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The effects of spermidine synthesis inhibitors on in-vitro plant development

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Abstract

The spermidine synthesis inhibitors methylglyoxal bis-(guanylhydrazone) (MGBG) and dicyclohexylammonium sulfate (DCHA) were found to reduce growth and embryogenesis in wild carrot cultures. Cellular polyamine levels were also affected by the inhibitors, with spermidine levels being especially reduced by DCHA. Similarly, MGBG reduced organogenetic development of shoots on excised aspen hypocotyls. These data suggest that the polyamines, especially spermidine, play an important role in the growth and development of plants.

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References

  1. Altman A, Friedman R and Levin N (1982) Arginine and ornithine decarboxylases, the polyamine biosynthetic enzymes of Mung bean seedlings. Plant Physiol. 69: 876–879

    Google Scholar 

  2. Bitonti A, McCann P and Sjoerdsma A (1982) Restriction of bacterial growth by inhibition of polyamine biosynthesis by using monofluoromethylornithine, difluoromethylornithine and dicyclohexylammonium sulphate. Biochem J 208: 435–441

    Google Scholar 

  3. Cohen E, Arad S, Heimer Y and Mizrahi Y (1982) Participation of ornithine decarboxylase in early stages of tomato fruit development. Plant Physiol 70: 540–543

    Google Scholar 

  4. Feirer R, Mignon G and Litvay JD (1984) Arginine decarboxylase and polyamines required for embryogenesis in the wild carrot. Science 223: 1433–1435

    Google Scholar 

  5. Flores HE and Galston A (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69: 701–706

    Google Scholar 

  6. Fozard J, Part M, Prakash N, Grove J, Schechter P, Sjoerdsma A and Koch-Weiser J (1980) Ornithine decarboxylase: An essential role in early mammalian embryogenesis. Science 208: 505–508

    Google Scholar 

  7. Galston AW (1983) Polyamines as modulators of plant development. Bio-Science 6: 382–388

    Google Scholar 

  8. Heby O (1981) Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19: 1–20

    Google Scholar 

  9. Heimer Y, Mizrahi Y and Bachrach U (1979) Ornithine decarboxylase activity in rapidly proliferating plant cells. FEBS Lett 104: 146–148

    Google Scholar 

  10. Hibasami H, Tanaka M, Nagai J and Ikeda T (1980) Dicyclohexylamine, a potent inhibitor of spermidine synthetase in mammalian cells. FEBS Lett 116: 99–101

    Google Scholar 

  11. Hirawasa E and Suzuki Y (1983) Biosynthesis of spermidine in maize seedlings. Phytochemistry 22: 103–106

    Google Scholar 

  12. Kaur-Sawhney R, Shih S, Flores H and Galston A (1982) Relation of polyamine synthesis and titer to aging and senescence in oat leaves. Plant Physiol 69: 405–410

    Google Scholar 

  13. Lin P (1984) Polyamine metabolism and its relation to response of the aleurone layers of barley seeds to gibberellic acid. Plant Physiol 74: 975–983

    Google Scholar 

  14. Malmberg R and McIndoo J (1983) Abnormal floral development of a tobacco mutant with elevated polyamine levels. Nature 305: 623–625

    Google Scholar 

  15. Montague M, Armstrong T and Jaworski E (1979) Polyamine metabolism in embryonic cells of Daucus carota. I. Changes in intracellular content and rates of synthesis. Plant Physiol 62: 430–433

    Google Scholar 

  16. Montague M, Armstrong T and Jaworski E (1979) Polyamine metabolism in embryonic cells of Daucus carota. II. Changes in arginine decarboxylase activity. Plant Physiol 63: 341–345

    Google Scholar 

  17. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15: 473–497

    Google Scholar 

  18. Pegg A and McCann P (1982) Polyamine metabolism and function. Am J Physiol 243: C212-C221

    Google Scholar 

  19. Russell D and Lombardini J (1971) Polyamines: Enhanced SAM-decarboxylase in rapid growth systems, and the relationship between polyamine concentrations and RNA accumulation. Biochim Biophys Acta 240: 273–286

    Google Scholar 

  20. Serafini-Fracassini D (1984) Effects of polyamines and polyamine inhibitors on growth, differentiation and organogenesis in plant tissue culture. In: Calderera C and Bachrach U, eds. Advances in Polyamines in Biomedical Sciences 1984, pp 189–196. Bologna (Italy): CLUEB

    Google Scholar 

  21. Smith TA (1970) Putrescine, spermidine and spermine in higher plants. Phytochemistry 9: 1479–1486

    Google Scholar 

  22. Williams-Ashman H and Schenone A (1972) Methylglyoxal bis-(guanylhydrazone) as a potent inhibitor of mammalian and yeast S-adenosylmethionine decarboxylases. Biochem Biophys Res Commun 46: 288–295

    Google Scholar 

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Authors and Affiliations

  1. Forestry Division, The Institute of Paper Chemistry, P.O. Box 1038, 54912, Appleton, WI, USA

    R. P. Feirer, S. R. Wann & D. W. Einspahr

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  1. R. P. Feirer
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  2. S. R. Wann
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  3. D. W. Einspahr
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Feirer, R.P., Wann, S.R. & Einspahr, D.W. The effects of spermidine synthesis inhibitors on in-vitro plant development. Plant Growth Regul 3, 319–327 (1985). https://doi.org/10.1007/BF00117589

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  • DOI: https://doi.org/10.1007/BF00117589

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