Abstract
A quantitative investigation of the effect of external mass transfer on the overall rate of biodegradation of Congo Red dye in a recirculating packed bed bioreactor (RPBB) was performed. The isolated Bacillus subtilis MN372379 species was immobilized on the packing material made of low-density polyethylene (LDPE) foam cubes in the RPBB. The overall dye removal rates were measured at varied recirculating flow conditions and an empirical correlation for the external mass transfer coefficient was determined. The observed rate of removal of Congo Red dye in the RPBB was found to be increasing with the recirculation rate. An empirical correlation to predict the non-dimensional Colburn factor was obtained as JD = 1.34 Re−0.3. The dimensionless correlation for mass transfer coefficient would enable the design and scale-up of an RPBB with minimal impact of external mass transfer resistance on the overall rate of dye bio-degradation.
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Banerjee, A., & Ghoshal, A. K. (2016). Biodegradation of phenol by calcium-alginate immobilized @@ Bacillus cereus in a packed bed reactor and determination of the mass transfer correlation. Journal of Environmental Chemical Engineering, 4(2), 1523–1529. https://doi.org/10.1016/j.jece.2016.02.012
Bilal, M., & Asgher, M. (2015). Sandal reactive dyes decolorization and cytotoxicity reduction using manganese peroxidase immobilized onto polyvinyl alcohol-alginate beads. Chemistry Central Journal, 9, 47. https://doi.org/10.1186/s13065-015-0125-0
Binupriya, A. R., Sathishkumar, M., Ku, C. S., & Yun, S.-I. (2010). Sequestration of Reactive Blue 4 by free and immobilized Bacillus subtilis cells and its extracellular polysaccharides. Colloids and Surfaces b: Biointerfaces, 76(1), 179–185. https://doi.org/10.1016/j.colsurfb.2009.10.031
Champagne, P.-P., & Ramsay, J. A. (2010). Dye decolorization and detoxification by laccase immobilized on porous glass beads. Bioresource Technology, 101(7), 2230–2235. https://doi.org/10.1016/j.biortech.2009.11.066
Chaturvedi, A., Rai, B. N., Singh, R. S., & Jaiswal, R. P. (2021a). A comprehensive review on the integration of advanced oxidation processes with biodegradation for the treatment of textile wastewater containing azo dyes. Reviews in Chemical Engineering, 38(6). https://doi.org/10.1515/revce-2020-0010
Chaturvedi, A., Rai, B. N., Singh, R. S., & Jaiswal, R. P. (2021b). A computational approach to incorporate metabolite inhibition in the growth kinetics of indigenous bacterial strain Bacillus subtilis MN372379 in the treatment of wastewater containing Congo Red dye. Applied Biochemistry and Biotechnology, 193(7), 2128–2144. https://doi.org/10.1007/s12010-021-03538-4
Chaturvedi, A., Rai, B. N., Singh, R. S., & Jaiswal, R. P. (2021c). Comparative toxicity assessment using plant and luminescent bacterial assays after anaerobic treatments of dyeing wastewater in a recirculating fixed bed bioreactor. Journal of Environmental Chemical Engineering, 9(4), 105466. https://doi.org/10.1016/j.jece.2021.105466
Cheng, C. W., Abdulla, R., Sridhar, R. R., & Ravindra, P. (2011). Determination of external mass transfer model for hydrolysis of jatropha oil using immobilized lipase in recirculated packed-bed reactor. Advances in Chemical Engineering and Science, 1, 289–298. https://doi.org/10.4236/aces.2011.14040
Córdoba, A., Vargas, P., & Dussan, J. (2008). Chromate reduction by Arthrobacter CR47 in biofilm packed bed reactors. Journal of Hazardous Materials, 151(1), 274–279. https://doi.org/10.1016/j.jhazmat.2007.10.072
Dizge, N., & Tansel, B. (2010). External mass transfer analysis for simultaneous removal of carbohydrate and protein by immobilized activated sludge culture in a packed bed batch bioreactor. Journal of Hazardous Materials, 184(1), 671–677. https://doi.org/10.1016/j.jhazmat.2010.08.090
Elizalde-González, M. P., Fuentes-Ramírez, L. E., & Guevara-Villa, M. R. G. (2009). Degradation of immobilized azo dyes by Klebsiella sp. UAP-b5 isolated from maize bioadsorbent. Journal of Hazardous Materials, 161(2), 769–774. https://doi.org/10.1016/j.jhazmat.2008.04.023
Fiol, N., Escudero, C., Poch, J., & Villaescusa, I. (2006). Preliminary studies on Cr(VI) removal from aqueous solution using grape stalk wastes encapsulated in calcium alginate beads in a packed bed up-flow column. Reactive and Functional Polymers, 66(8), 795–807. https://doi.org/10.1016/j.reactfunctpolym.2005.11.006
Fogler, H. S. (2006). External diffusion effect on heterogeneous reactions. In Elements of Chemical Reaction Engineering (4th ed. pp. 757–810). Prentice Hall International.
Geed, S. R., Kureel, M. K., Giri, B. S., Singh, R. S., & Rai, B. N. (2017). Performance evaluation of Malathion biodegradation in batch and continuous packed bed bioreactor (PBBR). Bioresource Technology, 227, 56–65. https://doi.org/10.1016/j.biortech.2016.12.020
Geed, S. R., Kureel, M. K., Prasad, S., Singh, R. S., & Rai, B. N. (2018). Novel study on biodegradation of malathion and investigation of mass transfer correlation using alginate beads immobilized Bacillus sp. S4 in bioreactor. Journal of Environmental Chemical Engineering, 6(2), 3444–3450. https://doi.org/10.1016/j.jece.2018.05.025
Gopinath, K. P., Murugesan, S., Abraham, J., & Muthukumar, K. (2009). Bacillus sp. mutant for improved biodegradation of Congo red: Random mutagenesis approach. Bioresource Technology, 100(24), 6295–6300. https://doi.org/10.1016/j.biortech.2009.07.043
Ip, A. W. M., Barford, J. P., & McKay, G. (2010). Biodegradation of Reactive Black 5 and bioregeneration in upflow fixed bed bioreactors packed with different adsorbents. Journal of Chemical Technology & Biotechnology, 85(5), 658–667. https://doi.org/10.1002/jctb.2349
Jadhav, S. B., Phugare, S. S., Patil, P. S., & Jadhav, J. P. (2011). Biochemical degradation pathway of textile dye Remazol red and subsequent toxicological evaluation by cytotoxicity, genotoxicity and oxidative stress studies. International Biodeterioration & Biodegradation, 65(6), 733–743. https://doi.org/10.1016/j.ibiod.2011.04.003
Junghanns, C., Neumann, J. F., & Schlosser, D. (2012). Application of the aquatic fungus Phoma sp. (DSM22425) in bioreactors for the treatment of textile dye model effluents. Journal of Chemical Technology & Biotechnology, 87(9), 1276–1283. https://doi.org/10.1002/jctb.3797
Kafshgari, M. H., Mansouri, M., Khorram, M., & Kashani, S. R. (2013). Kinetic modeling: A predictive tool for the adsorption of zinc ions onto calcium alginate beads. International Journal of Industrial Chemistry, 4(1), 5. https://doi.org/10.1186/2228-5547-4-5
Karagoz, B., Bayramoglu, G., Altintas, B., Bicak, N., & Yakup Arica, M. (2011). Amine functional monodisperse microbeads via precipitation polymerization of N-vinyl formamide: Immobilized laccase for benzidine based dyes degradation. Bioresource Technology, 102(13), 6783–6790. https://doi.org/10.1016/j.biortech.2011.03.050
Kathiravan, M. N., Karthiga Rani, R., Karthick, R., & Muthukumar, K. (2010). Mass transfer studies on the reduction of Cr(VI) using calcium alginate immobilized Bacillus sp. in packed bed reactor. Bioresource Technology, 101(3), 853–858. https://doi.org/10.1016/j.biortech.2009.08.088
Kathiravan, M. N., Praveen, S. A., Gim, G. H., Han, G. H., & Kim, S. W. (2014). Biodegradation of Methyl Orange by alginate-immobilized Aeromonas sp. in a packed bed reactor: external mass transfer modeling. Bioprocess and Biosystems Engineering, 37(11), 2149–2162. https://doi.org/10.1007/s00449-014-1192-7
Kim, J. C., Garzotto, F., Nalesso, F., Cruz, D., Kim, J. H., Kang, E., Kim, H. C., & Ronco, C. (2011). A wearable artificial kidney: Technical requirements and potential solutions. Expert Review of Medical Devices, 8(5), 567–579. https://doi.org/10.1586/erd.11.33
Kureel, M. K., Geed, S. R., Rai, B. N., & Singh, R. S. (2018). Novel investigation of the performance of continuous packed bed bioreactor (CPBBR) by isolated Bacillus sp. M4 and proteomic study. Bioresource Technology, 266, 335–342. https://doi.org/10.1016/j.biortech.2018.06.064
Manu, B., & Chaudhari, S. (2002). Anaerobic decolorisation of simulated textile wastewater containing azo dyes. Bioresource Technology, 82(3), 225–231. https://doi.org/10.1016/S0960-8524(01)00190-0
Mata, A., Pinheiro, H., & Lourenço, N. (2015). Effect of sequencing batch cycle strategy on the treatment of a simulated textile wastewater with aerobic granular sludge. Biochemical Engineering Journal, 104, 106. https://doi.org/10.1016/j.bej.2015.04.005
Mudliar, S., Banerjee, S., Vaidya, A., & Devotta, S. (2008). Steady state model for evaluation of external and internal mass transfer effects in an immobilized biofilm. Bioresource Technology, 99, 3468–3474. https://doi.org/10.1016/j.biortech.2007.08.001
Niebisch, C. H., Malinowski, A. K., Schadeck, R., Mitchell, D. A., Kava-Cordeiro, V., & Paba, J. (2010). Decolorization and biodegradation of reactive blue 220 textile dye by Lentinus crinitus extracellular extract. Journal of Hazardous Materials, 180(1), 316–322. https://doi.org/10.1016/j.jhazmat.2010.04.033
Osma, J. F., Toca-Herrera, J. L., & Rodríguez-Couto, S. (2010). Transformation pathway of Remazol Brilliant Blue R by immobilised laccase. Bioresource Technology, 101(22), 8509–8514. https://doi.org/10.1016/j.biortech.2010.06.074
Parasyris, A., Discacciati, M., & Das, D. B. (2020). Mathematical and numerical modelling of a circular cross-flow filtration module. Applied Mathematical Modelling, 80, 84–98. https://doi.org/10.1016/j.apm.2019.11.016
Parawira, W., Murto, M., Zvauya, R., & Mattiasson, B. (2006). Comparative performance of a UASB reactor and an anaerobic packed-bed reactor when treating potato waste leachate. Renewable Energy, 31(6), 893–903. https://doi.org/10.1016/j.renene.2005.05.013
Phugare, S. S., Kalyani, D. C., Patil, Av., & Jadhav, J. P. (2011). Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. Journal of Hazardous Materials, 186(1), 713–723. https://doi.org/10.1016/j.jhazmat.2010.11.049
Sahu, A. K., Conneely, T., Nüsslein, K. R., & Ergas, S. J. (2009). Biological perchlorate reduction in packed bed reactors using elemental sulfur. Environmental Science & Technology, 43(12), 4466–4471. https://doi.org/10.1021/es900563f
Sessa, D. J., & Palmquist, D. E. (2008). Effect of heat on the adsorption capacity of an activated carbon for decolorizing/deodorizing yellow zein. Bioresource Technology, 99(14), 6360–6364. https://doi.org/10.1016/j.biortech.2007.11.076
Sonwani, R. K., Giri, B. S., Jaiswal, R. P., Singh, R. S., & Rai, B. N. (2020). Performance evaluation of a continuous packed bed bioreactor: Bio-kinetics and external mass transfer study. Ecotoxicology and Environmental Safety, 201, 110860. https://doi.org/10.1016/j.ecoenv.2020.110860
Srinivasan, R., Kathiravan, M. N., & Gopinath, K. P. (2011). Degradation of Tectilon Yellow 2G by hybrid technique: Combination of sonolysis and biodegradation using mutant Pseudomonas putida. Bioresource Technology, 102(3), 2242–2247. https://doi.org/10.1016/j.biortech.2010.10.022
Swain, G., Sonwani, R. K., Giri, B. S., Singh, R. S., Jaiswal, R. P., & Rai, B. N. (2021a). A study of external mass transfer effect on biodegradation of phenol using low-density polyethylene immobilized Bacillus flexus GS1 IIT (BHU) in a packed bed bioreactor. Water and Environment Journal, 35(1), 285–294. https://doi.org/10.1111/wej.12626
Swain, G., Sonwani, R. K., Singh, R. S., Jaiswal, R. P., & Rai, B. N. (2021b). A comparative study of 4-chlorophenol biodegradation in a packed bed and moving bed bioreactor: Performance evaluation and toxicity analysis. Environmental Technology & Innovation, 24, 101821. https://doi.org/10.1016/j.eti.2021.101820
Tepe, O., & Dursun, A. Y. (2008). Combined effects of external mass transfer and biodegradation rates on removal of phenol by immobilized Ralstonia eutropha in a packed bed reactor. Journal of Hazardous Materials, 151(1), 9–16. https://doi.org/10.1016/j.jhazmat.2007.05.049
Wang, Y., Zhu, T., Wong, Y. J., Zhang, K., & Chang, M. (2023). Treatment performance of multistage active biological process (MSABP) reactor for saline sauerkraut wastewater: acclimatization, optimization and improvement. Bioprocess and Biosystems Engineering, 23. https://doi.org/10.1007/s00449-023-02877-2
Yang, Y., Hu, H., Wang, G., Li, Z., Wang, B., Jia, X., & Zhao, Y. (2011). Removal of malachite green from aqueous solution by immobilized Pseudomonas sp. DY1 with Aspergillus oryzae. International Biodeterioration & Biodegradation, 65(3), 429–434. https://doi.org/10.1016/j.ibiod.2011.01.007
Yeo, W. S., Yuniarto, A. (2019). The external mass transfer model for the hydrolysis of palm olein using immobilized lipase in a scaled-up recirculated packed-bed reactor. after anaerobic treatments of dyeing wastewater in a recirculating fixed bed bioreactor. Journal of Environmental Chemical Engineering, 7(4), 103185. https://doi.org/10.1016/j.jece.2019.103185
Zilouei, H., Guieysse, B., & Mattiasson, B. (2006). Biological degradation of chlorophenols in packed-bed bioreactors using mixed bacterial consortia. Process Biochemistry, 41(5), 1083–1089. https://doi.org/10.1016/j.procbio.2005.11.019
Acknowledgements
Author wants to acknowledge Prof. Ram Sharan Singh and Prof. Birendra Nath Rai for their helpful suggestions, and the department of chemical engineering and technology, IIT (BHU) for providing necessary laboratory facility to conduct the research activities.
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Conceptualization: Anuj Chaturvedi, Ravi Prakash Jaiswal;
Methodology: Ravi Prakash Jaiswal, Anuj Chaturvedi.
Formal analysis and investigation: Anuj Chaturvedi;
Writing—original draft preparation: Anuj Chaturvedi;
Writing—review and editing: Anuj Chaturvedi, Ravi Prakash Jaiswal;
Funding acquisition: Ravi Prakash Jaiswal;
Resources: Ravi Prakash Jaiswal; Ram Sharan Singh.
Supervision: Ravi Prakash Jaiswal;
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Chaturvedi, A., Jaiswal, R.P. Investigation of External Mass Transfer during Biodegradation of Congo Red Dye in a Recirculating Packed Bed Bioreactor. Water Air Soil Pollut 234, 360 (2023). https://doi.org/10.1007/s11270-023-06382-w
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DOI: https://doi.org/10.1007/s11270-023-06382-w