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Background and overview of current sediment toxicity identification evaluation procedures

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Journal of Aquatic Ecosystem Health

Abstract

Laboratory bioassays can provide an integrated assessment of the potential toxicity of contaminated sediments to aquatic organisms; however, toxicity as a sole endpoint is not particularly useful in terms of identifying remedial options. To focus possible remediation (e.g., source control), it is essential to know which contaminants are responsible for toxicity. Unfortunately, contaminated sediments can contain literally thousands of potentially toxic compounds. Methods which rely solely on correlation to identify contaminants responsible for toxicity are limited in several aspects: (a) actual compounds causing toxicity might not be measured, (b) concentrations of potentially toxic compounds may covary, (c) it may be difficult to assess the bioavailability of contaminants measured in a sediment, and (d) interactions may not be accounted for among potential toxicants (e.g., additivity). Toxicity identification evaluation (TIE) procedures attempt to circumvent these problems by using toxicity-based fractionation procedures to implicate specific contaminants as causative toxicants. Phase I of TIE characterizes the general physio-chemical nature of sample toxicants. Phase II employs methods to measure toxicants via different analytical methods, and Phase III consists of techniques to confirm that the suspect toxicants identified in Phases I and II of the TIE actually are responsible for toxicity. These TIE procedures have been used to investigate the toxicity of a variety of samples, including sediments. Herein we present a brief conceptual overview of the TIE process, and discuss specific considerations associated with sediment TIE research. Points addressed include: (a) selection and preparation of appropriate test fractions, (b) use of benthic organisms for sediment TIE work, and (c) methods for the identification of common sediment contaminants.

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  1. U.S. Environmental Protection Agency, 6201 Congdon Blvd, 55804, Duluth, MN, USA

    G. T. Ankley

  2. Minnesota Pollution Control Agency, 320 West 2nd Street, 55802, Duluth, MN, USA

    M. K. Schubauer-Berigan

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Ankley, G.T., Schubauer-Berigan, M.K. Background and overview of current sediment toxicity identification evaluation procedures. J Aquat Ecosyst Stress Recov 4, 133–149 (1995). https://doi.org/10.1007/BF00116649

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