Summary
Nineteen single-copy clones isolated from a PstI genomic library (cv. Maiden Plantain), and eight Vigna chloroplast DNA clones were used to probe total genomic DNA digests of 57 genotypes of Musa from India. The 19 genomic clones detected a total of 107 polymorphisms among the 57 genotypes. Principal coordinates and phenetic analyses of these data placed cultivars and species into distinct groups that were in general agreement with a previously published RAPD-based classification of these same plant materials. The 107 polymorphisms were sufficient to differentiate each clone from every other clone. Heterologous Vigna chloroplast DNA probes were used to characterize the cytoplasm of Musa cultivars and species. PCO analysis of these RFLPs were detected both within and between the generally recognized genome groups, indicating multiple hybridization pathways in the origin of hybrid clones. Data presented demonstrate that RFLPs are sufficiently abundant to classify Musa germplasm and that genetic relationships among Musa cultivars, based upon RFLP data, are in general agreement with relationships determined by analysis of morphology and RAPDs.
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Bhat, K.V., S.R., Bhat & K.P.S., Chandel, 1992a. Survey of isozyme polymorphism for clonal identification in Musa. I. Esterase, acid phosphatase and catalase. J. Hort. Sci. 67: 501–507.
Bhat, K.V., S.R., Bhat & K.P.S., Chandel, 1992b. Survey of isozyme polymorphism for clonal identification in Musa. II. Peroxidase, superoxide dismutase, shikimate dehydrogenase and malate dehydrogenase. J. Hort. Sci. 67: 737–744.
Bhat, K.V. & R.L. Jarret, 1994. Random amplified polymorphic DNA and genetic diversity in Indian Musa germplasm. Genetic Resources and Crop Evolution. (in press)
Bonner, J.W., R.M., Warner & J.L., Brewbaker, 1974. A chemosystematic study of Musa cultivars. HortScience 9: 325–328.
Chakravorty, A.K., 1951. Origin of the cultivated bananas of Southeast Asia. Indian J. Genet. 11: 34–46.
Faure, S., J.L., Noyer, F., Carreel, J.P., Horry, F., Bakry & C., Lanaud, 1994. Maternal inheritance of chloroplast genome and paternal inheritance of mitochondrial genome in bananas (Musa acuminata). Curr. Genet. 25: 265–269.
Gawel, N. & R.L., Jarret, 1991. Cytoplasmic genetic diversity in bananas and plantains. Euphytica 52: 19–23.
Gawel, N., R.L., Jarret & A.T., Whittemore, 1992. Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa. Theor. Appl. Genet. 84: 286–290.
Havey, M.J. & F.J., Muehlbauer, 1989. Variability for restriction fragment lengths and phylogenies in lentil. Theor. Appl. Genet. 77: 839–843.
Horry, J.P. & M., Jay, 1988. Distribution of anthocyanin in wild and cultivated banana varieties. Phytochemistry 27: 2667–2672.
IBPGR, 1991. Revised banana descriptors. IBPGR, Rome, Italy.
Jaccard, P., 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44: 223–270.
Jartet, R.L. (Ed.), 1989. Identification of genetic diversity in the geneus Musa. INIBAP, Montpellier.
Jarret, R.L. & R.E., Litz, 1986. Enzyme polymorphism in Musa acuminata Colla. J. Hered. 77: 183–186.
Jarret, R.L., N., Gawel & A.T., Whittemore, 1992. RFLP-based phylogeny of Musa species in Papua New Guinea. Theor. Appl. Genet. 84: 579–584.
Kaemmer, D., A., Afza, K., Weising, G., Kahl & F.J., Novak, 1993. Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana (Musa spp.). Bio Technology 10: 1030–1035.
Kochert, G., T., Halward, W.D., Branch & C.E., Simpson, 1991. RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor. Appl. Genet. 81: 565–570.
Lanaud, C., H., Tezenas du Montcel, M.P., Jolivot, J.C., Glaszmann & D. Gonzalezde, Leon, 1992. Variation of ribosomal gene spacer length among wild and cultivated bananas. Heredity 68: 147–156.
Lebot, V., K.M., Aradhya, R., Manshardt & B., Meilleur, 1993. Genetic relationships among cultivated bananas and plantains from Asia and the Pacific. Euphytica 67: 163–176.
Livini, C., P., Ajmone-Marsan, A.E., Melchinger, M.M., Messmer & M., Motto, 1992. Genetic diversity of maize inbred lines within and among heterotic groups revealed by RFLPs. Theor. Appl. Genet. 84: 17–25.
Nagamine, T., G.A., Todd, K.P., McCann, H.J., Newbury & B.V., Ford-Lloyd, 1989. Use of restriction fragment length polymorphism to fingerprint beets at the genotype and species level. Theor. Appl. Genet. 78: 847–851.
Neuhausen, S.L., 1992. Evaluation of restriction fragment length polymorphisms in Cucumis melo. Theor. Appl. Genet. 83: 379–384.
Palmer, J.D., G.P., Singh & D.T.N., Pillay, 1987. Structure and sequence evolution of three legume chloroplast DNAs. Molec. Gen. Genet. 190: 13–19.
Saghai-Maroof, M.A., K.M., Soliman, R.A., Jorgenson & R.W., Allard, 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromsomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014–8018.
Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular cloning — a laboratory manual. 2nd ed., Cold Spring Harbor, NY, USA.
Shanmugavelu, K.G., K., Aravindakshan & S., Sathiamoorthy, 1992. Banana taxonomy, breeding and production technology. Metropolitan Book Co., New Delhi, India.
Simmonds, N.W., 1962. The evolution of the bananas. Longmans, Green and Co., Ltd., London.
Simmonds, N.W. & K., Shepherd, 1955. The taxonomy and origins of the cultivated bananas. J. Linn. Soc. (Bot.) London 55: 302–312.
Stover, R.H. & N.W., Simmonds, 1989. Bananas. 3rd ed. Longmans, London.
Swennen, R., 1990. Limits of morphotaxonomy: Names and synonyms of plantain in Africa and elsewhere. In: R.L., Jarret (Ed.). Identification of genetic diversity in the genus Musa, pp. 172–210. INIBAP, Montpellier, France.
Tanksley, S.D., J., Miller, A., Paterson & R., Bernatsky, 1987. Molecular mapping of plant chromosomes. In: J.P., Gustafson & R.A., Appels (Eds). Chromosome structure and function, pp. 157–173. Plenum Press, New York.
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Bhat, K.V., Jarret, R.L. & Liu, ZW. RFLP characterization of Indian Musa germplasm for clonal identification and classification. Euphytica 80, 95–103 (1994). https://doi.org/10.1007/BF00039303
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DOI: https://doi.org/10.1007/BF00039303