Skip to main content
SpringerLink
Log in

Cytogenetic mapping of marker genes on the chromosome elements C and E of Drosophila pseudoobscura and D. subobscura

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Enzyme loci, visible marker genes and λ-cloned DNA-sequences from a D. miranda library were mapped cytologically on the chromosome elements C and E of D. pseudoobscura and D. subobscura. New data are incorporated into the linkage maps of the two species. Homologous segments can now be localized in the polytene chromosomes with these markers. A comparison of the chromosome elements E of D. melanogaster and D. subobscura shows 12 conserved subsections which have been rearranged by paracentric inversions in the evolution of the two lineages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • AndersonW. W., AyalaF. J. & MichodR. E., 1977. Chromosomal and allozymic diagnosis of three species of Drosophila. J. Heredity 68: 71–74.

    Google Scholar 

  • AndersonW. W. & NormanR. A., 1980. Linkage map for currently available mutants of Drosophila pseudoobscura. Dros. Inf. Serv. 55: 191.

    Google Scholar 

  • AyalaF. J., PowellJ. R., TraceyM. L., MouraoC. A. & Pérez-SalasS., 1972. Enzyme variability in the Drosophila willistoni group. IV. Genic variation in natural populations of Droshophila willistoni. Genetics 70: 113–139.

    Google Scholar 

  • BenderW., SpiererP., & HognessD. S., 1983. Chromosomal walking and jumping to isolate DNA from the Ace and Rosy loci and the Bithorax complex in Drosophila melanogaster. J. mol. Biol. 168: 17–33.

    Google Scholar 

  • BeverleyS. M. & WilsonA. C., 1984. Molecular evolution in Drosophila and the higher Diptera. II. A time scale for fly evolution. J. mol. Evol. 21: 1–13.

    Google Scholar 

  • Böhm, I., 1986. Chromosomaler Umbau und Artdifferenzierung—Untersuchungen zur Chromosomenevolution bei Drosophila in 3 Arten der Untergattung Sophophora. Dissertation, Universität Tübingen.

  • BridgesC. B., 1935. Salivary chromosome maps. J. Hered. 26: 60–64.

    Google Scholar 

  • Carson, H. L. & Yoon, J. S., 1982. Genetics and evolution of Hawaiian Drosophila. In: M. Ashburner, H. L. Carson & J. N. Thompson, (eds): Genetics and biology of Drosophila, Vol. 3b, 297–344.

  • DavisB., 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121: 404–427.

    Google Scholar 

  • DobzhanskyTh. & EplingC., 1944. Contributions to the genetics, taxonomy, and ecology of Drosophila pseudoobscura and its relatives. Carnegie Inst. Washington Publ. 554: 1–83.

    Google Scholar 

  • Felger, I. & Pinsker, W., 1987. Histone gene transposition in the phylogeny of the Drosophila obscura group. Z. zool. Syst. Evolut.-Forsch., in press.

  • HarrisH. & HopkinsonD. A., 1976. Handbook of enzyme electrophoresis in human genetics. North-Holland Publishing Co., Amsterdam.

    Google Scholar 

  • KoskeT. & Maynard SmithJ., 1954. Genetics and cytology of Drosophila subobscura. X. The fifth linkage group. J. Genet. 52: 536–541.

    Google Scholar 

  • Kunze-MühlE. & MüllerE., 1958. Witere Untersuchungen über die chromosomale Struktur und die natürlichen Strukturtypen von Drosophila subobscura. Chromosoma 9: 559–570.

    Google Scholar 

  • LakovaaraS. & SauraA., 1972. Location of enzyme loci in chromosomes of Drosophila willistoni. Experientia 28: 355–356.

    Google Scholar 

  • LarrugaJ. M. & PinskerW., 1984. The importance of geographic isolation, structural rearrangement and speciation for the genetic divergence of chromosome O in Drosophila subobscura, Drosophila guanche and Drosophila madeirensis. Z. zool. Syst. Evolut.-forsch. 22: 103–113.

    Google Scholar 

  • LefevreG., 1976. A photographic representation and interpretation of the polytene chromosomes of Drosophila melanogaster salivary glands. In: M.Ashburner & E.Novitski, (eds), The genetics and biology of Drosophila, Vol. 1a, pp. 31–66. Academic Press, London.

    Google Scholar 

  • Lindsley, D. L. & Grell, E. H., 1968. Genetic variations of Drosophila melanogaster. Carnegie Institute of Washington Publ. N. 627.

  • Lindsley, D. L. & Zimm, G., 1985. The genome of Drosophila melanogaster. Part I: Genes A-K. Dros. Inf. Serv. 62.

  • LoukasM. & KrimbasC. B., 1980. Isozyme techniques in Drosophila subobscura. Dros. Inf. Serv. 55: 157–158.

    Google Scholar 

  • LoukasM., KrimbasC. B., Mavragani-TsipidouP. & KastritsisC. D., 1979. Genetics of Drosophila subobscura populations. VIII. Allozyme loci and their chromosome maps. J. Heredity 70: 17–26.

    Google Scholar 

  • MorataG. & KerridgeS., 1981. Sequential functions of the bithorax complex of Drosophila. Nature 290: 778–781.

    Google Scholar 

  • MorrisonW. J. & MacIntyreR. J., 1978. Cytogenetic localization of the Acid phosphatase-1 gene in Drosophila melanogaster. Genetics 88: 487–497.

    Google Scholar 

  • MullerH. J. 1940. Bearings of the Drosophila work on systematics. In: J.Huxley, (ed.), The new systematics, Clarendon Press, Oxford, pp. 185–268.

    Google Scholar 

  • O'BrienS. J. & MacIntyreR. J., 1978. Genetics and biochemistry of enzymes and specific proteins of Drosophila. In: M.Ashburner & T. R. F.Wright, (eds.), The genetics and biology of Drosophila, Vol. 2a, pp. 395–551. Academic Press, London.

    Google Scholar 

  • O'BrienS. J., SeuanezH. N. & WomackJ. E., 1985. On the evolution of genome organization in mammals. In: R. J.MacIntyre, (ed.), Molecular evolutionary genetics. Plenum Press, New York, pp. 519–589.

    Google Scholar 

  • OhnoS., 1973. Ancient linkage groups and frozen accidents. Nature 244: 259–262.

    Google Scholar 

  • PattersonJ T. & StoneW. S., 1952. Evolution in the genus Drosophila. MacMillan Co., New York.

    Google Scholar 

  • PinskerW. & SperlichD., 1979. Allozyme variation in natural populations of Drosophila subobscura along a north-south gradient. Genetica 50: 207–219.

    Google Scholar 

  • PinskerW. & SperlichD., 1984. Cytogenetic mapping of enzyme loci on chromosomes J and U of Drosophila subobscura. Genetics 108: 913–926.

    Google Scholar 

  • SchweizerD., 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307–324.

    Google Scholar 

  • SperlichD. & PinskerW., 1984. A Y-translocation method for localizing enzyme genes on Drosophila polytene chromosomes. Experientia 40: 203–206.

    Google Scholar 

  • SteinemannM., PinskerW. & SperlichD., 1984. Chromosome homologies within the Drosophila obscura group probed by in sity hybridization. Chromosoma 91: 46–53.

    Google Scholar 

  • SturtevantA. H. & NovitskiE., 1941. The homologies of the chromosome elements in the genus Drosophila. Genetics 26: 517–541.

    Google Scholar 

  • TanC. C., 1937. The cytological maps of the autosomes in Drosophila pseudoobscura. Z. Zellforsch mikroskop. Anat. 26: 439–461.

    Google Scholar 

  • Trear-ClemonsL. & DoaneW. W., 1984. Biochemical loci of the ‘Fruit Fly’ (Drosophila melanogaster). Dros. Inf. Serv. 60: 17–42.

    Google Scholar 

  • VoelkerR. A., OhnishiS. & LangleyC. H., 1979. Genetic and cytogenetic studies of four glycolytic enzymes in Drosophila melanogaster: aldolase, triosephosphate isomerase, 3-phosphoglycerate kinase, and phosphoglucomutase. Biochem. Genet. 17: 769–783.

    Google Scholar 

  • VoelkerR. A., OhnishiS., LangleyC. H., GauszJ. & GyurkovicsH., 1981. Genetic and cytogenetic studies of malic enzyme in Drosophila melanogaster. Biochem. Genet. 19: 525–534.

    Google Scholar 

  • YardleyD. G. & YardleyC. A., 1980. Linkage analysis of the Amylase, Acid phosphatase-3 and Peptidase-3 loci in Drosophila pseudoobscura. Dros. Inf. Serv. 55: 192.

    Google Scholar 

Download references

Author information

Author notes

  1. I. Böhm

    Present address: Institut für Humangenetik, Goßlerstr. 12d, D-3400, Göttingen, F.R.G.

  2. W. Pinsker

    Present address: Institut für Allgemeine Biologie, Währingerstr. 17, A-1090, Wien, Austria

Authors and Affiliations

  1. Institut Biologie II, Lehrbereich Populationsgenetik, Auf der Morgenstelle 28, D-7400, Tübingen, F.R.G.

    I. Böhm, W. Pinsker & D. Sperlich

Authors
  1. I. Böhm
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Pinsker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Sperlich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Böhm, I., Pinsker, W. & Sperlich, D. Cytogenetic mapping of marker genes on the chromosome elements C and E of Drosophila pseudoobscura and D. subobscura . Genetica 75, 89–101 (1987). https://doi.org/10.1007/BF00055252

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00055252

Keywords

Search

Navigation