M.P.: Immunofluorescent identification of somatotropic and prolactin cells in the anterior lobe of the hypophysis of the monkey, Macacus irus. Cell Tiss. Res. 172, 145–148 (1976)

    Google Scholar 

  • Gomez-Dumm, C.L.A., Echavellanos, J.M.: Further studies on the ultrastructure of the pars distalis of the male mouse hypophysis. Acta anat. (Basel) 82, 254–261 (1972)

    Google Scholar 

  • Graham, R.C., Karnovsky, M.J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)

    Google Scholar 

  • Halmi, N.S., Parson, J.A., Erlandsen, S.L., Duello, T.: Prolactin and growth hormone cells in the human hypophysis: A study with immunoenzyme histochemistry and differential staining. Cell Tiss. Res. 158, 497–509 (1975)

    Google Scholar 

  • Hedinger, C.E., Farquhar, M.B.: Elektronenmikroskopische Untersuchungen von zwei Typen acidophiler Hypophysenvorderlappenzellen bei der Ratte. Schweiz. Z. Path. Bakt. 20, 766–768 (1957)

    Google Scholar 

  • Li, J.Y., Dubois, M.P., Dubois, P.M.: Immunocytochimie ultrastructurale de l'antéhypophyse foetale humaine. J. Microscopie Biol. Cell. 26, 18a (1976)

  • Martin-Comin, J., Robyn, C.: Comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries. J. Histochem. Cytochem. 24, 1012–1017 (1976)

    Google Scholar 

  • Mayor, H.D., Hampton, J.C., Rosario, B.: A simple method for removing the resin from epoxy embedded tissue J. biophys. biochetn. Cytol. 9, 909–910 (1961)

    Google Scholar 

  • Mazzuca, M.: Individualisation morphofonctionelle des terminaisons nerveuses périvasculaires de l'éminence médiane (chez les mammifères) en microscopie électronique. Réunion de la D.G.R.S.T. “Biologie de la Reproduction et du Développement”. Paris, Mai 1975

  • Mazzuca, M., Dubois, M.P.: Detection of luteinizing hormone releasing hormone in the guinea pig median eminence with an immunohistoenzymatic technique. J. Histochem. Cytochem. 22, 993–996 (1974)

    Google Scholar 

  • Mikami, S.: Light and electron microscopic investigation of six types of glandular cells of the bovine adenohypophysis. Z. Zellforsch., 105, 457–482 (1970)

    Google Scholar 

  • Moriarty, G.C., Halmi, N.S.: Electron microscopic study of the adrenocorticotropin-producing cell with the use of unlabeled antibody and the soluble peroxidase-antiperoxidase complex. J. Histochem. Cytochem. 20, 590–604 (1972)

    Google Scholar 

  • Moriarty, G.C., Tobin, R.B.: An immunocytochemical study of TSH storage in rat thyroidectomy cells with or without D or L thyroxine treatment. J. Histochem. Cytochem. 24, 1140–1149 (1976)

    Google Scholar 

  • Nakane, P.K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)

    Google Scholar 

  • Nakane, P.K.: Identification of anterior pituitary cells by immunoelectron microscopy. In “The anterior pituitary”, edit. by A. Tixier-Vidai and M.G. Farquhar. New York, Acad. Press, 1975

    Google Scholar 

  • Parsons, J.A., Erlandsen, S.L.: Ultrastructural immunocytochemical localization of prolactin in rat anterior pituitary by use of the unlabeled antibody enzyme method. J. Histochem. Cytochem. 22, 340–352 (1974)

    Google Scholar 

  • Petrali, J.P., Hinton, D.M., Moriarty, G.C., Sternberger, L.A.: The unlabeled antibody enzyme method of immunocytochemistry. Quantitative comparison of sensitivities with and without peroxidase antiperoxidase complex. J. Histochem. Cytochem. 22, 782–801 (1974)

    Google Scholar 

  • Racadot, J., Vila Porcile, E., Olivier, L., Peilion, F.: Electron microscopy of pituitary tumours. Prog. Neurol. Surg. 6, 95–141 (1975)

    Google Scholar 

  • Stefanini, M., De Martino, C., Zamboni, L.: Fixation of ejaculated spermatozoa for electron microscopy. Nature (Lond.) 216, 173 (1967)

    Google Scholar 

  • Tixier-Vidal, A., Tougard, C.: Subcellular localization of several anterior pituitary and hypothalamic hormones as revealed by the peroxidase labeled antibody method. Proceedings of the first International Symposium on Immunoenzymatic techniques, Paris 2nd–4th april 1975, edit, by G. Feldmann, P. Druet, J. Bignon and S. Avrameas.

  • Tramu, G., Beauvillain, J-C., Dubois, M.P.: Identification des cellules gonadotropes de l'hypophyse du Lérot (Eliomys quercinus) par une méthode de superposition d'observations de microscopie optique et de microscopie électronique. J. de Microscopie 21, 181–184 (1974)

    Google Scholar 

  • Young, B.A., Chaplin, R.E.: Some observations on the ultrastructure of the adenohypophysis of certain cervidae. J. Zool. (Lond.) 175, 493–508 (1975)

    Google Scholar 

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Authors and Affiliations

  1. U. 156 I.N.S.E.R.M. Laboratory of Histology of the Faculty of Medicine, Lille Cedex, France

    J. C. Beauvillain, M. Mazzuca & M. P. Dubois

  2. Laboratory of the Physiology of Reproduction I.N.R.A., C.N.R.Z., Nouzilly, France

    J. C. Beauvillain, M. Mazzuca & M. P. Dubois

Authors
  1. J. C. Beauvillain
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  2. M. Mazzuca
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  3. M. P. Dubois
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This work was supported by a grant from U.E.R. III Lille

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Beauvillain, J.C., Mazzuca, M. & Dubois, M.P. The prolactin and growth-hormone producing cells of the guinea-pig pituitary. Cell Tissue Res. 184, 343–358 (1977). https://doi.org/10.1007/BF00219895

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  • DOI: https://doi.org/10.1007/BF00219895

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