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Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng

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Summary

The effects of initial osmotic pressure (IOP) on the production of ginseng polysaccharide and ginseng saponin were studied in suspension cultures of Panax notoginseng cells. At higher IOP, the specific saponin production and intracellular carbohydrate storage were increased, while the plant cell volume, the consumption rates of major medium components and the specific cell growth rate were decreased. The specific production of polysaccharide was reduced with an increase of IOP from 4.45 to 5.18 atm, and levelled off at an even higher IOP.

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References

  • Chen, P.S., Toribara, T.Y., and Warner, H. (1956). Anal. Chem. 28, 1756–1758.

    Google Scholar 

  • Do, C.B., and Cormier, F. (1990). Plant Cell Rep. 9, 143–146.

    Google Scholar 

  • Gulik, W.M. van, Hoopen, H.J.G. ten, and Heijnen, J.J. (1992). Biotechnol. Bioeng. 40, 863–874.

    Google Scholar 

  • Hecht, U., and Mohr, H. (1990). Physiol. Plant. 78, 379–387.

    Google Scholar 

  • Li, X.G., Zheng, Y.H., Jia, J.H., and Sai, F. (1987). Zhongyao Tongbao 12(6), 40–41 (in Chinese).

    Google Scholar 

  • Kimball, S.L., Beversdorf, W.D., and Bingham, E.T. (1975). Crop Sci. 15, 750–752.

    Google Scholar 

  • Rudge, K., and Morris, P. (1986). The effect of osmotic stress on growth and alkaloid accumulation in Catharanthus roseus. In: Secondary Melabolism in Plant Cell Cultures, P. Morris, A.H. Scragg, A. Stafford, and M.W. Fowler, eds. pp. 75–81, London: Cambridge University Press.

    Google Scholar 

  • Salisbury, F.G., and Ross, C.R. (1969). Plant Physiology, California: Wadsworth Publishing Co.

    Google Scholar 

  • Thorpe, T.A., and Murashige, T. (1968). Science 160, 421–422.

    Google Scholar 

  • Yang, H., He, S., and Yang, M.J. (1992). Zhongguo Yaolixue Tongbao 8, 218–220 (in Chinese).

    Google Scholar 

  • Zhong, J.-J., Seki, T., Kinoshita, S., and Yoshida, T. (1991). Biotechnol. Bioeng. 38, 653–658.

    Google Scholar 

  • Zhong, J.-J., Xu, G.-R., and Yoshida, T. (1994). World J. Microbiol. Biotechnol. 10, 590–592.

    Google Scholar 

  • Zhong, J.-J., and Zhu, Q.-X. (1995). Appl. Biochem. Biotechnol. 55(sn2), in press.

  • Zhu, W.G., Guo, S.S., and Yi, Y.N. (1990). Hunan Yike Xuebao 16(2), 107–110 (in Chinese).

    Google Scholar 

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Zhang, YH., Zhong, JJ. & Yu, JT. Effect of osmotic pressure on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng . Biotechnol Lett 17, 1347–1350 (1995). https://doi.org/10.1007/BF00189224

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  • DOI: https://doi.org/10.1007/BF00189224

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