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Production of doubled haploid rice plants (Oryza sativa L.) by anther culture

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Abstract

The results of anther culture of F2 pollen issued from 23 single crosses are presented. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4°C), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg l−1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3 mg l−1 kinetin + 0.5 mg l−1 naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling. Thirtynine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses.

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  1. Centro Nacional de Pesquisa de Arroz e Feijão/Empresa Brasileira de Pesquisa Agropecuária, Caixa Postal 179, 74000, Goiânia, GO, Brasil

    Isabelle Reiffers & Adelson B. Freire

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  1. Isabelle Reiffers
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  2. Adelson B. Freire
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Reiffers, I., Freire, A.B. Production of doubled haploid rice plants (Oryza sativa L.) by anther culture. Plant Cell Tiss Organ Cult 21, 165–170 (1990). https://doi.org/10.1007/BF00033437

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