Skip to main content
Log in

Bioassays for incompatibility

  • Review
  • Published:
Sexual Plant Reproduction Aims and scope Submit manuscript

Summary

Self-incompatibility is a form of plant growth regulation acting on pollen and the pollen tube. It could therefore be amenable to study by bioassay techniques, which have been used successfully in the past to show the existence of other plant growth regulators and to assist in their purification. The genetics of self-incompatibility is well understood, and yet there have been difficulties in applying bioassays to the study of the supposed gene products. This review examines published accounts of attempts made to use the bioassay technique in the study of self-incompatibility. In general, bioassays for sporophytic incompatibility have been more successful than gametophytic tests, but none is entirely convincing. Despite this, the authors believe it is worthwhile for those involved with fertilization incompatibility research to persist in trying to improve the bioassay for use as an analytical tool.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderson MA, Cornish EC, Mau S-L, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roch PJ, Haley JD, Penschow JD, Niall HD, Tregear GW, Colghlan JL, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44

    Google Scholar 

  • Ar-Rushdi AH (1956) Inheritance in Nicotiana tabacum. XXVI. Sterility genes from Tomentosa species. J Genet 54:9–22

    Google Scholar 

  • Borriss H, Krolop H (1955) Über physiologische Wechselwirkungen zwischen Pollen verschiedener Pflanzenarten. Naturwissenschaften 42:301–302

    Google Scholar 

  • Bredemeyer GMM, Blaas J (1981) S-specific proteins in styles of self-incompatible Nicotiana alata. Theor Appl Genet 59:185–190

    Google Scholar 

  • Brewbaker J (1957) Pollen cytology and self-incompatibility systems in plants. J Hered 48:271–277

    Google Scholar 

  • Brewbaker JL, Majumder SK (1961) Cultural studies of the pollen population effect and the self-incompatibility inhibition. Am J Bot 48:457–464

    Google Scholar 

  • Brink RA (1942) The physiology of pollen. IV. Chemotropism: effects on growth of grouping grains; formation and function of callose plugs: summary and conclusion. Am J Bot 11:417–436

    Google Scholar 

  • Callan NW, Thompson MM (1986) Exogenous sugars overcome incompatibility in hazelnut (Corylus avellana L.). Theor Appl Genet 71:657–661

    Google Scholar 

  • Dickinson HG, Moriarty JF, Lawson JR (1982) Pollen-pistil interaction in Lilium longiflorum: the role of the pistil in controlling pollen tube growth following cross- and self-pollinations. Proc R Soc London Ser B 215:45–62

    Google Scholar 

  • Ehlers H (1961) Untersuchungen zur Ernährungsphysiologie der Pollenschläuche. Biol Zentralbl 70:432–451

    Google Scholar 

  • Ferrari TE, Wallace DH (1975) Germination of Brassica pollen and expression of incompatibility in vitro. Euphytica 24:757–765

    Google Scholar 

  • Franklin-Tong VE, Lawrence MJ, Franklin FCH (1988) An in vitro bioassay for the stigmatic product of the self-incompatibility gene in Papaver rhoeas L. New Phytol 110:109–118

    Google Scholar 

  • Franklin-Tong VE, Knuth E, Marmey P, Lawrence MJ, Franklin FCH (1989) Characterization of a stigmatic component from Papaver rhoeas L. which inhibits the specific activity of a self-incompatibility (S-) gene product. New Phytol 112:307–315

    Google Scholar 

  • Harris PJ, Anderson MA, Bacic A, Clarke AE (1984) Cell-cell recognition in plants with special reference to the pollen-stigma interaction. Oxford Surv Plant Mol Cell Biol 1:161–203

    Google Scholar 

  • Harris PJ, Weinhandle JA, Clarke AE (1989) Effect on in vitro growth of an isolated style glycoprotein associated from self-incompatibility in Nicotiana alata. Plant Physiol 89:360–367

    Google Scholar 

  • Hart RW, Turturro A (1987) Bioassay. In: Parker SP (ed) McGraw-Hill encyclopedia of science and technology, vol 2, 6th edn. McGraw-Hill, New York, p 507

    Google Scholar 

  • Herpen MMA van (1984) Extracts from styles developed at different temperatures and their effect on compatibility of Petunia hybrida in excised style culture. Acta Bot Neerl 33:195–203

    Google Scholar 

  • Heslop-Harrison J (1983) Self-incompatibility: phenomenology and physiology. Proc R Soc London Ser B 218:371–395

    Google Scholar 

  • Hodgkin T, Lyon GD (1982) Germination of Lilium and Petunia pollen on TLC plates and their inhibition by extracts from Brassica oleracea tissues. In: Mulcahy D, Ottaviano E (eds) Pollen biology and implications for plant breeding. Elsevier, New York, pp 343–350

    Google Scholar 

  • Hodgkin T, Lyon GD (1983) Detection of pollen germination inhibitors in Brassica oleracea tissue extracts. Ann Bot (London) 52:781–789

    Google Scholar 

  • Hodgkin T, Lyon GD (1986) The effect of Brassica oleracea stigma extracts on the germination of Brassica oleracea pollen in a thin layer chromatographic bioassay. J Exp Bot 37:406–411

    Google Scholar 

  • Jackson JF (1989) Borate control of protein secretion from Petunia pollen exhibits critical temperature discontinuities. Sex Plant Reprod 2:11–14

    Google Scholar 

  • Jahnen W, Lush WM, Clarke AE (1989) Inhibition of in vitro pollen tube growth by isolated S-glycoproteins of Nicotiana alata. Plant Cell 1:501–510

    Google Scholar 

  • Kamboj R, Jackson JF (1986) Self-incompatibility alleles control a low-molecular-weight, basic protein in pistils of Petunia hybrida. Theor Appl Genet 71:815–819

    Google Scholar 

  • Kroh M (1956) Genetische und entwicklungsphysiologische Untersuchungen über die Selbststerilität von Raphanus raphanistrum. Z Vererbungsl 87:365–384

    Google Scholar 

  • Lewis D (1976) Incompatibility in flowering plants. Receptors and recognition, vol 2, series A. Cuatrecasas and greaves. Chapman & Hall, London

    Google Scholar 

  • Lewis D (1979) Genetic versatibility of incompatibility in plants. N Z J Bot 17:637–644

    Google Scholar 

  • Li YQ, Linskens HF (1983) Neutral sugar composition of pollen tube walls of Lilium longiflorum. Acta Bot Neerl 32:457–445

    Google Scholar 

  • Linskens HF (1955) Physiologische Untersuchungen der Pollenschlauch-Hemmung selbststeriler Petunien. Z Bot 43:1–44

    Google Scholar 

  • Linskens HF (1975) The physiological basis of incompatibility in angiosperms. In: Duckett JG, Rasey PA (eds) The biology of male gamete. Biol J Linnean Soc 7 [Suppl 1]: 143–152

  • Linskens HF, Jackson JF (eds) (1985) Cell components: modern methods of plant analysis, vol 1. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Linskens HF, Jackson JF (eds) (1986) Immunology in plant sciences: modern methods of plant analysis, vol 4. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Nasrallah JB, Kao TH, Goldberg ML, Nasrallah ME (1985) A cDNA clone encoding and S-locus-specific glycoprotein from Brassica oleracea. Nature 318:263–267

    Google Scholar 

  • Nasrallah JB, Su-May YU, Nasrallah ME (1988) Self-incompatibility genes of Brassica oleracea. Expression, isolation and structure. Proc Natl Acad Sci USA 85:5551–5555

    Google Scholar 

  • Nettancourt D de (1977) Incompatibility in angiosperms. (Monographs in theoretical and applied genetis, vol 3.) Springer, Berlin Heidelberg New York

    Google Scholar 

  • Niimi Y (1982) Studies on the self-incompatibility of Petunia hybrida in excised style culture. An attempt at improving a technique in excised-style culture. Euphytica 31:787–793

    Google Scholar 

  • Niimi Y, Linskens HF (1983) Studies on the self-incompatibility of Petunia hybrida in excised style culture. Differences in self-incompatibility reaction among four clones. Theor Appl Genet 64:213–217

    Google Scholar 

  • Reeve DR, Crozier A (1980) Quantitative analysis of plant hormones. In: MacMillan J (ed) Hormonal regulation of development I. (Encyclopedia of plant physiology, NS, vol 9.) Springer, Berlin Heidelberg New York, pp 240–245

    Google Scholar 

  • Roberts IN, Gaude TC, Harrod G, Dickinson HG (1983) Pollenstigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self-incompatibility. Theor Appl Genet 65:231–238

    Google Scholar 

  • Savelli R, Caruso C (1940) Stimulation mutuelle dans la germination des grains de pollen de Nicotiana. C R Acad Sci (Paris) 210:184–186

    Google Scholar 

  • Schoch-Bodmer H (1932) Methoden zur Ermittlung der Wachstumsgeschwindigkeit der Pollenschläuche im Griffel. Verh Schweiz Naturforsch Ges, pp 368–370

  • Schwarzenbach FH (1964) Untersuchungen von wässrigen Extrakten aus Griffeln kurz- und langgriffliger Blüten von Primula obconica auf den Gehalt an sporenkeimungsaktiven Wirkstoffen. In: Linskens HF (ed) Pollen: physiology and fertilization. North Holland, Amsterdam, pp 225–229

    Google Scholar 

  • Sharma N, Shivanna KR (1982) Effects of pistil extracts on in vitro responses of compatible and incompatible pollen. Indian J Exp Biol 20:255–256

    Google Scholar 

  • Sharma N, Shivanna KR (1986) Self-incompatibility recognition and inhibition of Nicotiana alata. In: Mulcahy DL, Bergamini Mulcahy G, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, New York Berlin Heidelberg, pp 179–184

    Google Scholar 

  • Singh A, Paolillo DJ Jr (1989) Towards an in vitro bioassay for the self-incompatibility response in Brassica oleracea. Sex Plant Reprod 2:277–280

    Google Scholar 

  • Stanley RG, Linskens HF (1974) Pollen biology, biochemistry, management. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Straub J (1946) Zur Entwicklungsphysiologie der Selbststerilität von Petunia. Z Naturforsch 1:287–291

    Google Scholar 

  • Straub J (1947) Zur Entwicklungsphysiologie der Selbststerilität von Petunia II. Das Prinzip des Hemmungsmechanismus. Z Naturforsch 2:433–444

    Google Scholar 

  • Sukhada K Jayachandra (1980) Pollen allelopathy; a new phenomenon. New Phytol 84:739–746

    Google Scholar 

  • Tomková J (1959) Problems of autosterility in Nicotiana alata Link et Otto (Formation of the inhibitory factor in the pistil). Biol Plant (Praha) 1:328–329

    Google Scholar 

  • Williams EG, Ramm-Anderson B, Dumas C, Mau SL, Clarke AE (1982) The effect of isolated components of Prunus avium L. styles on in vitro growth of pollen tubes. Planta 156:517–519

    Google Scholar 

  • Wulff HD (1935) Vergleich zwichen Kultur- und Griffelpräparaten von Pollenschläuchen von Narthecium ossifragum. Beih Bot Cbl 54:83–98

    Google Scholar 

  • Yasuda S (1934) Physiological research on incompatibility on Petunia violacea. Bull Imp Coll Agric Morioko 20:1–95

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jackson, J.F., Linskens, H.F. Bioassays for incompatibility. Sexual Plant Reprod 3, 207–212 (1990). https://doi.org/10.1007/BF00202876

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00202876

Key words

Navigation