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Bioconversion dynamics for bioremediation of chlorpyrifos pesticide using bacterial consortia in two immiscible liquid phase partitioning bioreactor

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Abstract

Two-phase partitioning bioreactor (TPPB) model is favoured based on the use of a biocompatible, immiscible organic solvent in which high concentrations of pesticides are dissolved at normal temperature and pressure (NTP). This compound partitions into the aqueous phase containing microbial cells due to the metabolic activities of the cells and the interfacial tension generated between the two phases to weaken their energy barrier. Thus, the present study was carried out for bioremediation of chlorpyrifos in TPPB using bacterial consortia as an aqueous phase and hexadecane as an organic phase. The study showed that 93.44% chlorpyrifos was degraded by bacterial consortia within the time period of 15 days, creating a higher wetting spontaneity with − 10.3833 kJ/mol/K Gibb’s free energy with a higher surface area. The two-phase systems could be useful to salt-out or salt-in the specific pollutant across the phases to maintain the higher surface area for an efficient degradation.

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Acknowledgements

We acknowledge the Central University of Gujarat, Gandhinagar, to fulfil the required facilities for the research work. The authors also wish to thank the Gujarat State Biotechnology Mission (GSBTM), Gandhinagar, for 16S rRNA sequencing facilities for identification of bacteria.

Funding

RKR was supported by fellowships from the University Grant Commission, New Delhi, India.

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Authors and Affiliations

  1. Uttaranchal College of Science and Technology, Dehradun, India

    Raman Kumar Ravi

  2. School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, Gujarat, India

    Raman Kumar Ravi & M. H. Fulekar

  3. School of Chemical Sciences, Central University of Gujarat, Gandhinagar, Gujarat, India

    Man Singh

  4. Center of Research for Development, Parul University, Vadodara, Gujarat, India

    M. H. Fulekar

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  1. Raman Kumar Ravi
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  2. Man Singh
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  3. M. H. Fulekar
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Correspondence to Raman Kumar Ravi.

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Significant statement

The two-phase systems were found effective for bioremediation of chlorpyrifos using cow dung bacterial consortia as novel source of biomass. The finding shows unique study, i.e., determination of interracial tension and Gibbs free energy that illustrated the bioremediation of chlorpyrifos in two-phase partitioning bioreactor. The system is able to degrade high concentration of chlorpyrifos, and thus can be used for environmental clean-up approaches.

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Ravi, R.K., Singh, M. & Fulekar, M.H. Bioconversion dynamics for bioremediation of chlorpyrifos pesticide using bacterial consortia in two immiscible liquid phase partitioning bioreactor. Biomass Conv. Bioref. 12, 1755–1769 (2022). https://doi.org/10.1007/s13399-021-01695-4

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  • DOI: https://doi.org/10.1007/s13399-021-01695-4

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