Summary
Glucose and glycerol at concentrations of 2 % negatively affected amylase synthesis in plate and submerged Streptomyces kanamyceticus cultures. This microorganism was insensitive to growth inhibition by glucose analogs and deregulated mutants were identified by a clearing zone around colonies grown on starch and glycerol or glucose, and selected. Three kinds of mutants were obtained: one insensitive to glucose (Mutant 41), another insensitive to glycerol repression (Mutant E) and the last (Mutant 29) an amylase-hyperproducing mutant, albeit regulated by glucose or glycerol like the wild type. The levels of glucokinase, an enzyme involved in catabolite regulation of Enterobacteria, were determined and results showed no differences between the parental strain and the mutants.
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References
Angell, S., Schwarz, E. and M.J. Bibb. 1992. Molecular Microbiol. 6, 2833–2844.
Barredo, J.L., Alvarez, E., Cantoral, J.M., Díez, B. and J.F. Martín. 1988. Antimic. Ag. and Chemother. 32, 1061–1067.
Chang, L.T., Behr, D.A. and R.P. Elander. 1980. Dev. Ind. Microbiol. 21, 233–243.
Demain, A.L. 1989. In: Regulation of Secondary Metabolism in Actinomycetes. Shapiro, S. (Ed.). CRC Press, p. 127–134.
Flores, M.E. and S. Sánchez. 1985. FEMS Microbiol. Lett. 26, 191–194.
Hodgson, D.A. 1982. J. Gen. Microbiol. 128, 2417–2430.
Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Bruton, C.J., Kieser, H.M., Lydiate, D.J., Smith, C.P., Ward, J.M. and Schrempf, H. 1985. Genetic Manipulation of Streptomyces: A Laboratory Manual. Norwich: John Innes Foundation.
Kubicek-Pranz, E.M., Vollenhofer, S., Fritscher, C., Plicka, A., Hampel, W.A. and C.P. Kubicek. 1991. Enz. and Microb. Technol. 13, 39–44.
Mazieres, N., Peire, M. and Penasse, L. 1981. J. Antibiotic. 34, 544–550.
Miller, L. 1959. Anal. Chem. 31, 426–428.
Montenecourt, B.S. and D.E. Eveleigh. 1977. Appl. and Environ. Microbiol. 34, 777–782.
Saier, M.H. Jr. 1989. Microbiol. Rev. 53, 109–120.
Seno, E.T. and K. Chater. 1983. J. Gen. Microbiol. 129, 1403–1413.
Solís, S., Flores, M.E. and C. Huitrón. 1990. Biotechnol. Letts. 12, 751–756.
Trumbly, R.J. 1992. Mol. Microbiol. 6, 15–21.
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Flores, M.E., Ponce, E., Rubio, M. et al. Glucose and glycerol repression of α-amylase in Streptomyces kanamyceticus and isolation of deregulated mutants. Biotechnol Lett 15, 595–600 (1993). https://doi.org/10.1007/BF00138547
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DOI: https://doi.org/10.1007/BF00138547