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Use of a two phase partitioning bioreactor for the biodegradation of phenol

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Biotechnology Techniques

Summary

A two phase organic-aqueous bioreactor system was used to degrade high concentrations of phenol by partitioning the inhibitory substrate into the aqueous phase at sub-inhibitory levels. Pseudomonas putida was used to degrade 4 g of phenol in a fermentor containing 0.5L of 2-undecanone, in which the phenol was dissolved, and 1L of medium in just over 48 hours. This system has the advantage of being self regulating in terms of phenol delivery to the aqueous phase in response to the rate of consumption by the cells, and it eliminates the problem of substrate inhibition commonly observed at high concentrations in a batch system.

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Authors and Affiliations

  1. Department of Chemical Engineering, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Lisa D. Collins & Andrew J. Daugulis

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  1. Lisa D. Collins
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  2. Andrew J. Daugulis
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Collins, L.D., Daugulis, A.J. Use of a two phase partitioning bioreactor for the biodegradation of phenol. Biotechnol Tech 10, 643–648 (1996). https://doi.org/10.1007/BF00168472

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  • DOI: https://doi.org/10.1007/BF00168472

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