Abstract
Inhibition of mango somatic embryo growth was inducedin vitro by treatments for 4 or more weeks with abscisic acid (0–100 μM ABA) with and without high osmolarity provided by mannitol (0–10%). High osmolarity and ABA significantly affected somatic embryo length, precocious germination and the production of good quality secondary somatic embryos. High osmolarity also affected root elongation. Abscisic acid was more effective in suppressing growth and development of ≥0.5 cm-length somatic embryos than smaller somatic embryos. Development beyond the heart stage was significantly inhibited by both ABA and mannitol treatments. The recovery of good quality somatic embryos was enhanced by high levels of ABA (100 μM) with and without mannitol (0–5%). Somatic embryos that had been pulsed with ABA were partially desiccated at different relative humidities. Weight loss was affected only by relative humidity; and ABA did not enhance desiccation tolerance.
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Abbreviations
- ABA:
-
Abscisic acid
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- MM1:
-
Mango maturation medium
- RH:
-
Relative humidity
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Pliego-Alfaro, F., Monsalud, M.J.R., Litz, R.E. et al. Effect of abscisic acid, osmolarity and partial desiccation on the development of recalcitrant mango somatic embryos. Plant Cell Tiss Organ Cult 44, 63–70 (1996). https://doi.org/10.1007/BF00045914
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DOI: https://doi.org/10.1007/BF00045914